CAJ | 학술논문

目的探讨胶原复合梯度羟基磷灰石(Col/HA)双相支架负载软骨细胞修复兔膝关节骨软骨缺损的可行性及疗效.方法构建Col/HA双相支架,将软骨细胞种植于支架培养1周,再将软骨细胞-支架复合体移植修复兔膝关节股骨髁的骨软骨缺损,并对骨软骨缺损的修复进行检测.结果光镜及扫描电镜观察显示软骨细胞在Col/HA支架中贴附良好,表型维持稳定,分泌胞外基质.大体观察和组织学检测显示,植入体内16周后实验组软骨层呈透明软骨样修复,软骨下骨缺损有新骨构建;对照组骨软骨缺损修复不良,组织学检测以纤维性组织或纤维软骨组织形成.Wakitani评分显示实验组修复组织优于对照组,差异有统计学意义(P＜0.05).结论双相Col/HA复合支架可作为骨软骨组织工程支架,负载软骨细胞可修复兔膝关节骨软骨缺损,重建关节软骨的结构和功能.
목적 탐토효원복합제도간기린회석(Col/HA)쌍상지가부재연골세포수복토슬관절골연골결손적가행성급료효.방법 구건Col/HA쌍상지가,장연골세포충식우지가배양1주,재장연골세포-지가복합체이식수복토슬관절고골과적골연골결손,병대골연골결손적수복진행검측.결과 광경급소묘전경관찰현시연골세포재Col/HA지가중첩부량호,표형유지은정,분비포외기질.대체관찰화조직학검측현시,식입체내16주후실험조연골층정투명연골양수복,연골하골결손유신골구건;대조조골연골결손수복불량,조직학검측이섬유성조직혹섬유연골조직형성.Wakitani평분현시실험조수복조직우우대조조,차이유통계학의의(P＜0.05).결론 쌍상Col/HA복합지가가작위골연골조직공정지가,부재연골세포가수복토슬관절골연골결손,중건관절연골적결구화공능.
Objective To evaluate the effectiveness and feasibility for the treatment of osteochondral defects on the femoral condyles by collagen/hydroxyapatite (Col/HA) biphasic scaffolds loaded with the cultured articular cartilage chondrocytes in rabbit knee joint. Methods Biphasic scaffolds were constructed with collagen complex gradient hydroxyapatite. The chondrocytes of third generation were embedded in the biphasic scaffolds' collagen. After culture for one week in vitro, the complexes of chondrocytesscaffold were transplanted to repair the articular osteochondral defects of the femoral condyle of rabbit knees ( the defects were 4 mm in diameter, 3.5 mm in depth, extending down to the subchondral bone). Healing of defects was assessed by gross and histological examination. Results Biphasic collagen/hydroxyapatite scaffold had good hydrophilia and permeability. Light microscopy and scanning electron microscopy revealed that chondrocytes attaching to the wall of microholes of the scaffold had largely maintained a rounded morphology and could secrete the extracellular matrix on the porous scaffold. Gross and light microscopic examination showed that the treatment of simple Col/HA scaffold improved defect filling compared with that left untreated, while the regenerated tissue was mainly fibrocartilage and showed little bone formation.When implanted with Col/HA scaffold combined with chondrocytes, most of the defects were filled with a well-established layer of cartilage tissue with new bone formation. Wakitani scoring showed that experimental group was superior to control groups in 16 weeks with the difference being significant between them (P ＜0.05). Conclusion The novel biphasic collagen/hydroxyapatite scaffold will possibly be an ideal scaffold for osteochondral tissue engineering. It may induce articular osteochondral regeneration to repair the defects of articular osteochondral in vivo after loaded with chondrocytes.