CAJ | 학술논문

目的探讨刚地弓形虫能否通过血睾屏障侵入小鼠的睾丸及附睾组织.方法选用9～10周龄雄性BALB/c小鼠20只,随机分为感染组(16只)和正常对照组(4只),其中感染组小鼠每只腹腔注射含2.5×103 个弓形虫速殖子的PBS 0.2 ml,建立弓形虫致小鼠感染的动物模型,对照组给予等量的无菌PBS.分别于攻击感染后第1、3、5、7天各处死4只感染组小鼠、1只正常对照组小鼠,取睾丸及附睾,应用RNA原位杂交法检测睾丸及附睾组织中的弓形虫.结果对照组及感染后第1天的感染组小鼠睾丸及附睾中未检出速殖子;实验组在感染后第3天小鼠睾丸及附睾内偶尔可见弓形虫速殖子,以后随着感染时间的延长睾丸及附睾中的弓形虫速殖子数量逐渐增多,且不同感染时间点同一组织内的弓形虫速殖子的数目差别具有显著的统计学意义.结论刚地弓形虫可通过血睾屏障侵入小鼠的睾丸及附睾组织中.
목적 탐토강지궁형충능부통과혈고병장침입소서적고환급부고조직.방법 선용9～10주령웅성BALB/c소서20지,수궤분위감염조(16지)화정상대조조(4지),기중감염조소서매지복강주사함2.5×103 개궁형충속식자적PBS 0.2 ml,건립궁형충치소서감염적동물모형,대조조급여등량적무균PBS.분별우공격감염후제1、3、5、7천각처사4지감염조소서、1지정상대조조소서,취고환급부고,응용RNA원위잡교법검측고환급부고조직중적궁형충.결과 대조조급감염후제1천적감염조소서고환급부고중미검출속식자;실험조재감염후제3천소서고환급부고내우이가견궁형충속식자,이후수착감염시간적연장고환급부고중적궁형충속식자수량축점증다,차불동감염시간점동일조직내적궁형충속식자적수목차별구유현저적통계학의의.결론 강지궁형충가통과혈고병장침입소서적고환급부고조직중.
Objective To investigate the possibility of invasion of Toxoplasma gondii into testis and epididymis through blood-testis barrier. Methods Twenty BALB/c male mice(9-10 weeks old)were ran domly divided into two groups:experimental group and negative control group.The control group Was injected intraperitoneally with PBS while the experimental group Was inoculated intraperitoneally with 2.5×103 tachyzoites of T. gondii ( RH strain).Four mice in experimental group and 1 in control group were sacrificed at the 1st.3rd,5th and 7th day after infection.The testes and epididymides were collected and the paraffin sections were prepared to perform in situ hybridization. Results Tachyzoites were observed on sections of testes and epididymides 3,5 and 7 days after infection.The number of tachyzoites in the testes and epididymides increased with the infection time.Significant difference in the number of tachyzoites was found among the testes and epididymides at different infection time. Conclusion It was indicated that T. gondii Could invade into the testis and epididymis of infectious mice.