CAJ | 학술논문

比较研究了不同浓度三氮苯类除草剂对水稻幼苗生长、叶绿素及可溶性蛋白质含量、根尖分生组织染色体结构、分生组织和叶绿体蛋白质组分的影响.结果表明,在0.1 mg /L Atrazine处理水稻后培养1周,幼苗高度由(10.8±1.7 ) cm(对照)降至 (8.2±0.7) cm,叶绿素含量由(1.07±0.013) mg/g FW(对照)降至(0 .97±0.013) mg/g FW, 可溶性蛋白由40.4 mg/g FW(对照)降至29.3 mg/g FW.Atrazin e可明显影响染色体结构,以0.01 mg/L处理水稻时,有0.68%的细胞中发现染色体凝集,0 .86%的细胞中有微核存在,该现象出现频率随Atrazine处理浓度的提高而增加.用2D-PAG E分析,在0.1 mg/L Atrazine处理后,根尖分生组织中2种新的蛋白质组分诱导产生,8种蛋白质组分消失,1种叶绿体蛋白质组分消失,3种蛋白质组分含量减少.Atrazine引起幼苗生长,叶绿素和可溶性蛋白质含量及根尖分生组织、叶绿体蛋白质组分变化的临界剂量为0 .1 mg/L,引起水稻染色体凝集和微核形成的临界剂量为0.001 mg/L.
비교연구료불동농도삼담분류제초제대수도유묘생장、협록소급가용성단백질함량、근첨분생조직염색체결구、분생조직화협록체단백질조분적영향.결과표명,재0.1 mg /L Atrazine처리수도후배양1주,유묘고도유(10.8±1.7 ) cm(대조)강지 (8.2±0.7) cm,협록소함량유(1.07±0.013) mg/g FW(대조)강지(0 .97±0.013) mg/g FW, 가용성단백유40.4 mg/g FW(대조)강지29.3 mg/g FW.Atrazin e가명현영향염색체결구,이0.01 mg/L처리수도시,유0.68%적세포중발현염색체응집,0 .86%적세포중유미핵존재,해현상출현빈솔수Atrazine처리농도적제고이증가.용2D-PAG E분석,재0.1 mg/L Atrazine처리후,근첨분생조직중2충신적단백질조분유도산생,8충단백질조분소실,1충협록체단백질조분소실,3충단백질조분함량감소.Atrazine인기유묘생장,협록소화가용성단백질함량급근첨분생조직、협록체단백질조분변화적림계제량위0 .1 mg/L,인기수도염색체응집화미핵형성적림계제량위0.001 mg/L.
In this paper, the comparative studies on the effects of triazine herbicide Atrazine on seedlings growth, contents of chlorophyll and soluble protein, chromosome structure of root meristem, protein compositions of root meristems and chloroplast have been made in Oryza sativa. The seedlings growth was obviously reduced from (10.8±1.7) cm (control) to (8.2± 0.7) cm, chlorophyll contents were decreased from (1.07±0.013) mg/g FW(control) to (0.97±0.013) mg/g FW, and soluble protein contents were decreased from 40.4 mg/g FW (control) to 29.3 mg/g FW when seedlings were treated with 0.1 mg/L Atrazine for 7 days. 0.68% cells with chromosome condensation and 0.86 % cells with micronuclei were revealed in root meristems when the root meristems were treated with 0.01 mg/L Atrazine. The frequency of chromosome condensation and micronuclei formation were increased with the increase of Atrazine concentration from 0.001 mg/L to 10 mg/L(Table 4). In 2D SDS-PAGE, 2 new protein spots were induced and 8 protein spots were lost in root meristems, 1 protein spot was lost and contents of 3 protein spots were decreased in the chloroplast when seedlings were treated with 0.1 mg/L Atrazine. The threshold concentration obviously affected seedling growth, contents of chlorophyll and soluble protein, protein compositions of root meristems and chloroplast was 0.1 mg/L. The threshold concentration induced the chromosome condensation and micronuclei formation was 0 .001 mg/L.