CAJ | 학술논문

目的探讨红霉素是否对鼻病毒14(RV14)介导的细胞因子产生和气道黏液高分泌有抑制作用.方法 EM预处理肺泡Ⅱ型上皮细胞A549 3h后,用RV14刺激细胞,然后采用酶联免疫吸附试验法检测细胞培养上清中细胞因子白介素8和细胞裂解液中黏液蛋白MUC5AC的浓度,并用Western blot检测磷酸化p44/42MAPK信号分子变化情况.结果红霉索明显抑制RV14介导的白介素8和MUC5AC的产生和分泌,并且对RV14介导的p44/42MAPK的激活也有抑制作用.结论红霉素可有效抑制RV14介导的细胞因子和黏液蛋白的产生和分泌,并且这种抑制作用可能是通过阻断p44/42MAPK信号分子的激活来实现的.
목적 탐토홍매소시부대비병독14(RV14)개도적세포인자산생화기도점액고분비유억제작용.방법 EM예처리폐포Ⅱ형상피세포A549 3h후,용RV14자격세포,연후채용매련면역흡부시험법검측세포배양상청중세포인자백개소8화세포렬해액중점액단백MUC5AC적농도,병용Western blot검측린산화p44/42MAPK신호분자변화정황.결과 홍매색명현억제RV14개도적백개소8화MUC5AC적산생화분비,병차대RV14개도적p44/42MAPK적격활야유억제작용.결론 홍매소가유효억제RV14개도적세포인자화점액단백적산생화분비,병차저충억제작용가능시통과조단p44/42MAPK신호분자적격활래실현적.
Objective To examine the effect of erythromycin on rhinovirus-14 (RV14)-induced cytokines and airway mucin hypersecretion. Methods A549 cells were pre-incubated with medium containing 10 μmol/L erythromycin for three hours before RV14 infection. Immunoreactive interleukin-8 (IL-8) and MUC5AC were quantitated using dual antibody ELISA kits according to the manufacturer's protocol. And activated p44/42MAPK was tested by Western blot. Results Erythromycin blocked the hypersecretion of IL-8 and MUC5AC protein, and the activation of p44/42MAPK induced by RV14 in the A549 cells.Conclusions Erythromyein may inhibit RV14-indueed IL-8 and MUC5AC hypersecretion by blocking the p44/42MAPK pathway or its upstream regulators.