中华神经医学杂志
中華神經醫學雜誌
중화신경의학잡지
CHINESE JOURNAL OF NEUROMEDICINE
2010年
5期
465-468
,共4页
何俊德%谢泽宇%陈葆%瞿文军
何俊德%謝澤宇%陳葆%瞿文軍
하준덕%사택우%진보%구문군
颅脑损伤%高压氧%水迷宫试验
顱腦損傷%高壓氧%水迷宮試驗
로뇌손상%고압양%수미궁시험
Cerebral trauma%Hyperbaric oxygen%Morris water maze method
目的 研究不同时间窗高压氧治疗对脑外伤大鼠认知功能障碍的治疗效果. 方法 将96只大鼠按随机数字表法分为正常对照组(A组)、液压冲击脑外伤模型组(B组)、液压冲击脑外伤模型+常规治疗组(C组)、液压冲击脑外伤模型+常规治疗+高压氧治疗组(D组),每组24只;D组在模型建立后根据高压氧治疗的时间点(3、12、24、72 h)分为4亚组(D-3 h组、D-12 h组、D-24 h组和D-72h组,每亚组6只).B、C、D组采用大鼠侧位液压冲击构建脑外伤模型后分别进行不同处理.应用水迷宫试验对比评价各组大鼠模型脑外伤后认知功能障碍的改变. 结果 在相同时间点寻找水下平台的潜伏期方面,B组最长,其次为C组、D组、A组,且D组中D.72 h组最长,其次为D-24 h组、D-12h组、D-3h组;在穿过原平台区域的次数方面,A组最多,其次为D组、C组、B组,且D组中D-3 h组最多,其次为D-12 h组、D-24 h组、D-72 h组,与B组比较差异均有统计学意义(p<0.05). 结论 高压氧治疗可以改善颅脑损伤大鼠的学习能力,对神经功能具有保护作用,且这种高压氧治疗的时间最好在损伤后12 h内完成,并且宜尽早实施.
目的 研究不同時間窗高壓氧治療對腦外傷大鼠認知功能障礙的治療效果. 方法 將96隻大鼠按隨機數字錶法分為正常對照組(A組)、液壓遲擊腦外傷模型組(B組)、液壓遲擊腦外傷模型+常規治療組(C組)、液壓遲擊腦外傷模型+常規治療+高壓氧治療組(D組),每組24隻;D組在模型建立後根據高壓氧治療的時間點(3、12、24、72 h)分為4亞組(D-3 h組、D-12 h組、D-24 h組和D-72h組,每亞組6隻).B、C、D組採用大鼠側位液壓遲擊構建腦外傷模型後分彆進行不同處理.應用水迷宮試驗對比評價各組大鼠模型腦外傷後認知功能障礙的改變. 結果 在相同時間點尋找水下平檯的潛伏期方麵,B組最長,其次為C組、D組、A組,且D組中D.72 h組最長,其次為D-24 h組、D-12h組、D-3h組;在穿過原平檯區域的次數方麵,A組最多,其次為D組、C組、B組,且D組中D-3 h組最多,其次為D-12 h組、D-24 h組、D-72 h組,與B組比較差異均有統計學意義(p<0.05). 結論 高壓氧治療可以改善顱腦損傷大鼠的學習能力,對神經功能具有保護作用,且這種高壓氧治療的時間最好在損傷後12 h內完成,併且宜儘早實施.
목적 연구불동시간창고압양치료대뇌외상대서인지공능장애적치료효과. 방법 장96지대서안수궤수자표법분위정상대조조(A조)、액압충격뇌외상모형조(B조)、액압충격뇌외상모형+상규치료조(C조)、액압충격뇌외상모형+상규치료+고압양치료조(D조),매조24지;D조재모형건립후근거고압양치료적시간점(3、12、24、72 h)분위4아조(D-3 h조、D-12 h조、D-24 h조화D-72h조,매아조6지).B、C、D조채용대서측위액압충격구건뇌외상모형후분별진행불동처리.응용수미궁시험대비평개각조대서모형뇌외상후인지공능장애적개변. 결과 재상동시간점심조수하평태적잠복기방면,B조최장,기차위C조、D조、A조,차D조중D.72 h조최장,기차위D-24 h조、D-12h조、D-3h조;재천과원평태구역적차수방면,A조최다,기차위D조、C조、B조,차D조중D-3 h조최다,기차위D-12 h조、D-24 h조、D-72 h조,여B조비교차이균유통계학의의(p<0.05). 결론 고압양치료가이개선로뇌손상대서적학습능력,대신경공능구유보호작용,차저충고압양치료적시간최호재손상후12 h내완성,병차의진조실시.
Objective To investigate the effects of hyperbaric oxygen (HBO) therapy performed at different times on the cognitive dysfunction of rats with traumatic brain injury. Methods The traumatic brain injured models were established by use of lateral fluid percussion in rats. Ninety-six rats were equally randomized into 4 groups: control group (group A), traumatic brain injured model group (group B), traumatic brain injured model plus conventional therapy group (group C), traumatic brain injured model plus both conventional therapy and HBO therapy group (group D). And group D also divided into 4 subgroups (n=6): D-3 h group, D-12 h group, D-24 h group and D-72 h groups that HBO therapy was performed at 3, 12, 24 and 72 h of the traumatic brain injury, respectively. The changes of learning and memory abilities before or after the therapy in each group were tested by Morris water maze method. Results Group B had the longest latency of finding the underwater platform as compared with the other 3 groups, followed by group C, group D and group A; as to the 4 sub-groups, D-72 h group had the longest latency of finding the underwater platform as compared with the other 3 groups, followed by group D-24 h, group D-12 h and group D-3 h. Group A could cross the original platform in the spatial probe test most often among all the 4 groups, followed by group D, group C and group B; group A, group C and group D was significantly different in clossing the original platform in the spatial probe test as compared with group B (P<0.05); group D-3 h could cross the original platform in the spatial probe test most often among all the 4 sub-groups, followed by group D-12 h, group D-24 h and group D-72 h.Conclusion HBO treatment can effectively improve the cognitive dysfunction of rats with traumatic brain injury, especially those within 12 h of injury.
