CAJ | 학술논문

目的利用组织工程骺板软骨修复兔骺板缺损,防止肢体畸形发生.方法取2周龄兔骺板软骨细胞,体外复合牛关节软骨细胞外基质后植入4周龄兔右侧股骨远端骺板缺损处,左侧仅造成缺损,无填充物,作为自身空白对照.于每周行X线检查,4、8、12、16周时行组织学和免疫组化检查.结果 X线显示移植侧股骨畸形明显比对侧轻,骺板缺损处未见明显骨桥生成,被结构紊乱的软骨组织填充,具有一定的生长能力.空白对照侧骨桥形成,骺板早闭.组织学显示移植侧骺板较窄,排列紊乱.免疫组化结果显示移植处染色阳性.结论组织工程方法培养出骺板软骨不仅可以阻止骨桥产生,还具有一定的生长能力,有望成为治疗骺板早闭的一种合适的材料.
목적 이용조직공정후판연골수복토후판결손,방지지체기형발생.방법 취2주령토후판연골세포,체외복합우관절연골세포외기질후식입4주령토우측고골원단후판결손처,좌측부조성결손,무전충물,작위자신공백대조.우매주행X선검사,4、8、12、16주시행조직학화면역조화검사.결과 X선현시이식측고골기형명현비대측경,후판결손처미견명현골교생성,피결구문란적연골조직전충,구유일정적생장능력.공백대조측골교형성,후판조폐.조직학현시이식측후판교착,배렬문란.면역조화결과현시이식처염색양성.결론 조직공정방법배양출후판연골불부가이조지골교산생,환구유일정적생장능력,유망성위치료후판조폐적일충합괄적재료.
Objective The aim of this study is to establish the feasibility of acellular cartilage matrix as a scaffold for epiphyseal chondrocytes to form tissue engineering growth plate for the treatment of partial growth plate injury. Methods We collected and cultured the epiphyseal plate chondrocytes from 2 weeks old rabbit in vitro, seeded in acellular cartilage matrix to form a complex. It was then transferred to the rabbit right distal femur which was subjected to growth plate injury. The left femur was subjected to the same injury without further treatment (control). The femur recovery was monitored with weekly X-ray. The morphology was studied with hematoxylin- eosin stain and immunohistochemistry. Results The right femur recovery was significantly better than that of the left radiologically. Histology and immunohistochemistry studies revealed that the growth defects were filled with cartilaginous tissue, which has a similar structure as natural epiphyseal plate . On the left side (control), the epiphyseal defect was filled with bone bridge and epiphyseal plate growth was arrestted. Conclusions Epiphyseal cartilage tissue engineering prevents bone bridge generation and also has some growth potential. It may be a suitable treatment materials for repairing the epiphyseal plate defect