CAJ | 학술논문

目的探讨胰岛素治疗幼年大鼠溺水后应激性高血糖的疗效及其机制.方法采用溺水的方法建立应激性高血糖的大鼠动物模型.32只大鼠随机分为对照组、溺水组、空气复苏组、空气复苏联合胰岛素治疗组.空气复苏胰岛素治疗组在复苏时注射胰岛素.在溺水及复苏过程中监测大鼠血糖、胰岛素、TNF-α的含量变化,并测定骨骼肌葡萄糖摄取率.结果溺水组葡萄糖摄取率显著下降,胰岛素抵抗指数升高,产生严重的胰岛素抵抗,TNF-α急剧升高(P<0.05).空气复苏胰岛素治疗组血糖、TNF-α降低较空气复苏组显著,骨骼肌葡萄糖摄取率显著提高(P<0.05).结论积极使用胰岛素对控制溺水应激性高血糖、抑制炎症均有积极意义,可降低溺水大鼠的病死率.
목적 탐토이도소치료유년대서닉수후응격성고혈당적료효급기궤제.방법 채용닉수적방법 건립응격성고혈당적대서동물모형.32지대서수궤분위대조조、닉수조、공기복소조、공기복소연합이도소치료조.공기복소이도소치료조재복소시주사이도소.재닉수급복소과정중감측대서혈당、이도소、TNF-α적함량변화,병측정골격기포도당섭취솔.결과 닉수조포도당섭취솔현저하강,이도소저항지수승고,산생엄중적이도소저항,TNF-α급극승고(P<0.05).공기복소이도소치료조혈당、TNF-α강저교공기복소조현저,골격기포도당섭취솔현저제고(P<0.05).결론 적겁사용이도소대공제닉수응격성고혈당、억제염증균유적겁의의,가강저닉수대서적병사솔.
Objective To investigate the therapeutic effects and mechanism of insulin on stress hy-perglycemia in the drowning rats. Methods Stress hyperglycemia rat model was induced by drowning. Thir-ty-two rats were randomized into control, drowning, air resuscitation and air-insulin resuscitation groups. Insu-lin was injected into the air-insulin resuscitation rats via abdomen. The glucose,insulin, TNF-α level of serum were determined during the drowning and resuscitation. 3H-D-glucose was used to measure the glucose uptake rate (GUR) of skeletal muscles. Results Compared with those in the control group, the GUR decreased,in-sulin resistance index (HOMA-IR) increased significantly in drowning group. Compared with those in the air resuscitation group, the blood glucose and TNF-α were decreased, the GUR increased significantly in air-insu-lin resuscitation group. Conclusion Insulin therapy may decrease the blood glucose and TNF-α level, in-crease the GUR of stress hyperglycemia,and the mortality of drowning rats can be decreased.