CAJ | 학술논문

利用细菌通用引物,对从黄土高原5个地区土壤样品以及西峰剖面26个土壤样品中提取的总DNA进行聚合酶链式反应(PCR)扩增,采用变性梯度凝胶电泳(DGGE)技术对PCR产物进行分析,以揭示黄土高原土壤中细菌群落结构的多样性.针对黄土高原土壤微生物特点进行DGGE试验条件优化,获得最佳变性梯度为40%～70%,最佳电泳时间为17 h(100 V).对不同深度土壤细菌DGGE图谱的聚类分析表明,不同深度土壤细菌呈现2种不同的群落结构,推测其成因可能与季风性气候引起的温湿环境变化及冰期有关.在夏季风加强而冬季风减弱的时期,气候温暖潮湿,风化成壤作用强烈,在DGGE图谱中表现为土壤样品条带多而密;反之,风化成壤作用较弱,在DGGE图谱中表现为条带少而疏.对洛川、西峰等5个地区土壤细菌Shannon-Weiner指数的测定结果表明,Shannon-Weiner指数受条带亮度及迁移率的影响.
이용세균통용인물,대종황토고원5개지구토양양품이급서봉부면26개토양양품중제취적총DNA진행취합매련식반응(PCR)확증,채용변성제도응효전영(DGGE)기술대PCR산물진행분석,이게시황토고원토양중세균군락결구적다양성.침대황토고원토양미생물특점진행DGGE시험조건우화,획득최가변성제도위40%～70%,최가전영시간위17 h(100 V).대불동심도토양세균DGGE도보적취류분석표명,불동심도토양세균정현2충불동적군락결구,추측기성인가능여계풍성기후인기적온습배경변화급빙기유관.재하계풍가강이동계풍감약적시기,기후온난조습,풍화성양작용강렬,재DGGE도보중표현위토양양품조대다이밀;반지,풍화성양작용교약,재DGGE도보중표현위조대소이소.대락천、서봉등5개지구토양세균Shannon-Weiner지수적측정결과표명,Shannon-Weiner지수수조대량도급천이솔적영향.
Soil samples were collected from five regions and 26 soil samples from the Xifeng Profile of the Loess Plateau.Total DNA was extracted from those samples with the aid of bacterial universal primer and amplified by means of PCR and its product analyzed with the DGGE technique to explore diversity of bacterial communities.Oriented towards the features of the microbes in the soil of the plateau,conditions of the DGGE experiment were optimized with an optimal denaturant gradient ranging between 40% and 70%,and an optimal electrophoresis duration being 17 hours (100 V).Analysis of DGGE profiles of bacteria in soils at various depths revealed that there are two different types of bacterial community structures in soils at various depths in the Loess Plateau,which is presumably attributed to changes of the warm-humid environment caused by monsoon climatic conditions,and ice age.In the age when monsoon got stronger in summers and became weak in winters,the climate was warm and humid,and weathering and soil formation were intensive,which showed multiple and dense bands in the DGGE profile,whereas conversely,weathering and soil formation are weak,which showed sparse bands in the profile.The determination of Shannon-Weiner indexes of soil bacteria in the five regions of the plateau indicated that the Shannon-Weiner indexes were influenced not only by the amount of bands,but also by brightness and moving rates of the bands