中国医师杂志
中國醫師雜誌
중국의사잡지
JOURNAL OF CHINESE PHYSICIAN
2012年
4期
445-448
,共4页
膀胱肿瘤/病理学/代谢%组织相容性抗原Ⅰ类/代谢%NF-κB/代谢%肿瘤抑制蛋白质p53/代谢
膀胱腫瘤/病理學/代謝%組織相容性抗原Ⅰ類/代謝%NF-κB/代謝%腫瘤抑製蛋白質p53/代謝
방광종류/병이학/대사%조직상용성항원Ⅰ류/대사%NF-κB/대사%종류억제단백질p53/대사
Urinary bladder neoplasms/pathology/metabolism%Histocompatibility antigens class I/metabolism%NF-kappa B/metabolism%Tumor suppressor protein p53/metabolism
目的 探讨膀胱尿路上皮癌中MHC Ⅰ类链相关蛋白A(MHC classⅠ chain-related A,MICA)的表达,以及与核因子-κB(nuclear factor-κB,NF-κB)和p53的相互关系,为研究膀胱癌组织中MICA蛋白的表达机制提供组织学依据.方法 用免疫组化方法检测75例膀胱尿路上皮癌及15例正常膀胱黏膜中MICA、NF-κB和p53蛋白表达,对MICA、NF-κB和p53在正常膀胱黏膜、浸润和非浸润膀胱癌中的表达进行统计学分析.结果 (1)MICA、NF-κB和p53蛋白在膀胱癌的表达率分别为48.0％、85.3％和49.3％,均显著高于正常膀胱黏膜(P＜0.05).MICA蛋白在浸润性膀胱癌的表达低于非浸润性膀胱癌(P＜0.05).(2)膀胱癌组织中MICA与NF-κB蛋白表达呈正相关(r=0.256,P=0.027),而MICA和p53蛋白的表达呈负相关(r=-0.23,P=0.047).结论 膀胱癌中MICA蛋白常表达上调,可作为膀胱癌的肿瘤相关抗原；NF-κB通路可能参与MICA的表达调控；p53通路可能不参与膀胱尿路上皮恶性转化过程中MICA蛋白的表达.
目的 探討膀胱尿路上皮癌中MHC Ⅰ類鏈相關蛋白A(MHC classⅠ chain-related A,MICA)的錶達,以及與覈因子-κB(nuclear factor-κB,NF-κB)和p53的相互關繫,為研究膀胱癌組織中MICA蛋白的錶達機製提供組織學依據.方法 用免疫組化方法檢測75例膀胱尿路上皮癌及15例正常膀胱黏膜中MICA、NF-κB和p53蛋白錶達,對MICA、NF-κB和p53在正常膀胱黏膜、浸潤和非浸潤膀胱癌中的錶達進行統計學分析.結果 (1)MICA、NF-κB和p53蛋白在膀胱癌的錶達率分彆為48.0％、85.3％和49.3％,均顯著高于正常膀胱黏膜(P＜0.05).MICA蛋白在浸潤性膀胱癌的錶達低于非浸潤性膀胱癌(P＜0.05).(2)膀胱癌組織中MICA與NF-κB蛋白錶達呈正相關(r=0.256,P=0.027),而MICA和p53蛋白的錶達呈負相關(r=-0.23,P=0.047).結論 膀胱癌中MICA蛋白常錶達上調,可作為膀胱癌的腫瘤相關抗原；NF-κB通路可能參與MICA的錶達調控；p53通路可能不參與膀胱尿路上皮噁性轉化過程中MICA蛋白的錶達.
목적 탐토방광뇨로상피암중MHC Ⅰ류련상관단백A(MHC classⅠ chain-related A,MICA)적표체,이급여핵인자-κB(nuclear factor-κB,NF-κB)화p53적상호관계,위연구방광암조직중MICA단백적표체궤제제공조직학의거.방법 용면역조화방법검측75례방광뇨로상피암급15례정상방광점막중MICA、NF-κB화p53단백표체,대MICA、NF-κB화p53재정상방광점막、침윤화비침윤방광암중적표체진행통계학분석.결과 (1)MICA、NF-κB화p53단백재방광암적표체솔분별위48.0％、85.3％화49.3％,균현저고우정상방광점막(P＜0.05).MICA단백재침윤성방광암적표체저우비침윤성방광암(P＜0.05).(2)방광암조직중MICA여NF-κB단백표체정정상관(r=0.256,P=0.027),이MICA화p53단백적표체정부상관(r=-0.23,P=0.047).결론 방광암중MICA단백상표체상조,가작위방광암적종류상관항원；NF-κB통로가능삼여MICA적표체조공；p53통로가능불삼여방광뇨로상피악성전화과정중MICA단백적표체.
Objective To investigate the mechanism of the expression of MHC class Ⅰ chain-related A (MICA) in carcinoma of the urinary bladder,the relationship between MICA,nuclear factor-κB ( NF-κB) and p53 expression in bladder cancer was studied.Methods The expression of MICA,NF-κB and p53 in a total of 75 cases of urothelial carcinoma tissues and 15 normal mucous membrane tissues of the bladder was evaluated by immunohistochemistry.Results The expression rates of MICA,NF-κB and p53 protein in urothelial carcinoma were 4.08％,85.3％ and 49.3％,respectively.Up-regulation of their expression was found in urothelial carcinoma compared with normal mucous membrane tissues ( P ＜ 0.05 ).MICA expression was positively correlated with NF-κB expression( r =0.256,P =0.027),but negatively correlated with p53 expression( r =- 0.23,P =0.047 ).Conclusions Up-regulation of MICA expression was detected in bladder cancer.MICA protein may be a new tumor-associated antigen of bladder cancer.MICA expression may be regulated by NF-κB pathway,while p53 pathway may not play a part in MICA up-expression during the urothelial malignant transformation.
