CAJ | 학술논문

目的研究原癌基因c-mos在小鼠附睾的表达及c-Mos蛋白在小鼠附睾上皮细胞的定位.方法采用半定量RT-PCR和间接免疫荧光的方法分别检测c-mos mRNA和蛋白在附睾不同区域的表达,并通过免疫电镜对c-Mos蛋白在附睾上皮细胞内进行精确定位.结果 c-mos mRNA表达量在小鼠附睾头部最高,体部最低;仅在附睾头部管腔面检测到c-Mos蛋白表达;电镜观察见c-Mos蛋白定位在附睾上皮细胞的项部胞质内.结论 c-mos原癌基因在小鼠附睾的区域特异性表达以及c-Mos蛋白在附睾上皮细胞的定位,提示c-mos基因可能在精子成熟过程中发挥调节功能.
목적 연구원암기인c-mos재소서부고적표체급c-Mos단백재소서부고상피세포적정위.방법 채용반정량RT-PCR화간접면역형광적방법 분별검측c-mos mRNA화단백재부고불동구역적표체,병통과면역전경대c-Mos단백재부고상피세포내진행정학정위.결과 c-mos mRNA표체량재소서부고두부최고,체부최저;부재부고두부관강면검측도c-Mos단백표체;전경관찰견c-Mos단백정위재부고상피세포적항부포질내.결론 c-mos원암기인재소서부고적구역특이성표체이급c-Mos단백재부고상피세포적정위,제시c-mos기인가능재정자성숙과정중발휘조절공능.
Objective To investigate the expression of proto-oncogene c-mos in mouse epididymis and the distribution of c-Mos protein in epididymal epithelial cells. Methods Semi-quantitative RT-PCR and indirect immunofluorescence were applied respectively to detect the mRNA and protein expression of c-mos in different regions of epididymis. Then the distribution of c-Mos protein in epididymal epithelial cells was observed by immune electron microscopy. Results The highest expression of c-mos mRNA in the caput epididymis and lowest in the corpus epididymis was detected. The protein expression of c-Mos was only found in the luminal surface of the caput epididymal epithelium. c-Mos protein was mainly distributed in the apical cytoplasm of the epithelial cells. Conclusion The region-specific expression pattern of c-mos gene and the localization of c-Mos protein in epididymal epithelial cells suggested that it might play a regulatory role in the process of sperm maturation.