中华结核和呼吸杂志
中華結覈和呼吸雜誌
중화결핵화호흡잡지
Chinese Journal of Tuberculosis and Respiratory Diseases
2010年
1期
43-45
,共3页
桂晓虹%徐鹏%赵明%王莉莉%武洁%李静%高谦%梅建
桂曉虹%徐鵬%趙明%王莉莉%武潔%李靜%高謙%梅建
계효홍%서붕%조명%왕리리%무길%리정%고겸%매건
试剂盒%诊断%分枝杆菌%结核%利福平%异烟肼
試劑盒%診斷%分枝桿菌%結覈%利福平%異煙肼
시제합%진단%분지간균%결핵%리복평%이연정
Reagent kits,diagnostic%Mycobacterium tuberculosis%Rifampin%Isoniazid
目的 评价用耐多药结核病快速诊断(GenoType MTBDRplus)试剂盒检测耐利福平、异炳肼MTB的效果.方法 选取75株MTB临床分离株,采用耐多药结核病快速诊断试剂盒检测其中的耐药MTB,评价其敏感度和特异度及可重复性,并与常规的药物敏感性试验方法相比较.结果 耐多药结核病快速诊断试剂盒对上海地区的MTB临床分离株中耐利福平和耐异烟肼菌株的敏感度分别为98.0%和86.5%,对敏感菌株的特异度为100%.而在重复性试验中,耐多药结核病快速诊断试剂盒多次结果完全一致,重复性达到100%.结论 耐多药结核病快速诊断作为快速检测耐药MTB的试剂盒,对耐利福平和耐异烟肼菌株具有较高的敏感度和特异度,在上海地区有较好的应用前景.
目的 評價用耐多藥結覈病快速診斷(GenoType MTBDRplus)試劑盒檢測耐利福平、異炳肼MTB的效果.方法 選取75株MTB臨床分離株,採用耐多藥結覈病快速診斷試劑盒檢測其中的耐藥MTB,評價其敏感度和特異度及可重複性,併與常規的藥物敏感性試驗方法相比較.結果 耐多藥結覈病快速診斷試劑盒對上海地區的MTB臨床分離株中耐利福平和耐異煙肼菌株的敏感度分彆為98.0%和86.5%,對敏感菌株的特異度為100%.而在重複性試驗中,耐多藥結覈病快速診斷試劑盒多次結果完全一緻,重複性達到100%.結論 耐多藥結覈病快速診斷作為快速檢測耐藥MTB的試劑盒,對耐利福平和耐異煙肼菌株具有較高的敏感度和特異度,在上海地區有較好的應用前景.
목적 평개용내다약결핵병쾌속진단(GenoType MTBDRplus)시제합검측내리복평、이병정MTB적효과.방법 선취75주MTB림상분리주,채용내다약결핵병쾌속진단시제합검측기중적내약MTB,평개기민감도화특이도급가중복성,병여상규적약물민감성시험방법상비교.결과 내다약결핵병쾌속진단시제합대상해지구적MTB림상분리주중내리복평화내이연정균주적민감도분별위98.0%화86.5%,대민감균주적특이도위100%.이재중복성시험중,내다약결핵병쾌속진단시제합다차결과완전일치,중복성체도100%.결론 내다약결핵병쾌속진단작위쾌속검측내약MTB적시제합,대내리복평화내이연정균주구유교고적민감도화특이도,재상해지구유교호적응용전경.
Objective To evaluate the ability of GenoType MTBDRplas Assay for detection of rifampin(RFP)and isoniazid(INH)resitance in Mycobacterium tuberculosis.Methods Seventy-five clinical isolates were tested for tranditional drug susceptibility testing and GenoType MTBDRplus assay.The results were compared to assess the sensitivity and specificity of GenoType(R) MTBDRplus assay.Results The sensitivity of GenoType MTBDRplus assays for detection of RFP and INH resistance were 98.0% and 86.5%,respectively.In pan-susceptible isolates of Mycobacterium tuberculosis,the specificity was 100% for both RFP resistance and INH resistance.Conclusion With its high sensitivity and specificity,GenoType MTBDRplus assay is a promising rapid method to detect INH and RFP resistant Mycobacterium tuberculosis strains in Shanghai.
