超声疗法%电刺激疗法%痛阈%脑啡肽,甲硫氨酸%脑啡肽,亮氨酸
超聲療法%電刺激療法%痛閾%腦啡肽,甲硫氨痠%腦啡肽,亮氨痠
초성요법%전자격요법%통역%뇌배태,갑류안산%뇌배태,량안산
背景:超声-中频电疗法具有良好的镇痛效果,该镇痛过程是否有脑啡肽等神经化学类物质参与,需对经超声-中频电作用后大鼠的疼痛反应进行定性、定量分析后验证.目的:观察超声-中频电治疗的镇痛作用及其对大鼠脑啡肽水平的影响,探讨其镇痛作用与脑啡肽神经化学物质变化间的相关性.设计:随机分组设计、对照动物实验.单位:中国医科大学附属一院理疗康复科,中国协和医科大学基础医学研究所.材料:实验于2002-06/2003-03在中国协和医科大学基础医学研究所完成.选择健康雌性Wistar大白鼠40只,随机分为4组,甲硫氨酸脑啡肽测定实验组,甲硫氨酸脑啡肽测定对照组,亮氨酸脑啡肽测定实验组,亮氨酸脑啡肽测定对照组,每组10只,其中甲硫氨酸脑啡肽组及亮氨酸脑啡肽组统称为实验组,甲硫氨酸脑啡肽对照组及亮氨酸脑啡肽对照组统称为对照组.方法:①对实验组大鼠进行超声-中频电治疗,频率0.8 MHz,采用连脉方式,调制频率100 Hz,超声波声强0.9 W/cm2;中频电载波频率4 kHz,调制频率100Hz,采用连调波型,中频电流为2 mA,作用时间为10 min.对照组也经同样处理,但超声-中频电治疗仪不开高压,故无能量输出.采用辐射热甩尾法测定大鼠痛觉,用秒表测定大鼠甩尾反应时间(s)并作为痛阈值.②当超声-中频电作用大鼠10 min后立即测痛阈,然后断头取其腺垂体(垂体后叶弃去)及丘脑下部组织,按脑啡肽放射免疫测定法测定其甲硫氨酸脑啡肽及亮氨酸脑啡肽含量.主要观察指标:治疗前后痛阈变化,甲硫氨酸脑啡肽和亮氨酸脑啡肽的含量.结果:纳入动物40只,均进入结果分析.①甲硫氨酸脑啡肽测定实验组和亮氨酸脑啡肽测定实验组大鼠痛阈值变化率分别为265.79%和272.90%,两组间比较差异无显著性(P＞0.05).②大鼠经超声-中频电单次作用后,其腺垂体及丘脑下部甲硫氨酸脑啡肽和亮氨酸脑啡肽含量均较治疗前显著升高[腺垂体甲硫氨酸脑啡肽和亮氨酸脑啡肽变化率分别为(300.48±36.21)%,(204.61±68.65)%,丘脑下部甲硫氨酸脑啡肽和亮氨酸脑啡肽变化率分别为(239.80±59.98)%,(205.53±51.62)%,P＜0.05].③经直线回归分析发现大鼠痛阈的变化值与腺垂体甲硫氨酸脑啡肽含量呈正相关(r=0.91,P＜0.01),即腺垂体甲硫氨酸脑啡肽的升高值与痛阚的升高值密切相关,当大鼠痛阈值升高100%时,腺垂体甲硫氨酸脑啡肽含量可较对照组升高117.02%.结论:腺垂体甲硫氨酸脑啡肽水平升高可能是超声-中频电疗法发挥镇痛作用的重要机制之一.
揹景:超聲-中頻電療法具有良好的鎮痛效果,該鎮痛過程是否有腦啡肽等神經化學類物質參與,需對經超聲-中頻電作用後大鼠的疼痛反應進行定性、定量分析後驗證.目的:觀察超聲-中頻電治療的鎮痛作用及其對大鼠腦啡肽水平的影響,探討其鎮痛作用與腦啡肽神經化學物質變化間的相關性.設計:隨機分組設計、對照動物實驗.單位:中國醫科大學附屬一院理療康複科,中國協和醫科大學基礎醫學研究所.材料:實驗于2002-06/2003-03在中國協和醫科大學基礎醫學研究所完成.選擇健康雌性Wistar大白鼠40隻,隨機分為4組,甲硫氨痠腦啡肽測定實驗組,甲硫氨痠腦啡肽測定對照組,亮氨痠腦啡肽測定實驗組,亮氨痠腦啡肽測定對照組,每組10隻,其中甲硫氨痠腦啡肽組及亮氨痠腦啡肽組統稱為實驗組,甲硫氨痠腦啡肽對照組及亮氨痠腦啡肽對照組統稱為對照組.方法:①對實驗組大鼠進行超聲-中頻電治療,頻率0.8 MHz,採用連脈方式,調製頻率100 Hz,超聲波聲彊0.9 W/cm2;中頻電載波頻率4 kHz,調製頻率100Hz,採用連調波型,中頻電流為2 mA,作用時間為10 min.對照組也經同樣處理,但超聲-中頻電治療儀不開高壓,故無能量輸齣.採用輻射熱甩尾法測定大鼠痛覺,用秒錶測定大鼠甩尾反應時間(s)併作為痛閾值.②噹超聲-中頻電作用大鼠10 min後立即測痛閾,然後斷頭取其腺垂體(垂體後葉棄去)及丘腦下部組織,按腦啡肽放射免疫測定法測定其甲硫氨痠腦啡肽及亮氨痠腦啡肽含量.主要觀察指標:治療前後痛閾變化,甲硫氨痠腦啡肽和亮氨痠腦啡肽的含量.結果:納入動物40隻,均進入結果分析.①甲硫氨痠腦啡肽測定實驗組和亮氨痠腦啡肽測定實驗組大鼠痛閾值變化率分彆為265.79%和272.90%,兩組間比較差異無顯著性(P＞0.05).②大鼠經超聲-中頻電單次作用後,其腺垂體及丘腦下部甲硫氨痠腦啡肽和亮氨痠腦啡肽含量均較治療前顯著升高[腺垂體甲硫氨痠腦啡肽和亮氨痠腦啡肽變化率分彆為(300.48±36.21)%,(204.61±68.65)%,丘腦下部甲硫氨痠腦啡肽和亮氨痠腦啡肽變化率分彆為(239.80±59.98)%,(205.53±51.62)%,P＜0.05].③經直線迴歸分析髮現大鼠痛閾的變化值與腺垂體甲硫氨痠腦啡肽含量呈正相關(r=0.91,P＜0.01),即腺垂體甲硫氨痠腦啡肽的升高值與痛闞的升高值密切相關,噹大鼠痛閾值升高100%時,腺垂體甲硫氨痠腦啡肽含量可較對照組升高117.02%.結論:腺垂體甲硫氨痠腦啡肽水平升高可能是超聲-中頻電療法髮揮鎮痛作用的重要機製之一.
배경:초성-중빈전요법구유량호적진통효과,해진통과정시부유뇌배태등신경화학류물질삼여,수대경초성-중빈전작용후대서적동통반응진행정성、정량분석후험증.목적:관찰초성-중빈전치료적진통작용급기대대서뇌배태수평적영향,탐토기진통작용여뇌배태신경화학물질변화간적상관성.설계:수궤분조설계、대조동물실험.단위:중국의과대학부속일원리료강복과,중국협화의과대학기출의학연구소.재료:실험우2002-06/2003-03재중국협화의과대학기출의학연구소완성.선택건강자성Wistar대백서40지,수궤분위4조,갑류안산뇌배태측정실험조,갑류안산뇌배태측정대조조,량안산뇌배태측정실험조,량안산뇌배태측정대조조,매조10지,기중갑류안산뇌배태조급량안산뇌배태조통칭위실험조,갑류안산뇌배태대조조급량안산뇌배태대조조통칭위대조조.방법:①대실험조대서진행초성-중빈전치료,빈솔0.8 MHz,채용련맥방식,조제빈솔100 Hz,초성파성강0.9 W/cm2;중빈전재파빈솔4 kHz,조제빈솔100Hz,채용련조파형,중빈전류위2 mA,작용시간위10 min.대조조야경동양처리,단초성-중빈전치료의불개고압,고무능량수출.채용복사열솔미법측정대서통각,용초표측정대서솔미반응시간(s)병작위통역치.②당초성-중빈전작용대서10 min후립즉측통역,연후단두취기선수체(수체후협기거)급구뇌하부조직,안뇌배태방사면역측정법측정기갑류안산뇌배태급량안산뇌배태함량.주요관찰지표:치료전후통역변화,갑류안산뇌배태화량안산뇌배태적함량.결과:납입동물40지,균진입결과분석.①갑류안산뇌배태측정실험조화량안산뇌배태측정실험조대서통역치변화솔분별위265.79%화272.90%,량조간비교차이무현저성(P＞0.05).②대서경초성-중빈전단차작용후,기선수체급구뇌하부갑류안산뇌배태화량안산뇌배태함량균교치료전현저승고[선수체갑류안산뇌배태화량안산뇌배태변화솔분별위(300.48±36.21)%,(204.61±68.65)%,구뇌하부갑류안산뇌배태화량안산뇌배태변화솔분별위(239.80±59.98)%,(205.53±51.62)%,P＜0.05].③경직선회귀분석발현대서통역적변화치여선수체갑류안산뇌배태함량정정상관(r=0.91,P＜0.01),즉선수체갑류안산뇌배태적승고치여통감적승고치밀절상관,당대서통역치승고100%시,선수체갑류안산뇌배태함량가교대조조승고117.02%.결론:선수체갑류안산뇌배태수평승고가능시초성-중빈전요법발휘진통작용적중요궤제지일.
BACKGROUND: Ultrasound-medium frequency electrotherapy has better pain-relieving effect,whether it involves enkephalin and the like neurotransmitter is still not very clear, it is necessary to carry out qualitative and quantitative analysis of the pain response in rats received ultrasound-medium frequency electrotherapy.OBJECTIVE: To investigate the pain-relieving effect of ultrasound-medium frequency electrotherapy and its influence on the enkephalin content in rats, aiming to probe its underlying mechanism and relationship with neurotransmitter enkephalin.DESIGN: Randomly controlled study taking experimental animals as subjects.SETTING: Department of Physiotherapy, First Hospital Affiliated to China Medical University, Institute of Basic Medicine, Peking Union Medical CollegeMATERIALS: This experiment was carried out at the Institute of Basic Medicine, Peking Union Medical College between June 2002 and March 2003. Forty healthy male Wistar rats were adopted and randomized into four groups,namely methionine enkephalin detection experimental group and control group,as well as leucine enkephalin detection experimental group and control group with 10 rats in each group ,amongst which methionine enkephalin experimental group and leucine enkephalin experimental group were generally designed as experimental group ,with the other two group as control group.METHODS: ①Rats ín the experímental group were subjected to ultrasound-medium frequency electrotherapy with frequency of 0.8 MHz in manner of geminal pulse,the modulatìng frequency was 100 Hz and ultrasound intensity of 0.9 W/cm2;the carrier frequency of medium frequency electricity was 4 kHz with modulating frequency of 100 Hz in manner of continuous wave,the electric current of medium frequency was 2 mA and acting time of 10 min. rats in control group received the same dealing,but the higher voltage of ultrasound-medium frequency electrotheprauetic instrument was not used, thereby no energy output was available. tail flick test(TFr) was used to test rat algesthesia,and stopwatch was used to record the time for tail flick(s) that was taken as the value of pain threshold. ②The threshold of pain was tested at 10 minutes instantly after ultrasoundmedium frequency electric stimulation,and then pituitary (removing the posterior pituitary lobe) and hypothalamus were obtained to detect the content of methionine enkephalin and leucine enkephalin by using radioimmuno assay.MAIN OUTCOME MEASURES: The changes of the pain threshold after treatment,the content of methionine enkephalin and leucine enkephalin.RESULTS: Totally 40 rats were enrolled in the experiment and entered in the results analysis. ① The changing rate of pain threshold in two experimental group were 265.79% and 272.90% respectively,the difference was of no statistical significance (P ＞ 0.05). ②After ultrasoundmedium frequency electrotherapy,the content of methionine enkephalin and leucine enkephalin in pituitary and hypothalamus were obviously higher than before treatment [ The changing rate of methionine enkephalin and leucine enkephalin in adenohypophysis was 300.48 ±36.21)% and(204.61±68.65)% , respectively, compared to (239.80±59.98)%and(205.53±51.62)% in hypothalamus, P ＜ 0.05]. ③Linear regression analysis revealed that the variance of the pain threshold was positively correlated with the content of methionine enkephalin in adenohypophysis(r=0.91 ,P ＜ 0.01), suggesting the increment of methionine enkephalin in adenohypophysis was closely connected with the increment of pain threshold,and the level of methionine enkephalin in adenohypophysis would be higher than that in control group by 117.02% when the value of pain threshold increased by100%.CONCLUSION: One of the important mechanisms for the pain-relieving effect of ultrasound-medium frequency electrotherapy might be the increase of methionine enkephalin in adenohypophysis.
