中国实用眼科杂志
中國實用眼科雜誌
중국실용안과잡지
CHINESE JOURNAL OF PRACTICAL OPHTHALMOLOGY
2009年
7期
771-775
,共5页
姜洋%李莹%陈秉钧%龙琴
薑洋%李瑩%陳秉鈞%龍琴
강양%리형%진병균%룡금
实验性近视%离焦%巩膜%基质金属蛋白酶-2抑制剂
實驗性近視%離焦%鞏膜%基質金屬蛋白酶-2抑製劑
실험성근시%리초%공막%기질금속단백매-2억제제
Experimental myopia%Defocus%Sclera%Matrix metalloproteinase 2 inhibitor
目的 探讨基质金属蛋白酶-2(MMP-2)特异性抑制剂1-(12-羟)18烷基硫酸钠对负透镜诱导豚鼠离焦性近视形成的影响及其作用机制.方法 对新生健康有色豚鼠随机分为3组,A组(10只):右眼进行-10D透镜诱导,左眼不作处理;B组(10只):右眼透镜诱导+抑制剂(9.0μg/ml,每3d结膜下注射),左眼不作处理;C组(10只):右眼透镜诱导+赋形剂,左眼不作处理,4周后测量屈光度,明胶酶谱法分析后部巩膜MMP-2活性,western-blot 技术检测后部巩膜MMP-2的表达.结果豚鼠经4周负透镜诱导后,A组诱导眼较对侧眼增加了(5.73±0.36)D近视度;B组诱导眼较对侧眼增加了(2.58±0.33)D近视度;C组诱导眼较对侧眼增加了(6.31±0.73)D近视度;B组所获得的近视度低于A组和C组,差异有统计学意义(q检验,P<0.05),A组和C组相比差异无统计学意义(P>0.05).B组抑制剂注射眼后部巩膜MMP-2活性成分低于A组和C组,差异有统计学意义(P<0.05),酶原成分3组间比较差异无统计学意义(P>0.05).3组后部巩膜MMP-2蛋白水平差异无统计学意义(P>0.05).结论 9.0μg/ml 1-(12-羟)18烷基硫酸钠,每3d结膜下注射可通过抑制后部巩膜MMP-2的活性,从而在一定程度上抑制新生豚鼠负透镜诱导离焦性近视的发展.
目的 探討基質金屬蛋白酶-2(MMP-2)特異性抑製劑1-(12-羥)18烷基硫痠鈉對負透鏡誘導豚鼠離焦性近視形成的影響及其作用機製.方法 對新生健康有色豚鼠隨機分為3組,A組(10隻):右眼進行-10D透鏡誘導,左眼不作處理;B組(10隻):右眼透鏡誘導+抑製劑(9.0μg/ml,每3d結膜下註射),左眼不作處理;C組(10隻):右眼透鏡誘導+賦形劑,左眼不作處理,4週後測量屈光度,明膠酶譜法分析後部鞏膜MMP-2活性,western-blot 技術檢測後部鞏膜MMP-2的錶達.結果豚鼠經4週負透鏡誘導後,A組誘導眼較對側眼增加瞭(5.73±0.36)D近視度;B組誘導眼較對側眼增加瞭(2.58±0.33)D近視度;C組誘導眼較對側眼增加瞭(6.31±0.73)D近視度;B組所穫得的近視度低于A組和C組,差異有統計學意義(q檢驗,P<0.05),A組和C組相比差異無統計學意義(P>0.05).B組抑製劑註射眼後部鞏膜MMP-2活性成分低于A組和C組,差異有統計學意義(P<0.05),酶原成分3組間比較差異無統計學意義(P>0.05).3組後部鞏膜MMP-2蛋白水平差異無統計學意義(P>0.05).結論 9.0μg/ml 1-(12-羥)18烷基硫痠鈉,每3d結膜下註射可通過抑製後部鞏膜MMP-2的活性,從而在一定程度上抑製新生豚鼠負透鏡誘導離焦性近視的髮展.
목적 탐토기질금속단백매-2(MMP-2)특이성억제제1-(12-간)18완기류산납대부투경유도돈서리초성근시형성적영향급기작용궤제.방법 대신생건강유색돈서수궤분위3조,A조(10지):우안진행-10D투경유도,좌안불작처리;B조(10지):우안투경유도+억제제(9.0μg/ml,매3d결막하주사),좌안불작처리;C조(10지):우안투경유도+부형제,좌안불작처리,4주후측량굴광도,명효매보법분석후부공막MMP-2활성,western-blot 기술검측후부공막MMP-2적표체.결과돈서경4주부투경유도후,A조유도안교대측안증가료(5.73±0.36)D근시도;B조유도안교대측안증가료(2.58±0.33)D근시도;C조유도안교대측안증가료(6.31±0.73)D근시도;B조소획득적근시도저우A조화C조,차이유통계학의의(q검험,P<0.05),A조화C조상비차이무통계학의의(P>0.05).B조억제제주사안후부공막MMP-2활성성분저우A조화C조,차이유통계학의의(P<0.05),매원성분3조간비교차이무통계학의의(P>0.05).3조후부공막MMP-2단백수평차이무통계학의의(P>0.05).결론 9.0μg/ml 1-(12-간)18완기류산납,매3d결막하주사가통과억제후부공막MMP-2적활성,종이재일정정도상억제신생돈서부투경유도리초성근시적발전.
Objective To investigate the inhibitory effects of Sodium 1-(12-Hydroxy)octadecanyl Sulfate,an MMP-2 inhibitor,on the development of defocus-induced myopia in guinea pigs and its mechanism.Methods Thirty newborn guinea pigs were randomly divided into 3 groups,group A: -10D lens applied on right eye;group B: -10D lens and MMP-2 inhibitor treatment in right eye(9.0μg/ml,subconjunctival injection,every 3 days interval);group C: -10D lens and vehicle treatment in right eye(same volume and frequency with those of group B);there was no treatment in all the left eyes in this study.After 28 days of defocus,the refractive status was recorded and the posterior scleral sample was collected for MMP-2 zymography and western-blot analysis.Results The treated eyes of group A,B and C gained(5.73±0.36)D,(2.58±0.33)D and(6.31±0.73)D respectively,the amount of gained myopic refraction in eyes of group A was significantly less than that of eyes in group B and group C(P<0.05);zymography analysis indicated that,of all the defocused animals,the activity of MMP-2 in the eyes treated by inhibitor was significantly lower than that of eyes treated by vehicle and no injected eyes,represented by the lower level of active MMP-2(P<0.05);no significant difference was detected in the latent MMP-2 level between three groups(P>0.05);the MMP-2 protein level in posterior sclera of three groups also didn't show any significant difference by western-blot analysis(p>0.05).Conclusions 1-(12-Hydroxy)octadecanyl Sulfate(9.0μg/ml,subconjunctival injection,every 3 days interval)inhibited the development of defocus-induced myopia in newborn guinea pigs,may through,at least in part,inhibitory effects on the activity of MMP-2 in posterior sclera.
