中华消化杂志
中華消化雜誌
중화소화잡지
Chinese Journal of Digestion
2010年
7期
465-469
,共5页
张振%王宝香%周诗琼%鲍连生%刘冰波
張振%王寶香%週詩瓊%鮑連生%劉冰波
장진%왕보향%주시경%포련생%류빙파
轮状病毒%嗜酸乳杆菌%乳鼠%腹泻%肠黏膜
輪狀病毒%嗜痠乳桿菌%乳鼠%腹瀉%腸黏膜
륜상병독%기산유간균%유서%복사%장점막
Rotavirus%Lactobacillus acidophilus%Neonatal mice%Diarrhea%Intestinal mucosa
目的 观察嗜酸乳杆菌(L.acidophilus)对乳鼠感染人轮状病毒(HRV)的可能预防和治疗作用.方法 将60只昆明种乳鼠随母鼠按窝随机均分为4组,给4 d龄的乳鼠灌服HRV作为HRV感染组,灌服HRV的前和后灌服嗜酸乳杆菌作为嗜酸乳杆菌预处理组和治疗组,正常对照组灌服同剂量最低限度必要成分(MEM)Eagle细胞培养液.观察各组乳鼠的临床症状、肠黏膜病理变化;酶联免疫吸附法检测各组乳鼠粪便HRV抗原;免疫荧光法检测HRV抗原在各组乳鼠小肠组织中的表达.结果 对照组乳鼠未出现腹泻.预处理组和治疗组乳鼠的腹泻程度、腹泻持续天数、HRV阳性天数和死亡率均低于HRV感染组;其肠黏膜的病变程度及HRV抗原表达也明显弱于HRV感染组.结论 嗜酸乳杆菌对乳鼠感染人轮状病毒有预防和治疗作用.
目的 觀察嗜痠乳桿菌(L.acidophilus)對乳鼠感染人輪狀病毒(HRV)的可能預防和治療作用.方法 將60隻昆明種乳鼠隨母鼠按窩隨機均分為4組,給4 d齡的乳鼠灌服HRV作為HRV感染組,灌服HRV的前和後灌服嗜痠乳桿菌作為嗜痠乳桿菌預處理組和治療組,正常對照組灌服同劑量最低限度必要成分(MEM)Eagle細胞培養液.觀察各組乳鼠的臨床癥狀、腸黏膜病理變化;酶聯免疫吸附法檢測各組乳鼠糞便HRV抗原;免疫熒光法檢測HRV抗原在各組乳鼠小腸組織中的錶達.結果 對照組乳鼠未齣現腹瀉.預處理組和治療組乳鼠的腹瀉程度、腹瀉持續天數、HRV暘性天數和死亡率均低于HRV感染組;其腸黏膜的病變程度及HRV抗原錶達也明顯弱于HRV感染組.結論 嗜痠乳桿菌對乳鼠感染人輪狀病毒有預防和治療作用.
목적 관찰기산유간균(L.acidophilus)대유서감염인륜상병독(HRV)적가능예방화치료작용.방법 장60지곤명충유서수모서안와수궤균분위4조,급4 d령적유서관복HRV작위HRV감염조,관복HRV적전화후관복기산유간균작위기산유간균예처리조화치료조,정상대조조관복동제량최저한도필요성분(MEM)Eagle세포배양액.관찰각조유서적림상증상、장점막병리변화;매련면역흡부법검측각조유서분편HRV항원;면역형광법검측HRV항원재각조유서소장조직중적표체.결과 대조조유서미출현복사.예처리조화치료조유서적복사정도、복사지속천수、HRV양성천수화사망솔균저우HRV감염조;기장점막적병변정도급HRV항원표체야명현약우HRV감염조.결론 기산유간균대유서감염인륜상병독유예방화치료작용.
Objective To investigate the potential effect of Lactobacillus acidophilus (L. acidophilus) on prevention and treatment of neonatal mice infected with human rotavirus (HRV). Methods Sixty 4-day-old kunming mice were randomly divided into control group. HRV infected group, L. acidophilus pretreated group (treated before HRV infection ) and L. acidophilus treated group(treated after HRV infection). The manifestation and pathological changes in small intestine of neonatal mice were observed. The HRV antigen in the feces and intestines were measured by ELISA and fluorescent-focus assay, respectively. Results The severity and duration of diarrhea as well as mortality in L. acidophilus pretreated group and treated group were lower than those in HRV infected group. The duration of HRV-antigens shedding following infection was considerably prolonged in HRV infected group compared to that in L. acidophilus pretreated group and treated group. Furthermore, decreased expression of HRV antigen and little pathological changes in intestinal mucosa were found in L. acidophilus pretreated group and treated group when compared with HRV infected group. Conclusion L. acidophilus may be used as an alternative approach for the prevention and treatment of neonatal mice infected with HRV.