CAJ | 학술논문

对刺龙芽回枯病病菌生物学特性的初步研究结果表明,PDA培养基适宜于该病菌生长,寄主煎汁培养基有利于分生孢子产生;葡萄糖、淀粉、蛋白胨、牛肉膏是该病菌生长的适宜碳源和氮源,酵母液、谷氨酸可保持其很高的生物量,但却不利于菌丝的平面生长,果糖、尿素、丙氨酸和硝酸铵有利于分生孢子的产生,丙氨酸和硝酸铵对促进菌丝的营养生长和生殖生长具有双重作用,缺碳和氮条件不利菌丝生长和繁殖;病菌菌丝在其易于利用的营养条件下呈白色且生长稠密,反之颜色变深,生长变稀.菌丝最适宜生长温度25～30℃,分生孢子最佳萌发温度为30℃;菌丝在60℃保存20min或分生孢子在55℃保存20min条件下可导致死亡.光照条件对菌丝生长和分生孢子萌发没有影响,对分生孢子的产生影响较大.菌丝生长的最佳pH值为7.0,偏酸或偏碱环境有利于分生孢子产生,中性环境不利于分生孢子产生,分生孢子萌发的最佳pH值为6.0～7.0.
대자룡아회고병병균생물학특성적초보연구결과표명,PDA배양기괄의우해병균생장,기주전즙배양기유리우분생포자산생;포도당、정분、단백동、우육고시해병균생장적괄의탄원화담원,효모액、곡안산가보지기흔고적생물량,단각불리우균사적평면생장,과당、뇨소、병안산화초산안유리우분생포자적산생,병안산화초산안대촉진균사적영양생장화생식생장구유쌍중작용,결탄화담조건불리균사생장화번식;병균균사재기역우이용적영양조건하정백색차생장주밀,반지안색변심,생장변희.균사최괄의생장온도25～30℃,분생포자최가맹발온도위30℃;균사재60℃보존20min혹분생포자재55℃보존20min조건하가도치사망.광조조건대균사생장화분생포자맹발몰유영향,대분생포자적산생영향교대.균사생장적최가pH치위7.0,편산혹편감배경유리우분생포자산생,중성배경불리우분생포자산생,분생포자맹발적최가pH치위6.0～7.0.
An experiment was conducted to study the biological properties of the pathogen of Fusarium larvarum causing wilt disease of Arlia elata. Results show that PDA culture medium is optimal for the pathogen growth and the culture medium with extract from root and stem of host can improve the conidium generation; the optimal carbon source and nitrogen source are glucose, starch, peptone and, extract of beef, respectively, and the yeast extract and glutamic acid can maintain higher biomass, but they are not beneficial to the hyphal growth on horizontal plane. The conidia are more than the others in the culture medium containing either of fructose, urea, lactamic acid or ammonium nitrate, in which lactamic acid and ammonium nitrate can not only improve the pathogen growth, but also generate conidia count. The hypha and conidia can not develop well without carbon source and nitrogen source. The hypha are white and thick in the optimal culture medium, on the contrary, the hypha gradually turn into brown and thin. The optimal temperatures for hyphal growth and conidium germination are 25 to 35 degrees C and 30 degrees C, respectively. The hypha and the conidia would be dead at 60 degrees C and 55 degrees C for 20 minutes, respectively. Light intensity has no effect on both hyphal growth and conidium germination, but has a significant effect on conidia generation. The optimal pH value for hyphal growth is 7. More conidia generate at pH less than 7 or pH greater than 7, but the hypha grow well under neutral condition. The optimal pH value for conidia germination is 6 to 7.