中华眼科杂志
中華眼科雜誌
중화안과잡지
Chinese Journal of Ophthalmology
2001年
3期
197-199
,共3页
基因,bcl-2%增殖细胞核抗原%晶体%上皮细胞
基因,bcl-2%增殖細胞覈抗原%晶體%上皮細胞
기인,bcl-2%증식세포핵항원%정체%상피세포
目的观察正常胚胎、儿童和老年人晶状体上皮细胞中bcl-2基因和增殖细胞核抗原(proliferating cell nuclear antigen,PCNA)的表达状况,探讨其与晶状体上皮细胞增殖潜能的关系。方法采用免疫组织化学染色法光镜下检测胚胎组(胎龄5~10个月)、儿童组(7个月至13岁)及老年组(>50岁)晶状体上皮细胞中bcl-2基因和PCNA的表达状况。结果 bcl-2基因和PCNA在胚胎组和儿童组晶状体上皮细胞中表达的阳性率显著高于老年组(P<0.01),且以晶状体赤道部明显。结论晶状体上皮细胞的增殖潜能及后发性白内障的发生与晶状体上皮细胞内bcl-2基因及PCNA的表达有关。
目的觀察正常胚胎、兒童和老年人晶狀體上皮細胞中bcl-2基因和增殖細胞覈抗原(proliferating cell nuclear antigen,PCNA)的錶達狀況,探討其與晶狀體上皮細胞增殖潛能的關繫。方法採用免疫組織化學染色法光鏡下檢測胚胎組(胎齡5~10箇月)、兒童組(7箇月至13歲)及老年組(>50歲)晶狀體上皮細胞中bcl-2基因和PCNA的錶達狀況。結果 bcl-2基因和PCNA在胚胎組和兒童組晶狀體上皮細胞中錶達的暘性率顯著高于老年組(P<0.01),且以晶狀體赤道部明顯。結論晶狀體上皮細胞的增殖潛能及後髮性白內障的髮生與晶狀體上皮細胞內bcl-2基因及PCNA的錶達有關。
목적관찰정상배태、인동화노년인정상체상피세포중bcl-2기인화증식세포핵항원(proliferating cell nuclear antigen,PCNA)적표체상황,탐토기여정상체상피세포증식잠능적관계。방법채용면역조직화학염색법광경하검측배태조(태령5~10개월)、인동조(7개월지13세)급노년조(>50세)정상체상피세포중bcl-2기인화PCNA적표체상황。결과 bcl-2기인화PCNA재배태조화인동조정상체상피세포중표체적양성솔현저고우노년조(P<0.01),차이정상체적도부명현。결론정상체상피세포적증식잠능급후발성백내장적발생여정상체상피세포내bcl-2기인급PCNA적표체유관。
Objective To investigate the characteristics of bcl-2 (gene inhibiting apoptosis) and proliferating cell nuclear antigen (PCNA) expression and their relation to the proliferation of lens epithelium. Methods The expression of bcl-2 and PCNA were detected by immunohistochemical streptavidin-peroxidase (SP) method. Results The expression of bcl-2 and PCNA in the lens epithelium of fetus and children was higher than that of the elderly, especially in the region of the lens equator. Conclusion The over-expression of bcl-2 and PCNA in the lens epithelium of fetus and children suggests that bcl-2 and PCNA might be related to the development of cataract.