CAJ | 학술논문

目的观察急性高眼压模型兔眼不同眼压状态下视神经轴浆运输的改变。方法成年新西兰大白兔24只，分为眼压20、30、40 mm Hg(1 mm Hg=0.133 kPa)组和眼压为10～15 mm Hg的对照组，每组均为6只兔。采用前房穿刺灌注法联合压力持续监测法建立急性高眼压模型。实验开始时，兔眼玻璃体腔中注射罗丹明异硫氰酸(RITC)标记轴浆运输。持续3h高眼压后，过量麻醉处死兔后取下视神经。荧光显微镜下观察视神经轴浆运输情况的改变。采用德国Leica公司Q500IW图像分析软件对RITC进行灰度定量分析，并对各眼压组平均灰度值和筛板前、筛板区、筛板后350 μm区域灰度值进行统计学分析处理。结果 RITC在视神经中心呈顺行标记染色。不同眼压组轴浆运输情况不同，随着眼压升高，轴浆运输能力减弱，差异有统计学意义(F=159.3，P＜0.05)。筛板前区，各眼压组间灰度值比较，差异无统计学意义(F=0.2545，P＞0.05)。40 mm Hg组灰度值与对照组灰度值比较，在筛板区（t=5.684）和筛板后350 μm区域（t=5.124）差异均有统计学意义(P＜0.05);20、30 mm Hg组灰度值与对照组灰度值比较，差异无统计学意义(t=1.747，P＞0.05)。结论眼压40 mm Hg持续3h将导致视神经轴浆运输改变，轴浆运输障碍部位以筛板区及以后的区域为主。
목적 관찰급성고안압모형토안불동안압상태하시신경축장운수적개변。방법 성년신서란대백토24지，분위안압20、30、40 mm Hg(1 mm Hg=0.133 kPa)조화안압위10～15 mm Hg적대조조，매조균위6지토。채용전방천자관주법연합압력지속감측법건립급성고안압모형。실험개시시，토안파리체강중주사라단명이류청산(RITC)표기축장운수。지속3h고안압후，과량마취처사토후취하시신경。형광현미경하관찰시신경축장운수정황적개변。채용덕국Leica공사Q500IW도상분석연건대RITC진행회도정량분석，병대각안압조평균회도치화사판전、사판구、사판후350 μm구역회도치진행통계학분석처리。결과 RITC재시신경중심정순행표기염색。불동안압조축장운수정황불동，수착안압승고，축장운수능력감약，차이유통계학의의(F=159.3，P＜0.05)。사판전구，각안압조간회도치비교，차이무통계학의의(F=0.2545，P＞0.05)。40 mm Hg조회도치여대조조회도치비교，재사판구（t=5.684）화사판후350 μm구역（t=5.124）차이균유통계학의의(P＜0.05);20、30 mm Hg조회도치여대조조회도치비교，차이무통계학의의(t=1.747，P＞0.05)。결론 안압40 mm Hg지속3h장도치시신경축장운수개변，축장운수장애부위이사판구급이후적구역위주。
ObjectivesTo observe the changes of axonal transport in the rabbit optic nerve under acute ocular hypertensions. Methods24 adult healthy New Zealand white rabbits were divided into 4groups according to the intraocular pressure (IOP), with 6 rabbits in each group. There are 3 experimental groups with an IOP of 20, 30, 40 mm Hg (1 mm Hg=0. 133 kPa). respectively, and 1 control group with an IOP of 10-15 mm Hg. Two 25-gauge cannulas were inserted into each rabbit's anterior chamber to create the model of acute ocular hypertension. At the beginning of the experiment, rhodamine-β-isothiocyanate (RITC) was injected into the vitreous of each eye to label axonal transport. After 3 hours of high intraocular pressure, rabbits were sacrificed with anesthetic overdose. The retina and the optic nerve were then carefully exposed. Fluorescent microscopy was used for quantitative measurements of the changes of optic nerve axonal transport. Statistically analyze the average grey level in different groups and sites by Leica RITC Q500IW image analysis software. ResultsRITC, a fluorescent tracer, was transported in the anterograde direction by axonal transport. With the increasing of the intraocular pressure, the distance of the axonal transport was declined (F=159.3, P＜0. 05). The difference of the grey level in the pre-laminar region of 20, 30, 40 mm Hg group was not statistically significant (F=0. 2545,P＞0. 05). Compared the grey level of 40 mm Hg group with control group, the differences in lamina cribrosa(t=5. 684)and the proximal 350 μm of the post-laminar (t =5. 124) were statistically significant (P＜ 0. 05). Compared the grey level of 20, 30 mm Hg group with control group, the difference was not statistically significant (t =1. 747, P＞0. 05). Conclusion 40 mmHg intraocular pressure lasts for 3 hours can reduce axonal transport in the lamina cribrosa and post laminar of optic nerve.