时珍国医国药
時珍國醫國藥
시진국의국약
LISHIZHEN MEDICINE AND MATERIA MEDICA RESEARCH
2010年
2期
352-353
,共2页
谢彩侠%史会齐%高山林%胡亚楠%白雨%白雁
謝綵俠%史會齊%高山林%鬍亞楠%白雨%白雁
사채협%사회제%고산림%호아남%백우%백안
盾叶薯蓣%四倍体%抗氧化酶活性%薯蓣皂苷元
盾葉藷蕷%四倍體%抗氧化酶活性%藷蕷皂苷元
순협서여%사배체%항양화매활성%서여조감원
D. zingibiernsisC.H.Wright.%Antotetraploid%Autioxidant enzyme activities%Diosgenin
目的 提高盾叶薯蓣药材的品质.方法 通过组织培养,对诱导获得的盾叶薯蓣四倍体株系,进行了试管苗抗氧化酶活性及薯蓣皂苷元含量分析.结果 大部分四倍体株系APX活性、SOD和POD比活力高于或相近于二倍体株系.经诱导获得的四倍体株系试管苗与对照相比薯蓣皂苷元含量有较大差异,大部分四倍体株系薯蓣皂苷元含量都超过了二倍体株系.结论 经诱导获得的四倍体株系在抗氧化酶活性及有效成分含量方面与二倍体表现出明显差异,说明多倍体诱导可以作为盾叶薯蓣优良品种选育的途径.
目的 提高盾葉藷蕷藥材的品質.方法 通過組織培養,對誘導穫得的盾葉藷蕷四倍體株繫,進行瞭試管苗抗氧化酶活性及藷蕷皂苷元含量分析.結果 大部分四倍體株繫APX活性、SOD和POD比活力高于或相近于二倍體株繫.經誘導穫得的四倍體株繫試管苗與對照相比藷蕷皂苷元含量有較大差異,大部分四倍體株繫藷蕷皂苷元含量都超過瞭二倍體株繫.結論 經誘導穫得的四倍體株繫在抗氧化酶活性及有效成分含量方麵與二倍體錶現齣明顯差異,說明多倍體誘導可以作為盾葉藷蕷優良品種選育的途徑.
목적 제고순협서여약재적품질.방법 통과조직배양,대유도획득적순협서여사배체주계,진행료시관묘항양화매활성급서여조감원함량분석.결과 대부분사배체주계APX활성、SOD화POD비활력고우혹상근우이배체주계.경유도획득적사배체주계시관묘여대조상비서여조감원함량유교대차이,대부분사배체주계서여조감원함량도초과료이배체주계.결론 경유도획득적사배체주계재항양화매활성급유효성분함량방면여이배체표현출명현차이,설명다배체유도가이작위순협서여우량품충선육적도경.
Objective To improve the yield and quality of D. zingibiernsis C.H.Wright . Methods The research project of polyploidy breeding by tissue culture was done. The antioxidant enzyme activities and the diosgenin were analyzed in polyploidy lines. Results The results showed that SOD and POD activities in most of autotetraploid lines were not lower than those of diploid line, furthermore,the content of diosgenin in most induced plantlets was higher than that in control. Conclusion There was difference between autotetraploid and control lines in diosgenin content and antioxidant enzyme activities,therefore,inducing autotetraploid can be effective way to breeding the superior varieties.
