CAJ | 학술논문

目的重复阻断人一侧上臂血液供应,监测循环血中腺苷含量变化,探讨局部缺血预适应对全身的保护效应.方法选取5例健康成年自愿者制备人体重复上臂压迫模型,用袖带压迫肱动脉,加压至200 mm Hg(1 mm Hg=0.133 kPa),持续5 min、间隔5 min,重复5次,采用高效液相色谱(HPLC)的方法,观察压迫前后血浆中腺苷(ADO)含量的变化,并记录受试者的主观感受.结果 HPLC结果显示,重复上臂压迫前后ADO含量呈现规律性变化,在5次压迫结束后ADO含量升高,在压迫后15、30 min降低,在60 min达到最高值(1069.622μg/L),达4.44±0.19倍于压迫前(P<0.01).在压迫后24 h血浆中ADO含量依然保持高水平.循环血腺苷含量升高的同时,受试者缺血侧上臂的不适感却明显降低(P<0.01).结论上臂血供重复阻断中,血浆腺苷含量显著提升,缺血肢对缺血的耐受显著增强,提示重复阻断一侧上臂血供可激发全身性远程异位低氧预适应的保护物质.
목적 중복조단인일측상비혈액공응,감측순배혈중선감함량변화,탐토국부결혈예괄응대전신적보호효응.방법 선취5례건강성년자원자제비인체중복상비압박모형,용수대압박굉동맥,가압지200 mm Hg(1 mm Hg=0.133 kPa),지속5 min、간격5 min,중복5차,채용고효액상색보(HPLC)적방법,관찰압박전후혈장중선감(ADO)함량적변화,병기록수시자적주관감수.결과 HPLC결과 현시,중복상비압박전후ADO함량정현규률성변화,재5차압박결속후ADO함량승고,재압박후15、30 min강저,재60 min체도최고치(1069.622μg/L),체4.44±0.19배우압박전(P<0.01).재압박후24 h혈장중ADO함량의연보지고수평.순배혈선감함량승고적동시,수시자결혈측상비적불괄감각명현강저(P<0.01).결론 상비혈공중복조단중,혈장선감함량현저제승,결혈지대결혈적내수현저증강,제시중복조단일측상비혈공가격발전신성원정이위저양예괄응적보호물질.
Objective To establish a simple model of brief upper arm compression in humans, detecting the changes of concentration of serum adenosine (ADO) ,to study the general protection of local ischemic preconditioning. Methods The study was performed on 5 healthy adult volunteers. Isehemie state was made on the upper ann by inflating a blood pressure cuff to 200 mmHg for five 5 min periods, separated by 5 min of reperfusion. The feelings of the volunteers were documented during the compressions. The venous blood at different time points was obtained. HPLC was used to measure the concentration of ADO in the circulating blood. Results ADO was regularly increased ,and reached the peak value of 1069. 622 μg/L at p-60 rain (P < 0.01) and sustained the high level in p-24h group. Meanwhile the uncomfortable feeling of volunteers decreased. The results of the study show that remote ischemic preconditioning can increase the tolerance of the arm to ischemic injury. This effect may contribute to the protective effect of isehemic preconditioning against ischemia-perfusion injury.