中国抗生素杂志
中國抗生素雜誌
중국항생소잡지
CHINESE JOURNAL OF ANTIBIOTICS
2010年
2期
128-133
,共6页
华德兴%彭青%黄源春%姚芬%钱元恕
華德興%彭青%黃源春%姚芬%錢元恕
화덕흥%팽청%황원춘%요분%전원서
耐甲氧西林金葡菌%抗菌药物%表型%基因型
耐甲氧西林金葡菌%抗菌藥物%錶型%基因型
내갑양서림금포균%항균약물%표형%기인형
Methicillin resistant S.aureus%Antibacterial agents%Phenotype%Genotype
目的 了解来自汕头和北京地区的67株耐甲氧西林金葡菌(MRSA)的耐药情况及耐药机制,并分析耐药表型与基因型的一致情况.方法 以琼脂稀释法测定24种抗菌药物对MRSA的体外抗菌活性,PCR扩增MRSA的耐药基因,nitrocefin测定细菌产β-内酰胺酶的情况.结果 67株MRSA除了对氯霉素和多西环素的耐药率较低,分别为3%和7.5%,对万古霉素、米诺环素和利凡诺100%敏感外,对利福平的耐药率为52.2%,对四环素的耐药率为77.6%,而对于其它包括青霉素、第1～4代头孢菌素、单环β-内酰胺类、碳青霉烯类、氨基糖苷类和磺胺类的耐药率均在80%～90%以上,氨苄西林加酶抑制剂以后则耐药程度有所降低.qacA/B、mecA、tetM、NorA、gyrA、aac(6')/aph(2")、aph(3')Ⅲ和β-内酰胺酶检出率分别为11.9%、95.5%、70.1%、4.5%、10.4%、82.1%、73.1%和92.5%,未检出qacC、ant(6)-I、ant(2")-I、VanA和VanB耐药基因.67株MRSA均携带有多种耐药基因:同时携带2种耐药基因的有7株,3种的有10K,4种的有11株,5种的29株,6种的10株.结论 67株MRSA耐药情况严重,并同时携带多种耐药基因.
目的 瞭解來自汕頭和北京地區的67株耐甲氧西林金葡菌(MRSA)的耐藥情況及耐藥機製,併分析耐藥錶型與基因型的一緻情況.方法 以瓊脂稀釋法測定24種抗菌藥物對MRSA的體外抗菌活性,PCR擴增MRSA的耐藥基因,nitrocefin測定細菌產β-內酰胺酶的情況.結果 67株MRSA除瞭對氯黴素和多西環素的耐藥率較低,分彆為3%和7.5%,對萬古黴素、米諾環素和利凡諾100%敏感外,對利福平的耐藥率為52.2%,對四環素的耐藥率為77.6%,而對于其它包括青黴素、第1～4代頭孢菌素、單環β-內酰胺類、碳青黴烯類、氨基糖苷類和磺胺類的耐藥率均在80%～90%以上,氨芐西林加酶抑製劑以後則耐藥程度有所降低.qacA/B、mecA、tetM、NorA、gyrA、aac(6')/aph(2")、aph(3')Ⅲ和β-內酰胺酶檢齣率分彆為11.9%、95.5%、70.1%、4.5%、10.4%、82.1%、73.1%和92.5%,未檢齣qacC、ant(6)-I、ant(2")-I、VanA和VanB耐藥基因.67株MRSA均攜帶有多種耐藥基因:同時攜帶2種耐藥基因的有7株,3種的有10K,4種的有11株,5種的29株,6種的10株.結論 67株MRSA耐藥情況嚴重,併同時攜帶多種耐藥基因.
목적 료해래자산두화북경지구적67주내갑양서림금포균(MRSA)적내약정황급내약궤제,병분석내약표형여기인형적일치정황.방법 이경지희석법측정24충항균약물대MRSA적체외항균활성,PCR확증MRSA적내약기인,nitrocefin측정세균산β-내선알매적정황.결과 67주MRSA제료대록매소화다서배소적내약솔교저,분별위3%화7.5%,대만고매소、미낙배소화리범낙100%민감외,대리복평적내약솔위52.2%,대사배소적내약솔위77.6%,이대우기타포괄청매소、제1～4대두포균소、단배β-내선알류、탄청매희류、안기당감류화광알류적내약솔균재80%～90%이상,안변서림가매억제제이후칙내약정도유소강저.qacA/B、mecA、tetM、NorA、gyrA、aac(6')/aph(2")、aph(3')Ⅲ화β-내선알매검출솔분별위11.9%、95.5%、70.1%、4.5%、10.4%、82.1%、73.1%화92.5%,미검출qacC、ant(6)-I、ant(2")-I、VanA화VanB내약기인.67주MRSA균휴대유다충내약기인:동시휴대2충내약기인적유7주,3충적유10K,4충적유11주,5충적29주,6충적10주.결론 67주MRSA내약정황엄중,병동시휴대다충내약기인.
Objective To investigate resistance to antibiotics and its mechanism of sixty-seven methicillin resistant Staphylococcus aureus(MRSA)strains from Shantou and Beijing,and to analyze the coherence between resistant phenotype and genotype.Methods The antimicrobial activities of twenty-four kinds of antibiotics against MRSA in vitro were performed by agar dilution method.Gene encoding resistance were detected by PCR and the production of β-latamases were determined with nitrocefin.Results All isolates remained 100%sensitive to vancocin,minocycline and rivanol.Resistance rate to chloromycetin and doxycycline(3%and 7.5%respectively)was also low.Except for that,resistance rate to rifampicin and tetracycline were 52.2%and 77.6%respectively.As for resistance rate to other antibiotics such as penicillin,the first to fourth generation cephalosporin,monobactams,carbopenems,aminoglycosides,and sulfamido were all above 80%to 90%.Resistance to ampicillin decreased when β-1actamse inhibitor was added.The prevalence of qacA/B,mecA,tetM,NorA,gyrA,aac(6')/aph(2"),aph(3')Ⅲ and β-lactamasewere 11.9%,95.5%,70.1%,4.5%,10.4%,82.1%,73.1%and 92.5%respectively.The gene of qacC,ant(6)-Ⅰ,ant(2")-Ⅰ,VanA and VanB were not detected.All isolates carried more than one resistance genes:seven strains for two resistance genes,ten strains for three resistance genes,eleven strains for four resistance genes,twentynine strains for five resistance genes,and ten strains for six resistance genes were deteced.Conclusions Drug resistance were serious in sixty-seven MRSA strains,and at the same time they all carried multiple resistance genes.
