中华外科杂志
中華外科雜誌
중화외과잡지
CHINESE JOURNAL OF SURGERY
2012年
8期
728-731
,共4页
盛伟伟%周建平%孔凡民%李昱骥%董明
盛偉偉%週建平%孔凡民%李昱驥%董明
성위위%주건평%공범민%리욱기%동명
胰腺肿瘤%癌,导管%碳酸酐酶Ⅱ%免疫组织化学%印迹法,蛋白质%逆转录聚合酶链反应
胰腺腫瘤%癌,導管%碳痠酐酶Ⅱ%免疫組織化學%印跡法,蛋白質%逆轉錄聚閤酶鏈反應
이선종류%암,도관%탄산항매Ⅱ%면역조직화학%인적법,단백질%역전록취합매련반응
Pancreatic neoplasms%Carcinoma,ductal%Carbonic anhydrase Ⅱ%Immunohistochemistry%Blotting,Western%Polymerase chain,reaction
目的 探讨胰腺浸润性导管癌(IDC)中碳酸酐酶Ⅱ(CAⅡ)蛋白和mRNA表达水平及其临床病理学意义.方法 应用免疫组化方法检测33例胰腺IDC组织及配对的癌旁组织石蜡标本CAⅡ蛋白表达水平,观察其与患者临床病理学参数的关系；再用Western blot和逆转录聚合酶链式反应RT-PCR检测12例冷冻保存的新鲜配对IDC组织、癌旁组织中CAⅡ蛋白和mRNA表达水平.结果 免疫组化结果显示:CAⅡ在11例胰腺IDC中呈高表达(33.3％),明显低于配对癌旁组织的表达水平(72.7％,t =6.275,P=0.000).CAⅡ的表达与胰腺IDC的发生部位(x2=0.992,P=0.319)、分化程度(x2=0.866,P=0.352)、TNM分期(x2=1.210,P=0.271)和淋巴结转移(x2=0.798,P=0.372)无明显相关性,但与患者预后的相关性接近统计学意义(x2=3.233,P=0.072).高表达CAⅡ的胰腺IDC患者中位生存时间为540 d,低表达CAⅡ者为320 d.RT-PCR和Western blot结果显示,CAⅡ在胰腺IDC中的蛋白和mRNA表达均低于配对癌旁组织(t=3.399,P=0.006；t =2.281,P =0.043).结论 CAⅡ在胰腺IDC中表达下调,并可能与患者预后相关.
目的 探討胰腺浸潤性導管癌(IDC)中碳痠酐酶Ⅱ(CAⅡ)蛋白和mRNA錶達水平及其臨床病理學意義.方法 應用免疫組化方法檢測33例胰腺IDC組織及配對的癌徬組織石蠟標本CAⅡ蛋白錶達水平,觀察其與患者臨床病理學參數的關繫；再用Western blot和逆轉錄聚閤酶鏈式反應RT-PCR檢測12例冷凍保存的新鮮配對IDC組織、癌徬組織中CAⅡ蛋白和mRNA錶達水平.結果 免疫組化結果顯示:CAⅡ在11例胰腺IDC中呈高錶達(33.3％),明顯低于配對癌徬組織的錶達水平(72.7％,t =6.275,P=0.000).CAⅡ的錶達與胰腺IDC的髮生部位(x2=0.992,P=0.319)、分化程度(x2=0.866,P=0.352)、TNM分期(x2=1.210,P=0.271)和淋巴結轉移(x2=0.798,P=0.372)無明顯相關性,但與患者預後的相關性接近統計學意義(x2=3.233,P=0.072).高錶達CAⅡ的胰腺IDC患者中位生存時間為540 d,低錶達CAⅡ者為320 d.RT-PCR和Western blot結果顯示,CAⅡ在胰腺IDC中的蛋白和mRNA錶達均低于配對癌徬組織(t=3.399,P=0.006；t =2.281,P =0.043).結論 CAⅡ在胰腺IDC中錶達下調,併可能與患者預後相關.
목적 탐토이선침윤성도관암(IDC)중탄산항매Ⅱ(CAⅡ)단백화mRNA표체수평급기림상병이학의의.방법 응용면역조화방법검측33례이선IDC조직급배대적암방조직석사표본CAⅡ단백표체수평,관찰기여환자림상병이학삼수적관계；재용Western blot화역전록취합매련식반응RT-PCR검측12례냉동보존적신선배대IDC조직、암방조직중CAⅡ단백화mRNA표체수평.결과 면역조화결과현시:CAⅡ재11례이선IDC중정고표체(33.3％),명현저우배대암방조직적표체수평(72.7％,t =6.275,P=0.000).CAⅡ적표체여이선IDC적발생부위(x2=0.992,P=0.319)、분화정도(x2=0.866,P=0.352)、TNM분기(x2=1.210,P=0.271)화림파결전이(x2=0.798,P=0.372)무명현상관성,단여환자예후적상관성접근통계학의의(x2=3.233,P=0.072).고표체CAⅡ적이선IDC환자중위생존시간위540 d,저표체CAⅡ자위320 d.RT-PCR화Western blot결과현시,CAⅡ재이선IDC중적단백화mRNA표체균저우배대암방조직(t=3.399,P=0.006；t =2.281,P =0.043).결론 CAⅡ재이선IDC중표체하조,병가능여환자예후상관.
Objective To study the clinicopathological significance of the expression of carbonic anhvdrase (CA) Ⅱ protein and mRNA in primary invasive ductal cancer (IDC) of human pancreas.Methods The expression of CA Ⅱ protein in 33 paired paraffin embedded IDC specimens of the pancreas and paired adjacent non-cancerous pancreatic tissues was detected by immunohistochemistry. Western blot and reverse transcription polymerase chain reaction ( RT-PCR ) were used to examine the expression of CA Ⅱ protein and mRNA level in 12 paired fresh IDC specimens of the pancreas and adjuvant non-cancerous pancreatic tissues. The relationship between the protein expression and clinicopathological features was analyzed.Results Overexpression of CA Ⅱ protein was shown in 11 cases of pancreatic IDC tissues ( 33.3％,11/33 ),which was much lower than that in paired non-cancerous pancreatic tissues ( 72.7％,t =6.275,P =0.000).The expression of CA Ⅱ protein had no correlation with tumor position ( x2 =0.992,P =0.319 ),differentiation ( x2 =0.866,P =0.352 ),TN M stage ( x2 =1.210,P =0.271 ) and Lymph node metastasis (x2 =0.798,P =0.372),but had bordering statistic sig with the prognosis of the patients (x2=3.233,P =0.072).The median survival time in the patients with high expression of CA Ⅱ protein was 540 days,while that in the patients with low expression was 320 days.The expression of CA Ⅱ protein and mRNA was lower in IDC than that in paired non-cancerous pancreatic tissues detected by Western blot and RT-PCR respectively (t=3.399,P=0.006; t=2.281,P=0.043).Conclusion CAⅡ is down regulated in pancreatic IDC and might be relative with the prognosis.
