中华预防医学杂志
中華預防醫學雜誌
중화예방의학잡지
CHINESE JOURNAL OF
2010年
1期
30-33
,共4页
陈刚%黄春妍%仲齐庆%鞠晶昀%潘少聪%王春%姚陈娟
陳剛%黃春妍%仲齊慶%鞠晶昀%潘少聰%王春%姚陳娟
진강%황춘연%중제경%국정윤%반소총%왕춘%요진연
酚类%色谱法%液相%血药浓度%时间因素
酚類%色譜法%液相%血藥濃度%時間因素
분류%색보법%액상%혈약농도%시간인소
Phenols%Chromatography%liquid%Plasma concentration%Time factors
目的 探讨经口给予雄性大鼠双酚A(bisphenol A,BPA)后,血清中BPA时间-浓度关系.方法 66只无特定病原体(specific pathogen free,SPF)级雄性SD大鼠应用数字表法随机分为10个实验组和1个对照组,每组6只.各实验组大鼠一次性灌胃给予300 mg/kg剂量的BPA后,在0.5、1、2、4、6、12、24、36、60、84 h时间点各采集一组大鼠血液,分离出血清,用荧光-高效液相色谱法检测血清中游离型和总BPA浓度.结果 大鼠一次性经口染毒后,1 h血清中总BPA浓度最高,达17.13μg/ml,2 h血清中BPA浓度急剧下降,24 h血清中总BPA浓度又上升到15.18μg/ml,持续观察84 h,血清中BPA仍有0.51μg/ml.游离型BPA含量较低,染毒0.5 h,血清中游离型BPA浓度为0.57 μg/ml,而后急剧下降,1 h下降到0.06μg/ml,4 h下降到0.03μg/ml,36 h仅有0.01μg/ml.在染毒0.5 h,游离型BPA在血清中所占的百分率最高,为血清总BPA的4.15%(0.57/13.73),其他各时间点血清中游离型BPA所占的百分率均较低.结论一次性经口给予大鼠BPA后,血清中主要以结合型BPA存在,血清总BPA随染毒时间的延长出现双峰现象,可能与BPA代谢过程中肝肠循环有关.
目的 探討經口給予雄性大鼠雙酚A(bisphenol A,BPA)後,血清中BPA時間-濃度關繫.方法 66隻無特定病原體(specific pathogen free,SPF)級雄性SD大鼠應用數字錶法隨機分為10箇實驗組和1箇對照組,每組6隻.各實驗組大鼠一次性灌胃給予300 mg/kg劑量的BPA後,在0.5、1、2、4、6、12、24、36、60、84 h時間點各採集一組大鼠血液,分離齣血清,用熒光-高效液相色譜法檢測血清中遊離型和總BPA濃度.結果 大鼠一次性經口染毒後,1 h血清中總BPA濃度最高,達17.13μg/ml,2 h血清中BPA濃度急劇下降,24 h血清中總BPA濃度又上升到15.18μg/ml,持續觀察84 h,血清中BPA仍有0.51μg/ml.遊離型BPA含量較低,染毒0.5 h,血清中遊離型BPA濃度為0.57 μg/ml,而後急劇下降,1 h下降到0.06μg/ml,4 h下降到0.03μg/ml,36 h僅有0.01μg/ml.在染毒0.5 h,遊離型BPA在血清中所佔的百分率最高,為血清總BPA的4.15%(0.57/13.73),其他各時間點血清中遊離型BPA所佔的百分率均較低.結論一次性經口給予大鼠BPA後,血清中主要以結閤型BPA存在,血清總BPA隨染毒時間的延長齣現雙峰現象,可能與BPA代謝過程中肝腸循環有關.
목적 탐토경구급여웅성대서쌍분A(bisphenol A,BPA)후,혈청중BPA시간-농도관계.방법 66지무특정병원체(specific pathogen free,SPF)급웅성SD대서응용수자표법수궤분위10개실험조화1개대조조,매조6지.각실험조대서일차성관위급여300 mg/kg제량적BPA후,재0.5、1、2、4、6、12、24、36、60、84 h시간점각채집일조대서혈액,분리출혈청,용형광-고효액상색보법검측혈청중유리형화총BPA농도.결과 대서일차성경구염독후,1 h혈청중총BPA농도최고,체17.13μg/ml,2 h혈청중BPA농도급극하강,24 h혈청중총BPA농도우상승도15.18μg/ml,지속관찰84 h,혈청중BPA잉유0.51μg/ml.유리형BPA함량교저,염독0.5 h,혈청중유리형BPA농도위0.57 μg/ml,이후급극하강,1 h하강도0.06μg/ml,4 h하강도0.03μg/ml,36 h부유0.01μg/ml.재염독0.5 h,유리형BPA재혈청중소점적백분솔최고,위혈청총BPA적4.15%(0.57/13.73),기타각시간점혈청중유리형BPA소점적백분솔균교저.결론일차성경구급여대서BPA후,혈청중주요이결합형BPA존재,혈청총BPA수염독시간적연장출현쌍봉현상,가능여BPA대사과정중간장순배유관.
Objective To investigate the relationship of time-concentration of bisphenol A (BPA) in male Sprague-Dawley (SD) rats after single oral BPA administration. Methods A total of 66 specific pathogen free (SPF) SD male rats were divided into 10 experimental groups and control group (n =6). The experimental group rats were treated with BPA of 300 mg/kg by oral garage and blood samples were taken from one group at 0. 5,1,2,4,6, 12,24,36,60,84 h time point after oral administration, respectively. The serum BPA concentration was determined by fluorescence-high performance liquid chromatography(FL-HPLC) analysis. Results After oral administration of 300 mg/kg, the total serum BPA concentration of 17. 13 μg/ml was the highest in rats at 1 h,then decreased,but it increased to 15.18 μ/ml again at 24 h,then gradually decreased to 0. 51 μg/ml at 84 h. The level of serum free BPA was lower than that of total serum BPA after oral administration, the serum free BPA was 0. 57μg/ml at 0. 5 h after oral administration. The serum free BPA level decreased to 0. 06 μg/ml at 1 h,0. 03 μg/ml at4 h,0.01 μg/ml at 36 h after oral administration. The free BPA was only 4. 15% (0. 57/13.73) in total BPA in serum at 0. 5 h after oral administration of 300 mg/kg BPA. Conclusion These results suggested that conjugated BPA was the main metabolite of BPA in rat serum after single oral administration. Enterohepatic circulation of BPA glucuronide in rats may results in two peak levels of total BPA in serum.
