CAJ | 학술논문

目的:探讨poly (I:C)作为病毒类似物对碘过量所致NOD鼠慢性淋巴细胞性甲状腺炎的影响.方法:将32只雌性NOD鼠,随机分为4组,每组8只,分别为:(1) 对照组;(2)碘过量组;(3) poly (I:C)组;(4)碘过量联合poly (I:C)组.作用9周后处死动物:观测体重、甲状腺重量及其解剖形态;采用放射免疫分析法检测血清中甲状腺激素(T_4)水平;HE染色观察甲状腺组织形态变化,TUNEL技术检测甲状腺组织细胞凋亡情况,定量PCR法检测凋亡相关基因(TRAIL、TRAIL-sR1)和炎症相关基因(ICAM-1、CXCL10) mRNA表达.结果:碘过量组较对照组、Poly(I:C)组甲状腺绝对重量及相对重量均明显增加(P<0.01);血清总T_4水平下降(P<0.05);炎症破坏和细胞凋亡明显加强;上调TRAIL、TRAIL-sR1、ICAM-1、CXCL10 mRNA表达(P<0.05).碘过量联合poly (I:C)组较单纯碘过量组甲状腺绝对重量及相对重量均进一步增加;血清总T_4水平显著下降(P<0.05);炎症进一步加重,破坏程度IV级所占比例升至50.0%;凋亡细胞数量明显增加,进一步上调TRAIL、TRAIL-sR1、ICAM-1、CXCL10 mRNA表达(P<0.05).Poly (I:C)组各值与对照组趋势一致(P>0.05).结论:Poly(I:C)加重碘过量所致慢性淋巴细胞性甲状腺炎发病程度,其作用途径与增加淋巴细胞浸润和甲状腺细胞凋亡有关.
목적:탐토poly (I:C)작위병독유사물대전과량소치NOD서만성림파세포성갑상선염적영향.방법:장32지자성NOD서,수궤분위4조,매조8지,분별위:(1) 대조조;(2)전과량조;(3) poly (I:C)조;(4)전과량연합poly (I:C)조.작용9주후처사동물:관측체중、갑상선중량급기해부형태;채용방사면역분석법검측혈청중갑상선격소(T_4)수평;HE염색관찰갑상선조직형태변화,TUNEL기술검측갑상선조직세포조망정황,정량PCR법검측조망상관기인(TRAIL、TRAIL-sR1)화염증상관기인(ICAM-1、CXCL10) mRNA표체.결과:전과량조교대조조、Poly(I:C)조갑상선절대중량급상대중량균명현증가(P<0.01);혈청총T_4수평하강(P<0.05);염증파배화세포조망명현가강;상조TRAIL、TRAIL-sR1、ICAM-1、CXCL10 mRNA표체(P<0.05).전과량연합poly (I:C)조교단순전과량조갑상선절대중량급상대중량균진일보증가;혈청총T_4수평현저하강(P<0.05);염증진일보가중,파배정도IV급소점비례승지50.0%;조망세포수량명현증가,진일보상조TRAIL、TRAIL-sR1、ICAM-1、CXCL10 mRNA표체(P<0.05).Poly (I:C)조각치여대조조추세일치(P>0.05).결론:Poly(I:C)가중전과량소치만성림파세포성갑상선염발병정도,기작용도경여증가림파세포침윤화갑상선세포조망유관.
AIM: To investigate the effects of poly (I:C) as virus mimics on iodine excess-induced chronic lymphocytic thyroiditis in NOD mouse. METHODS: Female, 32 NOD mice were randomly divided into 4 groups: (1) control;(2) high iodine;(3) poly (I:C);(4) high iodine+poly (I:C). Nine weeks after administration, mice were sacrificed. The following parameters were determined: body weight, thyroid weight and anatomic form. Thyroid hormone (T_4) in serum was measured by radioimmunoassay, the thyroid morphology was observed through HE staining, apoptosis was detected by TUNEL, the mRNA expression levels of TRAIL, TRAIL-sR1, ICAM-1 and CXCL10 were determined by the method of real time RT-PCR. RESULTS: Compared to control group and poly (I:C) group, the thyroid absolute weight and relative weight in high iodine group were increased (P<0.01), the level of total T_4 in serum was decreased (P<0.05), inflammation and apoptosis were obviously observed, the mRNA expressions of TRAIL, TRAIL-sR1, CXCL10 and ICAM-1 were upregulated (P<0.05). Compared to high iodine group, thyroid absolute weight and relative weight in high iodine+poly (I:C) group were further increased, the level of total T_4 in serum was further decreased (P<0.05), the ratio of inflammatory degree Ⅳ increased to 50.0%, the numbers of apoptosis cells were further enhanced, the mRNA expressions of TRAIL, TRAIL-sR1, ICAM-1 and CXCL10 were further upregulated (P<0.05). Otherwise, the tendency of all parameters in poly (I:C) group was similar to that in control group (P>0.05). CONCLUSION: Poly (I:C) aggravates chronic lymphocytic thyroiditis induced by excess of iodine associated with increase in infiltration of lymphocytes and induction of apoptosis.