CAJ | 학술논문

目的研究香兰素衍生物4-羟基-3,5-二甲氧基苯甲醛(VND3207)对γ射线整体照射小鼠骨髓细胞遗传损伤的防护作用.方法 BALB/c小鼠按10、50和100 mg/kg不同剂量灌胃给药,1次/d,连续5 d,最后一次给药后2 h,2 Gy60Co γ射线照射,照射后48 h观察小鼠骨髓嗜多染红细胞微核、染色体畸变和骨髓有丝分裂指数的改变.结果不同剂量(10、50和100 mg/kg)的VND3207均能有效地降低小鼠骨髓嗜多染红细胞微核率(t=2.40～4.26,P＜0.05)和染色体畸变率(t=2.36～3.52,P＜0.05),同时提高骨髓有丝分裂指数.药物保护效果与给药剂量呈依赖关系,其中100 mg/kg剂量组的微核率和染色体畸变率与单纯照射组相比,分别降低了65%和50%.结论 VND3207对60Co γ射线诱发的小鼠骨髓辐射损伤有良好的保护作用.
목적 연구향란소연생물4-간기-3,5-이갑양기분갑철(VND3207)대γ사선정체조사소서골수세포유전손상적방호작용.방법 BALB/c소서안10、50화100 mg/kg불동제량관위급약,1차/d,련속5 d,최후일차급약후2 h,2 Gy60Co γ사선조사,조사후48 h관찰소서골수기다염홍세포미핵、염색체기변화골수유사분렬지수적개변.결과 불동제량(10、50화100 mg/kg)적VND3207균능유효지강저소서골수기다염홍세포미핵솔(t=2.40～4.26,P＜0.05)화염색체기변솔(t=2.36～3.52,P＜0.05),동시제고골수유사분렬지수.약물보호효과여급약제량정의뢰관계,기중100 mg/kg제량조적미핵솔화염색체기변솔여단순조사조상비,분별강저료65%화50%.결론 VND3207대60Co γ사선유발적소서골수복사손상유량호적보호작용.
Objective To study the protection of vanillin derivative VND3207 on the cytogenetic damage of mouse bone marrow cell induced by ionizing radiation.Methods BALB/c mice were randomly divided into five groups:normal control group,2 Gy dose irradiation group,and three groups of 2 Gy irradiaiton with VND3207 protection at doses of 10,50 and 100 mg/kg,respectively.VND3207 was given by intragastric administration once a day for five days.Two hours after the last drug administration,the mice were irradiated with 2 Gy γ-rays.The changes of polychromatophilic erythroblasts micronuclei (MN),chromosome aberration (CA) and mitosis index (MI) of mouse bone marrow cells were observed at 24 and 48 h after irradiation.Results Under the protection of VND3207 at the dosages 10,50,100 mg/kg,the yields of poly-chromatophilic erythroblasts MN and CA of bone marrow cells were significantly decreased(t = 2.36-4.26,P ＜ 0.05),and the marrow cells MI remained much higher level compared with the irradiated mice without drug protection (t = 2.58,2.01,P ＜ 0.05).The radiological protection effect was drug dose-dependent,and the administration of VND3207 at the dosage of 100 mg/kg resulted in reduction by 50% and 65% in the yields of MN and CA,respectively.Conclusions VND3207 had a good protection effect of on γ-ray induced cytogentic damage of mouse bone marrow cells.