昆虫学报
昆蟲學報
곤충학보
ACTA ENTOMOLOGICA SINICA
2009年
10期
1083-1089
,共7页
何芳%姜爱兰%李神斌%吴运梅%王国秀
何芳%薑愛蘭%李神斌%吳運梅%王國秀
하방%강애란%리신빈%오운매%왕국수
中华卵索线虫%昆虫病原线虫%线粒体基因组%多态性
中華卵索線蟲%昆蟲病原線蟲%線粒體基因組%多態性
중화란색선충%곤충병원선충%선립체기인조%다태성
Ovomermis sinensis%entomogenous nematodes%mitochondrial genome%polymorphism
为完善昆虫病原索科线虫线粒体基因组全序列数据库,更系统地研究其基因组特征和系统演化规律,进而为发挥该线虫生防潜力打下基础,我们开展了中华卵索线虫Ovomermis sinensis线粒体全基因组的研究.该研究通过线粒体基因组滚环复制及酶切图谱,揭示了中华卵索线虫线粒体基因组具有种内遗传多态性,即群体中单体线虫具有独特的酶切条带,且条带累加之和变化范围较大.为16.5~24.5 kb.为进一步了解线粒体基因组多态性特征及产生的分子机制,采用两步长PCR方法对2条代表性成虫线粒体基冈组进行了测序及拼接,得其全长分别为18 864和16 777 bp.对这2条序列的比对表明,线粒体基因组中位于ND2和ND4之间的可变区域,不仅基因排列顺序不同,且存在ND3基因重复现象,这是导致中华卵索线虫线粒体基因组呈现多态性的主要原因.通过对以上研究结果的分析及与GenBank中已有的6种索科线虫线粒体基因组序列进行比对,概括出其线粒体基因组基本特点:①线粒体基因排列顺序各不相同;②部分线虫线粒体基因存在重复现象,且重复次数不同;③线粒体基因组大小存在很大差异.
為完善昆蟲病原索科線蟲線粒體基因組全序列數據庫,更繫統地研究其基因組特徵和繫統縯化規律,進而為髮揮該線蟲生防潛力打下基礎,我們開展瞭中華卵索線蟲Ovomermis sinensis線粒體全基因組的研究.該研究通過線粒體基因組滾環複製及酶切圖譜,揭示瞭中華卵索線蟲線粒體基因組具有種內遺傳多態性,即群體中單體線蟲具有獨特的酶切條帶,且條帶纍加之和變化範圍較大.為16.5~24.5 kb.為進一步瞭解線粒體基因組多態性特徵及產生的分子機製,採用兩步長PCR方法對2條代錶性成蟲線粒體基岡組進行瞭測序及拼接,得其全長分彆為18 864和16 777 bp.對這2條序列的比對錶明,線粒體基因組中位于ND2和ND4之間的可變區域,不僅基因排列順序不同,且存在ND3基因重複現象,這是導緻中華卵索線蟲線粒體基因組呈現多態性的主要原因.通過對以上研究結果的分析及與GenBank中已有的6種索科線蟲線粒體基因組序列進行比對,概括齣其線粒體基因組基本特點:①線粒體基因排列順序各不相同;②部分線蟲線粒體基因存在重複現象,且重複次數不同;③線粒體基因組大小存在很大差異.
위완선곤충병원색과선충선립체기인조전서렬수거고,경계통지연구기기인조특정화계통연화규률,진이위발휘해선충생방잠력타하기출,아문개전료중화란색선충Ovomermis sinensis선립체전기인조적연구.해연구통과선립체기인조곤배복제급매절도보,게시료중화란색선충선립체기인조구유충내유전다태성,즉군체중단체선충구유독특적매절조대,차조대루가지화변화범위교대.위16.5~24.5 kb.위진일보료해선립체기인조다태성특정급산생적분자궤제,채용량보장PCR방법대2조대표성성충선립체기강조진행료측서급병접,득기전장분별위18 864화16 777 bp.대저2조서렬적비대표명,선립체기인조중위우ND2화ND4지간적가변구역,불부기인배렬순서불동,차존재ND3기인중복현상,저시도치중화란색선충선립체기인조정현다태성적주요원인.통과대이상연구결과적분석급여GenBank중이유적6충색과선충선립체기인조서렬진행비대,개괄출기선립체기인조기본특점:①선립체기인배렬순서각불상동;②부분선충선립체기인존재중복현상,차중복차수불동;③선립체기인조대소존재흔대차이.
To augment the databases of Mermithidae mitochondrial genome, to study the mitochondrial genome characteristics and phylogenies of the nematodes, and to provide the theoretical information for the biological control, we carried out the research on the entomopathogenic nematode Ovomermis sinensis. The characterization of mitochondrial genomes from individual O. sinensis nematodes showed that numerous mtDNA haplotypes, ranging in size from 16.5 to 24.5 kb. To understand the molecular basis of this hypervariation, complete nucleotide sequences of two 0. sinensis mtDNA haplotypes were determined by two-step long-PCR, with the length of 18 864 and 16 777 bp, respectively. Our data revealed that 0. sinensis mtDNA can be divided into a constant region and a hypervariable region. The hypervariable segment, residing between ND2 and ND4, contributes exclusively to 0. sinensis mtDNA size variation. Within this region, mtDNA coding genes and putative nonfunctional sequences are duplicated and rearranged to varying extents. Based on the above analysis and the comparison of the mitochondrial genomes of 0. sinensis and other six species of Mermithidae, the following characteristics of the mitochondrial genomes of Mermithidae were summarized: firstly, the gene orders of mitochondrial genomes in Mermithidae are different; secondly, the frequency of gene duplication is variable among some species; and thirdly, the sizes of complete mitochondrial genomes are different.