分子植物育种
分子植物育種
분자식물육충
MOLECULAR PLANT BREEDING
2006年
4期
506-512
,共7页
张向军%陈英之%韦燕萍%吕维莉%廖惠红%刘宇锋%杨新庆%李小勇%杨朗%李丽淑%李容柏
張嚮軍%陳英之%韋燕萍%呂維莉%廖惠紅%劉宇鋒%楊新慶%李小勇%楊朗%李麗淑%李容柏
장향군%진영지%위연평%려유리%료혜홍%류우봉%양신경%리소용%양랑%리려숙%리용백
水稻%杂交不育%SSR标记%广亲和%S-5n
水稻%雜交不育%SSR標記%廣親和%S-5n
수도%잡교불육%SSR표기%엄친화%S-5n
Rice%Hybrid sterility%SSR marker%Wide compatibility%S-5n
广亲和(WC)是水稻(Oryza sativa L.)亚种间杂种优势利用的重要遗传工具.本研究对我国南方大面积推广应用的亚种间两系杂交稻亲本培矮64S的WC基因S-5n进行分子标记定位.从培矮64S//T8/秋光三交F1中选出由263个高育和低育极端类型组成的标记群体,选用来源于Cornell SSR连锁图的20个标记和根据GenBank数据库公布的序列合成的9个SSR标记,通过BSA(分离群体分析)法对标记群体进行分析,该S-5位点精确定位于第6染色体上,距SSR标记GXR6和RM276的距离只有0.2cM.培矮64S的S-5n对育性贡献可使小穗结实率从平均43.5%提高到77.5%,贡献率达34.0%,表明它是一个很强的控制广亲和特性的主效基因.本研究所获得的这些紧密连锁标记对分子标记辅助选择培育广亲和水稻品种和基因克隆具有重要的利用价值.
廣親和(WC)是水稻(Oryza sativa L.)亞種間雜種優勢利用的重要遺傳工具.本研究對我國南方大麵積推廣應用的亞種間兩繫雜交稻親本培矮64S的WC基因S-5n進行分子標記定位.從培矮64S//T8/鞦光三交F1中選齣由263箇高育和低育極耑類型組成的標記群體,選用來源于Cornell SSR連鎖圖的20箇標記和根據GenBank數據庫公佈的序列閤成的9箇SSR標記,通過BSA(分離群體分析)法對標記群體進行分析,該S-5位點精確定位于第6染色體上,距SSR標記GXR6和RM276的距離隻有0.2cM.培矮64S的S-5n對育性貢獻可使小穗結實率從平均43.5%提高到77.5%,貢獻率達34.0%,錶明它是一箇很彊的控製廣親和特性的主效基因.本研究所穫得的這些緊密連鎖標記對分子標記輔助選擇培育廣親和水稻品種和基因剋隆具有重要的利用價值.
엄친화(WC)시수도(Oryza sativa L.)아충간잡충우세이용적중요유전공구.본연구대아국남방대면적추엄응용적아충간량계잡교도친본배왜64S적WC기인S-5n진행분자표기정위.종배왜64S//T8/추광삼교F1중선출유263개고육화저육겁단류형조성적표기군체,선용래원우Cornell SSR련쇄도적20개표기화근거GenBank수거고공포적서렬합성적9개SSR표기,통과BSA(분리군체분석)법대표기군체진행분석,해S-5위점정학정위우제6염색체상,거SSR표기GXR6화RM276적거리지유0.2cM.배왜64S적S-5n대육성공헌가사소수결실솔종평균43.5%제고도77.5%,공헌솔체34.0%,표명타시일개흔강적공제엄친화특성적주효기인.본연구소획득적저사긴밀련쇄표기대분자표기보조선택배육엄친화수도품충화기인극륭구유중요적이용개치.
Wide compatibility (WC) is an important genetic tool for exploitation of inter-specific heterosis in rice (Oryza sativa L.). In this study, Pei' ai 64S, one of the most popular thermosensitive genic male sterile (TGMS) line widely used for subspecific hybrid rice breeding program in South China, was studied to locate its WC allele S-5n by exploiting SSR markers. A mapping population containing 263 extreme fertile and sterile plants was developed from a three-way cross (Pei'ai 64S/T8//Akihikari). Surrounding the S-5 locus, 29 SSR (simple sequence repeat)markers of which 20 derived from Cornell SSR linkage map and 9 developed using sequences from GenBank database, were employed for the molecular assay. The bulked segregant analysis was performed to tag fine location of the locus. This S-5 locus was mapped on chromosome 6 approximately 0.2cM from both of the SSR markers GXR6 and RM276. The contribution of the S-5n allele to spikelet fertility reached as high as 34.0% in Pei' ai 64S,increasing from 43.5% to 77.5%, indicating it was a main effect gene controlling the wide compatibility. The tight linkage markers would be very useful for efficient marker-assisted selection of rice WC varieties and map-based cloning of the gene.
