CAJ | 학술논문

[目的]对极大螺旋藻藻胆蛋白α亚基进行分析研究,为该蛋白的后续研究提供依据.[方法]用生物信息学分析方法对已经在GenBank上登录的极大螺旋藻藻胆蛋白的α亚基基因(GenBank AF441177)及其翻译的氨基酸序列进行了其组成和相关性质的分析、蛋白质信号肽预测、跨膜结构域的预测及亲水性/疏水性的测定,同时构建了分子进化树,对上述结果进行了分析和探讨.[结果]该蛋白的氨基酸组成丰富,不仅含18种必需氨基酸还含有含有甘氨酸、天冬氨酸等非必需氨基酸;对该蛋白信号肽和跨膜结构域分析表明,其属于胞内蛋白;对该蛋白的亲水性分析表明,其属于亲水性蛋白;进化树分析表明,该蛋白与节旋藻的同源性高,达99%～100%.[结论]该研究为α亚基和β亚基之间的关系和相互作用提供了一定依据.
[목적]대겁대라선조조담단백α아기진행분석연구,위해단백적후속연구제공의거.[방법]용생물신식학분석방법대이경재GenBank상등록적겁대라선조조담단백적α아기기인(GenBank AF441177)급기번역적안기산서렬진행료기조성화상관성질적분석、단백질신호태예측、과막결구역적예측급친수성/소수성적측정,동시구건료분자진화수,대상술결과진행료분석화탐토.[결과]해단백적안기산조성봉부,불부함18충필수안기산환함유함유감안산、천동안산등비필수안기산;대해단백신호태화과막결구역분석표명,기속우포내단백;대해단백적친수성분석표명,기속우친수성단백;진화수분석표명,해단백여절선조적동원성고,체99%～100%.[결론]해연구위α아기화β아기지간적관계화상호작용제공료일정의거.
[Objective] The α subunit gene of phycobiliprotein from Spirulina maxima was studied in order to provide a basis for the subsequent study of phycobiliprotein. [Method] Amino acids composition, signal peptides, hydrophobicity/hydrophilicity and trails-membrane topological structure of α subunit gene of phycobiliprotein from Spirulina maxima which registered in GenBank (GenBank AF441177) were analyzed and predicted by the tools of bioinformatic analysis. Meanwhile, phylogenetic tree was constructed based on α subunit gene of phycobiliprotein from Spirulina maxima, and its molecular evolution was also analyzed. [Result] The phycobiliprotein was rich in amino acids, which not only contained 18 kinds of essential amino acids, but also contained some non-essential amino acids like glycine, aspartic acid, etc.; Analysis on signal peptides and trails-membrane topological structure showed that the phycobiliprotein belonged to intracellular protein; Analysis on hydrophobicity/hydrophilicity showed that the phycobiliprotein belonged to hydrophilic protein; Phylogenetic analysis showed that the phycobiliprotein had a high homology with Arthrospira, which reached 99%-100%. [Conclusion] The study provided a certain reference for studying the relationship and interaction between α subunit and β subunit.