CAJ | 학술논문

利用酶联免疫法对猪肉中的磺胺类药物残留进行检测，结果表明：该方法的灵敏度为1μg，L，最低检测限为1．5μg/kg；向空白样本中添加浓度为100、200}xg／kg磺胺标准品时，平均回收率范围为76．1％。101．7％变异系数为4．6％-11．9％；利用该方法与液相色谱一串联质谱法检测实际样本，其阴、阳性判断结果一致。该法灵敏准确、重复性好、特异性高，适用于对猪肉中15种磺胺类药物进行多残留检测。
이용매련면역법대저육중적광알류약물잔류진행검측，결과표명：해방법적령민도위1μg，L，최저검측한위1．5μg/kg；향공백양본중첨가농도위100、200}xg／kg광알표준품시，평균회수솔범위위76．1％。101．7％변이계수위4．6％-11．9％；이용해방법여액상색보일천련질보법검측실제양본，기음、양성판단결과일치。해법령민준학、중복성호、특이성고，괄용우대저육중15충광알류약물진행다잔류검측。
The method of enzyme-linked immunosorbent assay （ ELISA ） was used to detect the residues of sulfonamides in pork. The results showed that the sensitivity and detection limit of ELISA were 1 μg/L and 1.5μg/kg, the range of average recovery was 76.1%-101.7%, and the coefficient of variation was 4.6% -11.9% when the blank samples were spiked with 100, 200 Ixg/kg. The judgement of positive and negative were the same when using ELISA and LC-MS/MS to detect the samples. The ELISA method was sensitive with high repeatability and specificity, and suitable for detecting sulfonamides residues in pork.