中华劳动卫生职业病杂志
中華勞動衛生職業病雜誌
중화노동위생직업병잡지
CHINESE JOURNAL OF INDUSTRIAL HYGIENE AND OCCUPATIONAL DISEASES
2010年
1期
12-17
,共6页
冀芳%朱守民%王爱红%曲亚斌%顾寿永%朱人%柴尚建%李俊%夏昭林
冀芳%硃守民%王愛紅%麯亞斌%顧壽永%硃人%柴尚建%李俊%夏昭林
기방%주수민%왕애홍%곡아빈%고수영%주인%시상건%리준%하소림
氯乙烯%DNA损伤%多态性%单核苷酸%疾病遗传易感性
氯乙烯%DNA損傷%多態性%單覈苷痠%疾病遺傳易感性
록을희%DNA손상%다태성%단핵감산%질병유전역감성
Vinyl chloride%DNA damage%Polymorphism,Single nucleotide%Genetic predisposition to disease
目的 探讨氯乙烯(VCM)致DNA损伤与DNA修复基因和代谢酶基因多态性的关系.方法 彗星试验检测DNA损伤,并按DNA损伤情况将接触VCM的研究对象分为DNA损伤组(75人),采用病例对照设计方法选择对照组(75人),用聚合酶链反应-限制性酶切片段多态性技术(PCR-RFLP)和PCR测XRCC1(Arg194Trp,Arg280His和Arg399Gln)、XPD(Ile199Met,Asp312Asn和Lys751Gln)和代谢酶(CYP2E1、GSTT1和GSTM1)基因多态性.结果 单因素分析结果表明,CYP2E1c1c2、c2c2和XPD 751 Lys/Gln、Gln/Gln基因犁携带者DNA损伤风险明显增高,而XRCC1 399 Arg/Gln、Gln/Gln基因型携带者DNA损伤风险明显降低,差异均有统计学意义(P<0.05,P<0.01).logistic回归分析发现,XRCC1 194、XRCC1 399、XPD751和CYP2E1多态与DNA损伤有关.累积接触剂量分析表明,具XRCC1 399Arg/Gln、Gln/Gln基因型个体即使在累积接触剂量高的情况下DNA损伤发生率仍明显降低(OR:0.35,95%CI:0.12～1.01);具CYP2E1 c1/c2、c2/c2基因型个体,无论累积接触剂量高低,其DNA损伤发生率均明显高于对照(OR:2.57,95%CI:1.01～6.59和OR:2.57,95%CI:0.99～6.87).结论 VCM累积接触剂量和CYP2E1、XRCC1 194、XRCC1 399及XPD751基因型与VCM诱导的DNA损伤有关.
目的 探討氯乙烯(VCM)緻DNA損傷與DNA脩複基因和代謝酶基因多態性的關繫.方法 彗星試驗檢測DNA損傷,併按DNA損傷情況將接觸VCM的研究對象分為DNA損傷組(75人),採用病例對照設計方法選擇對照組(75人),用聚閤酶鏈反應-限製性酶切片段多態性技術(PCR-RFLP)和PCR測XRCC1(Arg194Trp,Arg280His和Arg399Gln)、XPD(Ile199Met,Asp312Asn和Lys751Gln)和代謝酶(CYP2E1、GSTT1和GSTM1)基因多態性.結果 單因素分析結果錶明,CYP2E1c1c2、c2c2和XPD 751 Lys/Gln、Gln/Gln基因犛攜帶者DNA損傷風險明顯增高,而XRCC1 399 Arg/Gln、Gln/Gln基因型攜帶者DNA損傷風險明顯降低,差異均有統計學意義(P<0.05,P<0.01).logistic迴歸分析髮現,XRCC1 194、XRCC1 399、XPD751和CYP2E1多態與DNA損傷有關.纍積接觸劑量分析錶明,具XRCC1 399Arg/Gln、Gln/Gln基因型箇體即使在纍積接觸劑量高的情況下DNA損傷髮生率仍明顯降低(OR:0.35,95%CI:0.12～1.01);具CYP2E1 c1/c2、c2/c2基因型箇體,無論纍積接觸劑量高低,其DNA損傷髮生率均明顯高于對照(OR:2.57,95%CI:1.01～6.59和OR:2.57,95%CI:0.99～6.87).結論 VCM纍積接觸劑量和CYP2E1、XRCC1 194、XRCC1 399及XPD751基因型與VCM誘導的DNA損傷有關.
목적 탐토록을희(VCM)치DNA손상여DNA수복기인화대사매기인다태성적관계.방법 혜성시험검측DNA손상,병안DNA손상정황장접촉VCM적연구대상분위DNA손상조(75인),채용병례대조설계방법선택대조조(75인),용취합매련반응-한제성매절편단다태성기술(PCR-RFLP)화PCR측XRCC1(Arg194Trp,Arg280His화Arg399Gln)、XPD(Ile199Met,Asp312Asn화Lys751Gln)화대사매(CYP2E1、GSTT1화GSTM1)기인다태성.결과 단인소분석결과표명,CYP2E1c1c2、c2c2화XPD 751 Lys/Gln、Gln/Gln기인리휴대자DNA손상풍험명현증고,이XRCC1 399 Arg/Gln、Gln/Gln기인형휴대자DNA손상풍험명현강저,차이균유통계학의의(P<0.05,P<0.01).logistic회귀분석발현,XRCC1 194、XRCC1 399、XPD751화CYP2E1다태여DNA손상유관.루적접촉제량분석표명,구XRCC1 399Arg/Gln、Gln/Gln기인형개체즉사재루적접촉제량고적정황하DNA손상발생솔잉명현강저(OR:0.35,95%CI:0.12～1.01);구CYP2E1 c1/c2、c2/c2기인형개체,무론루적접촉제량고저,기DNA손상발생솔균명현고우대조(OR:2.57,95%CI:1.01～6.59화OR:2.57,95%CI:0.99～6.87).결론 VCM루적접촉제량화CYP2E1、XRCC1 194、XRCC1 399급XPD751기인형여VCM유도적DNA손상유관.
Objective To explore the association between DNA damage induced by vinyl chloride monomer (VCM) and polymorphisms of DNA repair genes and xenobiotic metabolism genes of VCM. Methods Comet assay was employed to detect DNA damage. Based on the status of DNA damage, the VCM exposure workers were divided into two groups: DNA damage group (75) and control group (75). Case-control design was used to investigate the association between the genetic polymorphisms and DNA damage induced by VCM. Genotypes of XRCCI (Arg194Trp, Arg280His and Arg399Gln), XPD (Ile 199Met, Asp312Ash and Lys751 Gln) and CYP2E1 were identified by the PCR-RFLP. PCR assay was used to detect positive and null genotype of GSTT1 and GSTM1. Results Univariate analysis showed that the CYP2E1 c1c2/c2c2 and XPD751 Lys/Gln and Gln/Gln genotypos were significantly associated with the increased levels of DNA damage, XRCC1 339 Arg/Gln and Gln/Gln gnnotypes were significantly associated with the decreased levels of DNA damage(P<0.01, P<0.05, respectively). Logistic regression analysis showed that there was significant association between the genotypes of XRCC1 194,XRCC1 399, XPD 751, CYP2E1 and DNA damages. A prominent risk decreasing of DNA damage was observed for those individuals possessing XRCC1 399Arg/Gln +Gln/Gln genotypes (OR: 0.35,95% CI:0.12～1.01 ,respectively); The results also showed that there were significant associations be-tween CYP2E1 c1c2/c2c2 and DNA damage both in high and low VCM-exposed groups (OR:2.57,95% CI: 1.01～6.59 and OR:2.57,95% CI:0.99～6.87). Conclusion Cumulative exposure dose. and genotypes of XR-CC1 194, XRCC1 399, XPD 751 and CYP2E1 my modulate the DNA damage induced by VCM exposure.
