CAJ | 학술논문

采用基于生理学的体外实验,通过模拟人体的胃和小肠环境,研究胃液pH值、胃肠中的主要消化酶对胡萝卜中滴滴涕及其代谢物(总称DDTs)生物有效性的影响及DDTs在胃肠消化液和胡萝卜基质之间的分配.结果表明:胃液pH值对DDTs的生物有效性有较大影响,在pH值为2.15时,达到最大值;在实验范围内,胃蛋白酶、胆汁盐和胰酶的浓度增大对DDTs的生物有效性没有显著影响.p, p′-DDE,p, p′-DDD和p,p′-DDT在胃和小肠中的生物有效性分别为12.4%,10.9%,10.8%和34.2%,29.1%,32.3%.DDTs在胃肠消化液和基质之间的分配表明,在消化过程中DDTs在胃肠溶液中的浓度没有达到饱和状态,在胃液和胡萝卜中的分配系数分别为0.14,0.14和0.13,在小肠液和胡萝卜中分别为0.67,0.54和0.66.
채용기우생이학적체외실험,통과모의인체적위화소장배경,연구위액pH치、위장중적주요소화매대호라복중적적체급기대사물(총칭DDTs)생물유효성적영향급DDTs재위장소화액화호라복기질지간적분배.결과표명:위액pH치대DDTs적생물유효성유교대영향,재pH치위2.15시,체도최대치;재실험범위내,위단백매、담즙염화이매적농도증대대DDTs적생물유효성몰유현저영향.p, p′-DDE,p, p′-DDD화p,p′-DDT재위화소장중적생물유효성분별위12.4%,10.9%,10.8%화34.2%,29.1%,32.3%.DDTs재위장소화액화기질지간적분배표명,재소화과정중DDTs재위장용액중적농도몰유체도포화상태,재위액화호라복중적분배계수분별위0.14,0.14화0.13,재소장액화호라복중분별위0.67,0.54화0.66.
A physiologically based in vitro test was performed by simulating human gastrointestinal tract to determine the effect of pH value and main digestive enzymes on the bioaccessibility of DDT and its metabolites (DDTs) in carrot. Distribution of DDTs between digestive juice and matrix was also studied. The results show that pH of gastric juice has a great impact on bioaccessibility of DDTs, with the maxima at the pH value of 2.15. In contrast, within the range of experimental concentrations, increasing the concentrations of pepsin, bile and pancreatin has no significant effect on bioaccessibility of DDTs. In the test, bioaccessibility of p, p′-DDE, p, p′-DDD, and p, p′-DDT were 12.4%, 10.9%, and 10.8% in stomach and 34.2%, 29.1%, and 32.3% in intestinal, respectively. Distribution of DDTs between digestive juice and matrix indicates that DDTs in the digestive solution did not reach saturation during digestion processes. Partition coefficients between the digestive solution and digested carrot were 0.14, 0.14, and 0.13 in gastric solution and 0.67, 0.54, and 0.66 in intestinal solution, respectively.