心力衰竭,充血性%学习,记忆%大鼠,Sprague-Dawley
心力衰竭,充血性%學習,記憶%大鼠,Sprague-Dawley
심력쇠갈,충혈성%학습,기억%대서,Sprague-Dawley
背景:慢性充血性心力衰竭是许多心脏疾病的晚期表现,大多数临床研究报道支持充血性心力衰竭时存在认知障碍,而成功的心功能改善措施具有提高认知功能的作用.目的:探讨心肌梗死后充血性心力衰竭对大鼠空间学习和记忆能力的影响,观察心力衰竭与认知功能之间的相关性.设计:以实验动物为观察对象的随机对照观察.单位:北京中医药大学东直门医院教育部中医内科学重点实验室暨北京市中医内科学重点实验室.材料:实验于2001-11在北京中医药大学东直门医院教育部(北京市)重点实验室完成.选择购于北京维通利华实验动物技术有限公司的10～12周龄健康雄性SD大鼠100只,体质量(200～220)g,随机分为手术组(n=60)和假手术组(n=40).方法:手术组大鼠采用结扎左冠状动脉前降支的方法制作急性心肌梗死模型,假手术组大鼠在左冠状动脉前降支留置一松结,不结扎.将心电图示8个导联有心肌梗死样改变的存活大鼠作为符合条件的手术组大鼠(n=33),心电图没有异常改变的未结扎存活大鼠作为符合条件的假手术组大鼠(n=25),将两组分别按时间分为10 d组、30 d组、60 d组.分别于术后10、30、60 d进行Morris水迷宫实验,并测血流动力学指标.在手术10 d组、30 d组和60 d组中,由于心力衰竭(简称心衰)体力不济而无法完成全部Morris游泳实验,且在实验后2 d内死亡或血流动力学测定后死亡的大鼠组成心衰终末期组,其对照组为与死亡大鼠同组的体质量相近的手术组大鼠.主要观察指标:①逃避对数潜伏期和认知能力较好率.②每搏量、每分心排出量、心率和心指数值,其中心指数值为主要反应心功能的指标.结果:符合条件的手术组大鼠33只,假手术组大鼠25只,进入结果分析.①血流动力学指标测定结果:手术10 d、30 d、60 d组大鼠的每搏量、每分心排出量、心指数均明显低于同期假手术组(P＜0.01),心衰终末期组则显著低于终末对照组及手术组各期大鼠(P＜0.01-0.05),其中手术组与同期假手术组,心衰终末期组及终末对照组心指数比值分别为51.21%、50.58%、55.84%和33.91%,均低于60%.②Morris水迷宫测验结果:手术10 d组后8次训练对数潜伏期较假手术10 d组明显延长[F(1,28)=5.997,P=0.021],搜索策略方面手术10 d组的认知功能明显比假手术10 d组差(x2=8.142,P＜0.005).手术30 d组与假手术30 d组比较没有明显变化.手术60d组与假手术60 d组大鼠对数潜伏期没有显著差异,搜索策略方面,手术60d组总的认知能力较好率较假手术60 d组差(x2=4.988,P＜0.05).心衰终末期组与终末对照组对数潜伏期明显延长[F(1,6)=19.567,P=0.004],搜索策略方面终末期组总的认知能力较好率较终末对照组差(x2=11.82,P＜0.01).结论:心肌梗死充血性心衰后大鼠的空间学习记忆能力,在10 d时明显受损,在30 d时恢复正常,到60d时开始出现一定程度的认知损伤,在充血性心衰终末期时认知功能明显变差.
揹景:慢性充血性心力衰竭是許多心髒疾病的晚期錶現,大多數臨床研究報道支持充血性心力衰竭時存在認知障礙,而成功的心功能改善措施具有提高認知功能的作用.目的:探討心肌梗死後充血性心力衰竭對大鼠空間學習和記憶能力的影響,觀察心力衰竭與認知功能之間的相關性.設計:以實驗動物為觀察對象的隨機對照觀察.單位:北京中醫藥大學東直門醫院教育部中醫內科學重點實驗室暨北京市中醫內科學重點實驗室.材料:實驗于2001-11在北京中醫藥大學東直門醫院教育部(北京市)重點實驗室完成.選擇購于北京維通利華實驗動物技術有限公司的10～12週齡健康雄性SD大鼠100隻,體質量(200～220)g,隨機分為手術組(n=60)和假手術組(n=40).方法:手術組大鼠採用結扎左冠狀動脈前降支的方法製作急性心肌梗死模型,假手術組大鼠在左冠狀動脈前降支留置一鬆結,不結扎.將心電圖示8箇導聯有心肌梗死樣改變的存活大鼠作為符閤條件的手術組大鼠(n=33),心電圖沒有異常改變的未結扎存活大鼠作為符閤條件的假手術組大鼠(n=25),將兩組分彆按時間分為10 d組、30 d組、60 d組.分彆于術後10、30、60 d進行Morris水迷宮實驗,併測血流動力學指標.在手術10 d組、30 d組和60 d組中,由于心力衰竭(簡稱心衰)體力不濟而無法完成全部Morris遊泳實驗,且在實驗後2 d內死亡或血流動力學測定後死亡的大鼠組成心衰終末期組,其對照組為與死亡大鼠同組的體質量相近的手術組大鼠.主要觀察指標:①逃避對數潛伏期和認知能力較好率.②每搏量、每分心排齣量、心率和心指數值,其中心指數值為主要反應心功能的指標.結果:符閤條件的手術組大鼠33隻,假手術組大鼠25隻,進入結果分析.①血流動力學指標測定結果:手術10 d、30 d、60 d組大鼠的每搏量、每分心排齣量、心指數均明顯低于同期假手術組(P＜0.01),心衰終末期組則顯著低于終末對照組及手術組各期大鼠(P＜0.01-0.05),其中手術組與同期假手術組,心衰終末期組及終末對照組心指數比值分彆為51.21%、50.58%、55.84%和33.91%,均低于60%.②Morris水迷宮測驗結果:手術10 d組後8次訓練對數潛伏期較假手術10 d組明顯延長[F(1,28)=5.997,P=0.021],搜索策略方麵手術10 d組的認知功能明顯比假手術10 d組差(x2=8.142,P＜0.005).手術30 d組與假手術30 d組比較沒有明顯變化.手術60d組與假手術60 d組大鼠對數潛伏期沒有顯著差異,搜索策略方麵,手術60d組總的認知能力較好率較假手術60 d組差(x2=4.988,P＜0.05).心衰終末期組與終末對照組對數潛伏期明顯延長[F(1,6)=19.567,P=0.004],搜索策略方麵終末期組總的認知能力較好率較終末對照組差(x2=11.82,P＜0.01).結論:心肌梗死充血性心衰後大鼠的空間學習記憶能力,在10 d時明顯受損,在30 d時恢複正常,到60d時開始齣現一定程度的認知損傷,在充血性心衰終末期時認知功能明顯變差.
배경:만성충혈성심력쇠갈시허다심장질병적만기표현,대다수림상연구보도지지충혈성심력쇠갈시존재인지장애,이성공적심공능개선조시구유제고인지공능적작용.목적:탐토심기경사후충혈성심력쇠갈대대서공간학습화기억능력적영향,관찰심력쇠갈여인지공능지간적상관성.설계:이실험동물위관찰대상적수궤대조관찰.단위:북경중의약대학동직문의원교육부중의내과학중점실험실기북경시중의내과학중점실험실.재료:실험우2001-11재북경중의약대학동직문의원교육부(북경시)중점실험실완성.선택구우북경유통리화실험동물기술유한공사적10～12주령건강웅성SD대서100지,체질량(200～220)g,수궤분위수술조(n=60)화가수술조(n=40).방법:수술조대서채용결찰좌관상동맥전강지적방법제작급성심기경사모형,가수술조대서재좌관상동맥전강지류치일송결,불결찰.장심전도시8개도련유심기경사양개변적존활대서작위부합조건적수술조대서(n=33),심전도몰유이상개변적미결찰존활대서작위부합조건적가수술조대서(n=25),장량조분별안시간분위10 d조、30 d조、60 d조.분별우술후10、30、60 d진행Morris수미궁실험,병측혈류동역학지표.재수술10 d조、30 d조화60 d조중,유우심력쇠갈(간칭심쇠)체력불제이무법완성전부Morris유영실험,차재실험후2 d내사망혹혈류동역학측정후사망적대서조성심쇠종말기조,기대조조위여사망대서동조적체질량상근적수술조대서.주요관찰지표:①도피대수잠복기화인지능력교호솔.②매박량、매분심배출량、심솔화심지수치,기중심지수치위주요반응심공능적지표.결과:부합조건적수술조대서33지,가수술조대서25지,진입결과분석.①혈류동역학지표측정결과:수술10 d、30 d、60 d조대서적매박량、매분심배출량、심지수균명현저우동기가수술조(P＜0.01),심쇠종말기조칙현저저우종말대조조급수술조각기대서(P＜0.01-0.05),기중수술조여동기가수술조,심쇠종말기조급종말대조조심지수비치분별위51.21%、50.58%、55.84%화33.91%,균저우60%.②Morris수미궁측험결과:수술10 d조후8차훈련대수잠복기교가수술10 d조명현연장[F(1,28)=5.997,P=0.021],수색책략방면수술10 d조적인지공능명현비가수술10 d조차(x2=8.142,P＜0.005).수술30 d조여가수술30 d조비교몰유명현변화.수술60d조여가수술60 d조대서대수잠복기몰유현저차이,수색책략방면,수술60d조총적인지능력교호솔교가수술60 d조차(x2=4.988,P＜0.05).심쇠종말기조여종말대조조대수잠복기명현연장[F(1,6)=19.567,P=0.004],수색책략방면종말기조총적인지능력교호솔교종말대조조차(x2=11.82,P＜0.01).결론:심기경사충혈성심쇠후대서적공간학습기억능력,재10 d시명현수손,재30 d시회복정상,도60d시개시출현일정정도적인지손상,재충혈성심쇠종말기시인지공능명현변차.
BACKGROUND: Chronic congestive heart failure occurs at the late stage of many heart diseases. Cognitive disability exists in chronic congestive heart failure which has been reported in most clinical studies, and measures that can successfully improve heart function can improve cognitive function.OBJECTIVE: To probe into the effect of chronic congestive heart failure after myocardial infarction on spatial learning and memory ability,and to observe the correlation between heart failure and cognitive function.DESIGN: A randomized controlled observation based on the experimental animals.SETTING: Key Laboratory of Traditional Chinese Medicine (Beijing University of Chinese Medicine), Ministry of Education.MATERIALS: This experiment was performed at the Key Laboratory of Traditional Chinese Medicine (Beijing University of Chinese Medicine),Ministry of Education. in November 2001. Totally 100 healthy male rats aged 10 to 12 weeks, weighing 200 g to 220 g purchased from Beijing Weitong Lihua Experimental animal Technical Co. Ltd were enrolled and randomly divided into the operation group (n=60) and sham-operation group (n=40).METHODS: Model rats in the operation group were infarcted by ligation of the left anterior descending coronary arteries to produce acute myocardial infarction models. Those in the sham-operation group left a loose tie without ligation. The rats that showed myocardial infarction-like changes through electrocardiogram were taken as the rats for operation (n=38), and those survived rats without abnormal changes and without ligation were taken as the sham-operation group (n=25). The two groups were subdivided into 10-day gro up, 30-day group and 60-day group. Morris water maze test was performed 10, 30 and 60 days after the operation followed by hemodynamic monitoring. Among the 10-day group, 30-day group and 60-day group, rats that did not complete the whole Morris swimming test due to heart failure with poor power and died 2 days after the operation or after hemodynamic monitoring were set as heart failure end period group.Rats in the same group as those died with similar body mass were set as the control group.MAIN OUTCOME MEASURES: ① Escape logarithm latency and the good rate if cognitive ability; ② stroke volume, cardiac output per minute,heart rate and cardiac index of hemodynamics, in which cardiac index was the main index for responding heart functionRESULTS: Totally 33 rats met the criteria in the operation group and 25rats in the sham-operation group. All the animals entered the stage of result analysis. ①Results of hymodynamic index: Stroke volume, cardiac output per minute and cardiac index of the rats in the 10-day group, 30-day group and 60-day group were significantly lower than those of the homochronous sham-operation group (P ＜ 0.01), and those indexes were significantly lower in the heart failure end period group than in the end period control group and each period of the operation (P ＜ 0.01-0.05). As compared with homochronous control group, the cardiac index in 10-day group, 30-day group, 60-day group and end period group was 51.21%,50.58%,55.84% and 33.91% respectively, and all were 60% lower than the normal value. ② Results of Morris water maze: Logarithm latency of 10-day operation group after training of 8 times was obviously longer than that of the 10-day sham-operation group [F(1,28) =5.997,P=0.021]. As for cognitive function, it was obviously worse in the 10-day operation group than that of the 10-day sham-operation group (x2=8.142 ,P ＜ 0.005). There was no significant difference between the 30-day operation group and the 30-day sham-operation group, and there was no significant difference in the logarithm latency as well between the 60-day operation group and the 60-day sham-operation group. As for searching strategies, the good rate of general cognitive ability in the 60-day operation group was worse than that in the 60-day sham-operation group (x2=4.988 ,P ＜ 0.05). Logarithm latency prolonged significantly in the heart failure end period group than in the end period control group [F( 1,6)=19.567 ,P=0.004], and the good rate of general cognitive ability was worse in the heart failure end period than that in the end period control group(x=1 1.82,P ＜ 0.01).CONCLUSION: Spatial learning and memory ability after heart failure in rats with myocardial infarction was injured obviously 10 days after the operation, and it recovered to the normal level 30 days after the operation.Cognitive injury occurred to some extent 60 days after the operation, and the cognitive function was obviously worse at the end period of congestive heart failure.
