CAJ | 학술논문

目的为了能最大程度获得高质量和足够数量的精子,根据《WHO人类精液检验与处理实验室手册》第五版要求对体外精液优选技术进行了改进,以期为患者提供一种更安全、可靠、高效的精液优选方法.方法收集2011年l至9月广东省中医院生殖医学科共计68对不育夫妇的98份精液标本,采用改良前后的精液优选方法(密度梯度法和上游法)分别进行精液优选处理.优选前后使用改良Neubauer血细胞计数器进行精子浓度分析,西班牙SCA精子分析仪测定精子活动力,改良巴氏染色进行精子形态学分析.结果改良后两种方法回收的精液总活动精子数、前向运动(a+b)精于总数、回收率有所提高(P＜ 0.05)；其精子浓度、正常形态精子百分率未有明显变化(P＞0.05).优选前后比较正常形态精子有显著提高(P＜ 0.01).结论改良后精液优选技术能提高患者的前向运动精子总数及回收率,有效提高不育夫妇受孕的机率.
목적 위료능최대정도획득고질량화족구수량적정자,근거《WHO인류정액검험여처리실험실수책》제오판요구대체외정액우선기술진행료개진,이기위환자제공일충경안전、가고、고효적정액우선방법.방법 수집2011년l지9월광동성중의원생식의학과공계68대불육부부적98빈정액표본,채용개량전후적정액우선방법(밀도제도법화상유법)분별진행정액우선처리.우선전후사용개량Neubauer혈세포계수기진행정자농도분석,서반아SCA정자분석의측정정자활동력,개량파씨염색진행정자형태학분석.결과 개량후량충방법회수적정액총활동정자수、전향운동(a+b)정우총수、회수솔유소제고(P＜ 0.05)；기정자농도、정상형태정자백분솔미유명현변화(P＞0.05).우선전후비교정상형태정자유현저제고(P＜ 0.01).결론 개량후정액우선기술능제고환자적전향운동정자총수급회수솔,유효제고불육부부수잉적궤솔.
Objective To improve in vitro sperm selection technology basing on WHO lab mamual for Examination and Process of Human Semen ( fifth edition ) to offer patients with a safer,more reliable and effective sperm selection procedure.Methods 98 specimens from 68 sterility couples were collected.The specimen were detected by the old selection technology and the modified technology ( Direct swim-up and Discontinuous density-gradients ).Neubauer Blood Cell counter was used to detect the sperm concentration.Spain SCA-CASA was used to assess sperm motility and Papanicolaou Staining was used to detect sperm morphology.Results The forward sperm quantity and recovery improved after modification ( P＜ 0.05 ).The sperm concentration and normal forms of sperm did not change after modification ( P ＞ 0.05 ).The count of sperm with normal forms increased after selection ( P ＜ 0.01 ).Conclusions Modified sperm selection technology can improve the quantity of forward sperm and the recovery rate.It can increase the chance of conception in sterility couples.