CAJ | 학술논문

为了确定猪体细胞克隆胚胎的受体母猪最佳发情阶段,本研究比较了供受体同期化方案对猪体细胞克隆胚胎输卵管内移植后30 d受胎率和妊娠足月率的影响.将体外培养2d的猪体细胞克隆胚胎,移植到发情第1～2天的受体母猪.根据受体母猪卵泡发育与排卵状况将受体猪群分为2个类别:卵泡发育处于即将排卵或正在排卵阶段(组一);卵泡发育处于排卵前阶段或完全排卵阶段(组二).结果发现:(1)组-6头受体母猪胚胎移植后30d受胎率为100%;而组二10头受体母猪受胎率为40%,二者差异显著(P<0.05).(2)组-6头受体母猪全部产仔,妊娠足月率为100%;组二10头受胎母猪2头产仔,妊娠足月率为20%,二者差异极显著(P<0.01).以上结果表明:体外培养2 d的猪体细胞克隆胚胎发育阶段与即将排卵或正在排卵的受体母猪的子宫环境同期性最好,得到了理想的胚胎移植效率.
위료학정저체세포극륭배태적수체모저최가발정계단,본연구비교료공수체동기화방안대저체세포극륭배태수란관내이식후30 d수태솔화임신족월솔적영향.장체외배양2d적저체세포극륭배태,이식도발정제1～2천적수체모저.근거수체모저란포발육여배란상황장수체저군분위2개유별:란포발육처우즉장배란혹정재배란계단(조일);란포발육처우배란전계단혹완전배란계단(조이).결과발현:(1)조-6두수체모저배태이식후30d수태솔위100%;이조이10두수체모저수태솔위40%,이자차이현저(P<0.05).(2)조-6두수체모저전부산자,임신족월솔위100%;조이10두수태모저2두산자,임신족월솔위20%,이자차이겁현저(P<0.01).이상결과표명:체외배양2 d적저체세포극륭배태발육계단여즉장배란혹정재배란적수체모저적자궁배경동기성최호,득도료이상적배태이식효솔.
In this study,the effect of different ovulation status on pregnancy rates in surrogate recipient gilts receiving somatic nuclear transfer embryos was evaluated,in order to determine the optimal stage of the estrus cycle to achieve the highest pregnancy rates.Porcine somatic cell cloned embryos on 2 d in vitro were transplanted into the 1-2 d estrous recipient gilts.Recipient gilts were divided into two categories according to follicular development and ovulation status:just prior to ovulation or ovulating (Group 1);per-ovulation or after ovulation(Group 2).The results were as follows: (1) pregnancy rate on 30 d of six recipient gilts from Group 1 was 100 %;and the pregnancy rate on 30 d of ten recipient gilts from Group 2 was 40 %,the data significantly different between Group 1 and Group 2 (P<0.05) ;(2) For the full-term pregnancy rate of recipient gilts,extremely significant difference was observed between Group 1 and Group 2 (100 %vs.20%,P<0.01).These results suggested that development stage of porcine somatic cloning em bryos on 2 d in vitro with recipient gilt's womb environment of ovulation status of just prior to ovulation or ovulating was the best in the same period.It may achieve the best efficiency of embryo transfer.