中国病理生理杂志
中國病理生理雜誌
중국병리생리잡지
CHINESE JOURNAL OF PATHOPHYSIOLOGY
2010年
3期
435-439
,共5页
王莹萍%布会敏%王妹玲%杨长瑛%周兆年%张翼
王瑩萍%佈會敏%王妹玲%楊長瑛%週兆年%張翼
왕형평%포회민%왕매령%양장영%주조년%장익
慢性间歇性低压低氧%受体,肾上腺素能%窦房结%动作电位%兔
慢性間歇性低壓低氧%受體,腎上腺素能%竇房結%動作電位%兔
만성간헐성저압저양%수체,신상선소능%두방결%동작전위%토
Chronic intermittent hypobaric hypoxia%Receptors,adrenergic%Sinoatrial node%Action potentials%Rabbits
目的:通过细胞内记录方法研究慢性间歇性低压低氧(CIHH)对家兔窦房结肾上腺素能受体反应性的影响.方法:新西兰大白兔随机分为对照组(Con)、CIHH 14 d组(CIHH 14)、CIHH 28 d组(CIHH 28)3组.CIHH组动物置于低压氧舱,分别接受14、28 d模拟5 000米高原的低压低氧处理(6 h/d).制备离体窦房结标本、描记窦房结自律细胞的生物电活动.通过累加给药法,观察不同浓度的β-肾上腺素能受体激动剂异丙肾上腺素(ISO) (0.01、0.1、1 μmol/L)和α_1肾上腺素能受体激动剂苯肾上腺素(PE) (0.01、0.1、1 μmol/L)对家兔窦房结自律细胞动作电位的影响.结果:(1) CIHH对基础状态下窦房结细胞动作电位各参数无明显影响;(2)ISO浓度依赖性增加窦房结细胞动作电位0期最大除极速率(V_(max))、动作电位幅值(APA)、放电频率(RPF)、舒张期自动去极化速率(VDD),对其它参数无明显影响;(3)CIHH处理动物窦房结细胞对ISO的反应性降低;最大浓度ISO(1 μmol/L)下,CIHH14和CIHH28组的VDD、RPF、APA和V_(max)的增加明显小于对照组(P<0.05);(4)α_1肾上腺素能受体激动剂PE(0.01、0.1、1 μmol/L) 对窦房结细胞动作电位各项参数均无影响.结论:CIHH降低家兔窦房结β肾上腺素能受体反应性,对α_1肾上腺素能受体反应性无影响.
目的:通過細胞內記錄方法研究慢性間歇性低壓低氧(CIHH)對傢兔竇房結腎上腺素能受體反應性的影響.方法:新西蘭大白兔隨機分為對照組(Con)、CIHH 14 d組(CIHH 14)、CIHH 28 d組(CIHH 28)3組.CIHH組動物置于低壓氧艙,分彆接受14、28 d模擬5 000米高原的低壓低氧處理(6 h/d).製備離體竇房結標本、描記竇房結自律細胞的生物電活動.通過纍加給藥法,觀察不同濃度的β-腎上腺素能受體激動劑異丙腎上腺素(ISO) (0.01、0.1、1 μmol/L)和α_1腎上腺素能受體激動劑苯腎上腺素(PE) (0.01、0.1、1 μmol/L)對傢兔竇房結自律細胞動作電位的影響.結果:(1) CIHH對基礎狀態下竇房結細胞動作電位各參數無明顯影響;(2)ISO濃度依賴性增加竇房結細胞動作電位0期最大除極速率(V_(max))、動作電位幅值(APA)、放電頻率(RPF)、舒張期自動去極化速率(VDD),對其它參數無明顯影響;(3)CIHH處理動物竇房結細胞對ISO的反應性降低;最大濃度ISO(1 μmol/L)下,CIHH14和CIHH28組的VDD、RPF、APA和V_(max)的增加明顯小于對照組(P<0.05);(4)α_1腎上腺素能受體激動劑PE(0.01、0.1、1 μmol/L) 對竇房結細胞動作電位各項參數均無影響.結論:CIHH降低傢兔竇房結β腎上腺素能受體反應性,對α_1腎上腺素能受體反應性無影響.
목적:통과세포내기록방법연구만성간헐성저압저양(CIHH)대가토두방결신상선소능수체반응성적영향.방법:신서란대백토수궤분위대조조(Con)、CIHH 14 d조(CIHH 14)、CIHH 28 d조(CIHH 28)3조.CIHH조동물치우저압양창,분별접수14、28 d모의5 000미고원적저압저양처리(6 h/d).제비리체두방결표본、묘기두방결자률세포적생물전활동.통과루가급약법,관찰불동농도적β-신상선소능수체격동제이병신상선소(ISO) (0.01、0.1、1 μmol/L)화α_1신상선소능수체격동제분신상선소(PE) (0.01、0.1、1 μmol/L)대가토두방결자률세포동작전위적영향.결과:(1) CIHH대기출상태하두방결세포동작전위각삼수무명현영향;(2)ISO농도의뢰성증가두방결세포동작전위0기최대제겁속솔(V_(max))、동작전위폭치(APA)、방전빈솔(RPF)、서장기자동거겁화속솔(VDD),대기타삼수무명현영향;(3)CIHH처리동물두방결세포대ISO적반응성강저;최대농도ISO(1 μmol/L)하,CIHH14화CIHH28조적VDD、RPF、APA화V_(max)적증가명현소우대조조(P<0.05);(4)α_1신상선소능수체격동제PE(0.01、0.1、1 μmol/L) 대두방결세포동작전위각항삼수균무영향.결론:CIHH강저가토두방결β신상선소능수체반응성,대α_1신상선소능수체반응성무영향.
AIM: To investigate the effect of chronic intermittent hypobaric hypoxia (CIHH) on the activity of adrenergic receptor(AR) in rabbit sino-atrial node (SAN) by intracellular recording.METHODS: New Zealand rabbits were randomly divided into three groups: control group (Con), 14 d CIHH treatment group (CIHH14) and 28 d CIHH treatment group (CIHH28). The CIHH rabbits were exposed to a simulated 5 000 m (oxygen 11.1%) hypobaric hypoxia in hypoxic chamber for 14 d or 28 d (6 h/d), respectively. SAN preparation was used and the transmembrane action potential was recorded by micropipettes. Isoproterenol hydrochloride (ISO, agonist of β-AR) and phenylephrine (PE, agonist of α_1-AR) at different concentrations (0.01, 0.1 and 1 μmol/L) were applied cumulatively to investigate the electrophysiological effect of the drugs on the rabbit SAN among Con, CIHH14 and CIHH28 groups, respectively. RESULTS: (1) CIHH didnt change the parameters of action potential of SAN recorded under basic condition. (2) ISO changed some parameters of AP significantly in a dose-dependent manner, including increases in the amplitude of AP (APA), maximal rate of depolarization (V_(max)), the velocity of diastolic (phase 4) depolarization (VDD), and rate of pacemaker firing (RPF). (3) The response of AP to ISO in CIHH rabbits was decreased significantly compared to that in Con animals. Under 1 μmol/L of ISO, the increases in VDD, RPF, APA and V_(max) in CIHH animals were smaller than those in Con animals (P<0.05). (4) No effect of PE (0.01, 0.1 and 1 μmol/L) on the parameters of action potential was observed. CONCLUSION: CIHH decreases the reactivity of β-AR, but has no effect on α_1-AR in SAN of rabbit.
