CAJ | 학술논문

目的观察手汗症伴腋窝多汗患者腋窝汗腺中水通道蛋白(AQP)的表达,探讨其与手汗症伴腋窝多汗的发病机制的关系.方法应用苏木素-伊红(HE)染色对34例实验组和8例对照组的腋窝汗腺进行染色,在光镜下分别计算其腋窝汗腺数目,并进行比较;应用免疫组织化学方法对上述两组腋窝汗腺进行AQP1、AQP3和AQP5蛋白检测,并进行对照研究.结果两组中腋窝汗腺数目差异无统计学意义(Z=-1.506,P>0.05);两组腋窝汗腺中均无AQP1、AQP3表达,AQP5在两组均有表达且在实验组中表达显著增强(Z=-3.523,P<0.05).结论 AQP1、AQP3不参与人体腋窝汗腺分泌;AQP5可能是参与人体腋窝汗腺分泌的主要水通道蛋白之一,其表达增强很可能是手汗症伴腋窝多汗患者的发病机制之一.
목적 관찰수한증반액와다한환자액와한선중수통도단백(AQP)적표체,탐토기여수한증반액와다한적발병궤제적관계.방법 응용소목소-이홍(HE)염색대34례실험조화8례대조조적액와한선진행염색,재광경하분별계산기액와한선수목,병진행비교;응용면역조직화학방법대상술량조액와한선진행AQP1、AQP3화AQP5단백검측,병진행대조연구.결과 량조중액와한선수목차이무통계학의의(Z=-1.506,P>0.05);량조액와한선중균무AQP1、AQP3표체,AQP5재량조균유표체차재실험조중표체현저증강(Z=-3.523,P<0.05).결론 AQP1、AQP3불삼여인체액와한선분비;AQP5가능시삼여인체액와한선분비적주요수통도단백지일,기표체증강흔가능시수한증반액와다한환자적발병궤제지일.
Objective To examine the expression of aquaporin (AQP) in axillary sweat glands of palmar hyperhidrosis patients with axillary hyperhidrosis and investigate its relationship with the pathogene-sis of palmar hyperhidrosis patients with axillary hyperhidrosis. Methods Axillary sweat glands were obtained in 34 cases of hyperhidrosis and 8 cases of non-hyperhidrosis as control group. The number of axillary sweat glands was calculated by using HE staining and the expression of aquaporins (AQP1, AQP3, AQP5) was detected by using immunochemical method respectively. The comparison was made between hyperhidrosis group and control group. Results The number of axillary sweat glands in two groups had no significant difference in statistics (Z=- 1.506, P>0. 05 ). Both groups had the expression of AQP5, and AQP1, AQP3 were undetectable; The AQP5 expression of axillary sweat glands in hyperhidmsis cases was significantly increased as compared with that in control group (Z=- 3. 523, P<0.05). Conclusion AQP5 involves secretion of human axillary sweat glands. The increase of AQP5 is probably one of pathogenic mechanisms for palmar hyperhidrosis patients with axillary hyperhidrosis.