CAJ | 학술논문

目的探讨迷迭香复合精油对长期慢性脑缺血导致的血管性痴呆(VD)大鼠学习记忆功能及海马CA1区胆碱乙酰转移酶(ChAT)的影响.方法雄性2月龄SD大鼠60只,按随机数字表法分为正常对照组(10只)、假手术组(10只),余40只大鼠采用改良双侧颈总动脉永久结扎法(2-VO)法制作大鼠VD模型,将造模48 h后成活的30只大鼠再次按随机数字表法分为模型组、迷迭香复合精油组和依达拉奉组,每组10只大鼠.迷迭香复合精油组给予吸嗅迷迭香复合精油,每日2次精油吸嗅,每次30 min;依达拉奉组给予腹腔注射依达拉奉注射液,剂量3 mg/kg体重,各持续65 d.通过水迷宫试验来评价各组大鼠学习记忆能力,并采用免疫组化技术观察海马CA1区组织形态学和ChAT表达的变化.结果水迷宫试验结果显示,模型组大鼠学习记忆能力明显差于假手术组和正常对照组(P＜0.01),而迷迭香复合精油组和依达拉奉组则优于模型组(P＜0.01).模型组大鼠大脑海马区及纹状体发生明显的病理形态学改变,迷迭香复合精油组和依达拉奉组与模型组比较,海马区神经细胞损害较轻(P＜0.01);迷迭香复合精油组大鼠海马CA1区ChAT的阳性单位数较模型组显著增加,且差异有统计学意义(P＜0.01).结论迷迭香复合精油通过嗅觉途径能够改善慢性长期脑缺血所致的VD大鼠学习记忆功能,减轻海马区神经细胞损害;乙酰胆碱转移酶阳性细胞表达明显增加,对VD有治疗作用.
목적 탐토미질향복합정유대장기만성뇌결혈도치적혈관성치태(VD)대서학습기억공능급해마CA1구담감을선전이매(ChAT)적영향.방법 웅성2월령SD대서60지,안수궤수자표법분위정상대조조(10지)、가수술조(10지),여40지대서채용개량쌍측경총동맥영구결찰법(2-VO)법제작대서VD모형,장조모48 h후성활적30지대서재차안수궤수자표법분위모형조、미질향복합정유조화의체랍봉조,매조10지대서.미질향복합정유조급여흡후미질향복합정유,매일2차정유흡후,매차30 min;의체랍봉조급여복강주사의체랍봉주사액,제량3 mg/kg체중,각지속65 d.통과수미궁시험래평개각조대서학습기억능력,병채용면역조화기술관찰해마CA1구조직형태학화ChAT표체적변화.결과 수미궁시험결과현시,모형조대서학습기억능력명현차우가수술조화정상대조조(P＜0.01),이미질향복합정유조화의체랍봉조칙우우모형조(P＜0.01).모형조대서대뇌해마구급문상체발생명현적병리형태학개변,미질향복합정유조화의체랍봉조여모형조비교,해마구신경세포손해교경(P＜0.01);미질향복합정유조대서해마CA1구ChAT적양성단위수교모형조현저증가,차차이유통계학의의(P＜0.01).결론 미질향복합정유통과후각도경능구개선만성장기뇌결혈소치적VD대서학습기억공능,감경해마구신경세포손해;을선담감전이매양성세포표체명현증가,대VD유치료작용.
Objective To determine the effect of inhaling rosemary compound essential oil on learning and memory and the expression of choline acetyl transferase (ChAT) in the CA1 region of the hippocampus in rats with vascular dementia (VD).Methods A modified 2-VO method was used to induce permanent brain hypoperfusion,and thus VD,in 60 male SD rats aged 2 months.They were then randomly divided into a normal control group (n =10),a sham-operation group (n =10),a model group (n =10),a rosemary compound essential oil group (n =10) and an edaravone group (n =10).The rosemary compound essential oil group inhaled a compound of the essential oils of rosemary,eugenol and peppermint once a day; the edaravone group was given a daily injection of edaravone injecta.Both the interventions lasted for 65 days.A water maze was used to test their learning and memory abilities.Morphological damage in the CA1 region of the hippocampus and the expression of ChAT were also observed.Results The learning and memory abilities of the model group were significantly weaker compared with the sham-operated group and the normal controls.The rats in the rosemary compound essential oil and edaravone groups were obviously better than those in the model group,but there was no significant difference between rosemary compound essential oil group and the edaravone group.The morphological damage observed in the hippocampus and striatum of the model group was significantly greater,on average,than that observed in the rosemary compound essential oil group and the edaravone group.The average number of ChAT positive units in the CA1 region of the hippocampus was significantly greater in both the rosemary compound essential oil group and the edaravone group compared with the model group,but there was no significant difference between the rosemary compound essential oil group and the edaravone group.Conclusions Inhaling rosemary compound essential oil can improve the learning and memory abilities of rats with VD caused by permanent chronic brain hypoperfusion.It decreases the damage to the neurons of the hippocampus,increases the expression of ChAT,and shows a therapeutic effect on VD,at least in rats.