CAJ | 학술논문

目的建立视神经损伤和视神经损伤复合晶状体损伤动物模型,了解2种模型损伤后不同时间闪光视觉诱发电位(F-VEP)的变化规律.方法应用液压冲击颅脑损伤仪(FPI)建立兔外伤性视神经损伤模型和视神经损伤复合晶状体损伤模型.于损伤前及损伤后1、2、4、7、10、14、21、28d行F-VEP检查并进行比较,并于上述各时间点制作视网膜切片,采用免疫组织化学法检测视网膜中巨噬细胞的数量,用焦油紫染色标记存活的视网膜神经节细胞(RGCs),对存活的RGCs进行计数.结果损伤后1d,实验组与对照组的P_(100)波隐含值和振幅降低,与损伤前相比差异均有统计学意义(P<0.05).损伤后实验组P_(100)波隐含值延长的时间与对照组相比持续的时间短(P<0.05),14d后实验组比对照组P_(100)波隐含值恢复得更快(P<0.05).实验组随着损伤时间的延长巨噬细胞数量明显增加(P<0.05),而RGCs存活数逐渐减少(P<0.05),至损伤后28d有所恢复.对照组表现出相同的变化趋势.结论应用FPI可以成功建立外伤性视神经损伤动物模型.视网膜组织病理学检测结果印证了F-VEP的检查结果.
목적 건립시신경손상화시신경손상복합정상체손상동물모형,료해2충모형손상후불동시간섬광시각유발전위(F-VEP)적변화규률.방법 응용액압충격로뇌손상의(FPI)건립토외상성시신경손상모형화시신경손상복합정상체손상모형.우손상전급손상후1、2、4、7、10、14、21、28d행F-VEP검사병진행비교,병우상술각시간점제작시망막절편,채용면역조직화학법검측시망막중거서세포적수량,용초유자염색표기존활적시망막신경절세포(RGCs),대존활적RGCs진행계수.결과 손상후1d,실험조여대조조적P_(100)파은함치화진폭강저,여손상전상비차이균유통계학의의(P<0.05).손상후실험조P_(100)파은함치연장적시간여대조조상비지속적시간단(P<0.05),14d후실험조비대조조P_(100)파은함치회복득경쾌(P<0.05).실험조수착손상시간적연장거서세포수량명현증가(P<0.05),이RGCs존활수축점감소(P<0.05),지손상후28d유소회복.대조조표현출상동적변화추세.결론 응용FPI가이성공건립외상성시신경손상동물모형.시망막조직병이학검측결과인증료F-VEP적검사결과.
Background Visual electrophysiology is a sensitive index for the evaluation of visual function.It has an important value in the assessment of traumatic optic neuropathy.Rabbit is an ideal animal model of traumatic optic neuropathy,and it is simple for the record of flash visual evoked potential(F-VEP)in rabbits.ObjectiveThe present study is to establish the animal model of traumatic optic neuropathy with or without lens injury and observe the repairing procedure using F-VEP. MethodsModels of traumatic optic neuropathy associated with lens injury were established in the right eyes and only traumatic optic neuropathy were created in the left eyes of 64 healthy SPF Chinese white rabbits using fluid percussion brain injury device(FPI).F-VEP was recorded based on the Proposal of International Visual Electrophysiology on 1,2,4,7,10,14,21,28 days after injury of optic nerves.Experimental animals were sacrificed in above time points for the histopathological examination.Macrophages were labeled by ED-1 antibody and survival retinal ganglion cells (RGCs)were stained by Nissl method.Results At the first day after injury,the latencies of P_(100) in both group were longer,and the amplitudes of P_(100) in both group were lower than before injury,showing statistically significant differences among different time points(P<0.05),but no significant difference was seen between the two groups(P>0.05).The duration of latency in traumatic optic neuropathy associated with lens injury group was shorter than that in only traumatic optic neuropathy group(P<0.05).The restore of latency in traumatic optic neuropathy associated with lens injury group was much faster than that in only traumatic optic neuropathy group(P<0.05).The numbers of macrophages were significantly increased and numbers of survival RGCs were considerably decreased with lapse of injury time (P<0.05).The abnormalities of VEP P_(100) and RGCs were obviously improved in 28 days after injury in both groups. ConclusionThis animal model can be established successfully by FPI.The result of retinal histopathological examination confirms F-VEP findings in this model.