CAJ | 학술논문

目的观察低温保存不同时间大鼠带瓣血管活性及bax和bcl-2基因的表达,探讨基因检测是否能成为带瓣血管活性指标.方法 Wistar大鼠80只,体质量250～350 g,分为新鲜组A组,-196 ℃液氮冻存3、6、9个月为B、C、D 3组.检测细胞结构、糖代谢及bax、bcl-2基因的变化.结果大鼠带瓣血管的各组葡萄糖代谢率测定,A组(4.365±0.784)与B(4.383±0.548)、C组(4.446±0.608)、D组(4.090±0.657)差异无统计学意义(P＞0.05);bax基因的表达分别为20%、45%、60%和85%,差异有统计学意义(P＜0.05);bcl-2基因表达分别25%、30%、20%和35%,差异无统计学意义(P＞0.05).结论经冻存后的大鼠带瓣血管活性良好,提示bax基因可作为检测带瓣血管活性的指标,bcl-2的表达或高表达可能是细胞能耐受冻存打击的原因之一.
목적 관찰저온보존불동시간대서대판혈관활성급bax화bcl-2기인적표체,탐토기인검측시부능성위대판혈관활성지표.방법 Wistar대서80지,체질량250～350 g,분위신선조A조,-196 ℃액담동존3、6、9개월위B、C、D 3조.검측세포결구、당대사급bax、bcl-2기인적변화.결과 대서대판혈관적각조포도당대사솔측정,A조(4.365±0.784)여B(4.383±0.548)、C조(4.446±0.608)、D조(4.090±0.657)차이무통계학의의(P＞0.05);bax기인적표체분별위20%、45%、60%화85%,차이유통계학의의(P＜0.05);bcl-2기인표체분별25%、30%、20%화35%,차이무통계학의의(P＞0.05).결론 경동존후적대서대판혈관활성량호,제시bax기인가작위검측대판혈관활성적지표,bcl-2적표체혹고표체가능시세포능내수동존타격적원인지일.
Objective To study the expression of bax and bcl-2 genes and activity of valved conduit in rats by cryopreservation for different lengths, and to probe whether genetic detection will become one of the indexes of valved conduit activity. Methods Eighty Wistar rats were divided into four groups: 20cases of fresh blood vessels ( group A ), 20 cases of frozen vessels for 3 months ( group B ), 20 for 6months ( group C), and 20 for 9 months ( group D). The changes in cellular structure, gluoce metabolism and the expression of bax and bcl-2 proteins were observed. Results The glucose metabolism rate of the rate valved conduit in groups A, B, C and D was 4. 365 ± 0. 784, 4. 383 ± 0. 548, 4. 446 ± 0. 608, and 4. 090 ± 0. 657 respectively, with the difference being not significant ( P ＞ 0. 05 ). The positive expression rate of bax protein in groups A, B, C and D was 20%, 45%, 60% and 85% respectively (P＜0. 05).The positive expression rate of bcl-2 in groups A, B, C and D was 25%, 30%, 20% and 35% respectively ( P ＞ 0. 05 ). Conclusion The frozen rat valved vascular showed good activity. bax gene can be regarded as one of the indicators for detecting valved vessel activity. The expression or over-expression of bcl-2gene may be one of the reasons why the cells can tolerate the frozen preservation.