CAJ | 학술논문

目的了解广州地区14岁以下急性呼吸道患儿人类偏肺病毒(humanmetapneumovirus,hMPV)的感染情况,分析广州地区儿童感染hMPV的流行病学特征及临床特征,为hMPV的预防和治疗提供一些依据.方法 2006年9月至2008年8月,在广州某医院的儿科门诊及住院部的急性呼吸道患儿中采集521份鼻咽拭子标本,提取总核酸,用逆转录聚合酶链反应(RT-PER)扩增hMPV的核蛋白(N)基因的213个核苷酸片段,对16份强阳性的扩增产物进行测序和序列分析,同时对采集病例的流行病学资料进行统计分析.结果 521份标本用RT-PCR方法检测后,共有39份标本为N基因阳性,检出率为7.49%,hMPV发病高峰期在10月和4月份,对N基因进行序列测序与分析,结果与北京株BJ1897N基因的同源性高达99%,与泰国AY550156株N基因的同源性高达97%,基因型别以B型为主.结论广州地区的儿童急性呼吸道感染中hMPV感染是原因之一,且以6岁以下的儿童感染为主,但不同性别的感染率差异无统计学意义,感染hMPV的临床症状主要表现为:高热、咳嗽、头痛、咽痛、卡他症状、肺部影像学改变等,其中大部分患者表现有高热和咳嗽,且有41.03%的hMPV患者合并感染其他呼吸道病毒.
목적 료해엄주지구14세이하급성호흡도환인인류편폐병독(humanmetapneumovirus,hMPV)적감염정황,분석엄주지구인동감염hMPV적류행병학특정급림상특정,위hMPV적예방화치료제공일사의거.방법 2006년9월지2008년8월,재엄주모의원적인과문진급주원부적급성호흡도환인중채집521빈비인식자표본,제취총핵산,용역전록취합매련반응(RT-PER)확증hMPV적핵단백(N)기인적213개핵감산편단,대16빈강양성적확증산물진행측서화서렬분석,동시대채집병례적류행병학자료진행통계분석.결과 521빈표본용RT-PCR방법검측후,공유39빈표본위N기인양성,검출솔위7.49%,hMPV발병고봉기재10월화4월빈,대N기인진행서렬측서여분석,결과여북경주BJ1897N기인적동원성고체99%,여태국AY550156주N기인적동원성고체97%,기인형별이B형위주.결론 엄주지구적인동급성호흡도감염중hMPV감염시원인지일,차이6세이하적인동감염위주,단불동성별적감염솔차이무통계학의의,감염hMPV적림상증상주요표현위:고열、해수、두통、인통、잡타증상、폐부영상학개변등,기중대부분환자표현유고열화해수,차유41.03%적hMPV환자합병감염기타호흡도병독.
Objective To find out the status of human metapneumovirus (hMPV) infection in children under 14 years old with acute respiratory tract infections (ARI) in Guangzhou, analyze the epidemiology and clinical characteristics among the hMPV-infected children, and provide some basis for research of hMPV. Methods All 521 throat and pharyngeal swabs were collected among the children with acute respiratory tract infections in outpatient departments and those admitted to the wards from September 2006 to August 2008. Then total nucleic acid was extracted from respiratory specimens. The 213 nucleosides of nucleoprotein gene were detected by RT-PCR and 16 strong positive samples were picked to compare with the sequence of hMPV in GenBank after the sequence of the amplification products were determined. Then applied statistical analysis to the data of the collected patients. Results All 521 samples were detected by RT-PCR,and confirmed that N gene was positive in 39 samples with a detection rate of 7.49% ,and the peak time was in October and April. The 16 amplification products were compared by using the analysis of gene sequence. The nucleocapsid protein (N) gene similarity to BJ1897 of Beijing was up to 99%, and to AY550156 of Thailand was up to 97%, genotype B was the most common genotype. Conclusion There existed hMPV infection in children acute respiratory system diseases in Guangzhou areas, in which the children under the age of 6 years were accounted for the main group, however there was no difference in gender. The main symptoms of the patients with hMPV infection were high fever and cough symptom of catarrh. Co-infections other than respiratory virus with hMPV were detected as 41.03% of positive samples.