上海第二医科大学学报(英文版)
上海第二醫科大學學報(英文版)
상해제이의과대학학보(영문판)
JOURNAL OF SHANGHAI SECOND MEDICAL UNIVERSITY
2003年
1期
21-24
,共4页
刘伟%蔡泽浩%商庆新%钱云良%曹谊林
劉偉%蔡澤浩%商慶新%錢雲良%曹誼林
류위%채택호%상경신%전운량%조의림
TGF-β1%autoproduction%signal transduction%gene therapy
Objective To determine over expression of a truncated type Ⅱ TGF-β receptor in down regulating TGF-β1 autoproduction in normal dermal fibroblasts.MethodsIn vitro cultured dermal fibroblasts were treated with rhTGF β1(5ng/ml) or recombinant adenovirus containing a truncated type Ⅱ TGF-β receptor gene(50pfu/cell). Their effects on regulating gene expression of TGF β1 were observed with Northern Blot.Resultsrh TGF β1 up regulated the gene expression of TGF-β1(34%～150%) and type Ⅰ procollagen(13%～190%).Over expression of a truncated receptor Ⅱ decreased the gene expression of TGF-β1(53%～66%).ConclusionOver expression of the truncated TGF-β receptor Ⅱ down regulated TGF-β1 autoproduction via blocking signal transduction of TGF-β. This study may provide a new strategy for scar gene therapy.
