中国基层医药
中國基層醫藥
중국기층의약
CHINESE JOURNAL OF PRIMARY MEDICINE AND PHARMACY
2012年
10期
1460-1461
,共2页
肾肿瘤%血管增生%特异性环氧化酶2%血管内皮生长因子%成纤维细胞生长因子%微血管密度
腎腫瘤%血管增生%特異性環氧化酶2%血管內皮生長因子%成纖維細胞生長因子%微血管密度
신종류%혈관증생%특이성배양화매2%혈관내피생장인자%성섬유세포생장인자%미혈관밀도
Renal tumor%Cyclooxygenase-2-specific%Vascular endothelial growth factor%Fibroblast growth factor%Microvessel density
目的 探讨特异性环氧化酶2(COX-2)在肾细胞癌的表达及其与肾细胞癌血管增生的关系.方法 免疫组化检测肾细胞癌组织标本(85例)以及癌旁正常组织标本(48例)COX-2、血管内皮生长因子(VEGF)和碱性成纤维细胞生长因子(bFGF)的表达,CD34单克隆抗体标记血管内皮细胞进行微血管密度(MVD)计数.结果 COX-2、VEGF和bFGF在癌旁正常组织中阳性表达率分别为16.7％,20.8％和22.9％,在肾细胞癌组织阳性表达率分别为77.6％、80.0％和74.1％,两组差异均有统计学意义(均P＜0.01).肾癌组织MVD为(55.47±18.47),高于癌旁正常组织的(20.42±8.72)(P＜0.01).COX-2、VEGF、bFGF表达阳性的肾细胞癌其MVD均大于表达阴性的肾细胞癌MVD(P＜0.05或P＜0.01).肾细胞癌组织COX-2表达与VEGF、bFGF表达呈显著正相关(P＜0.01).结论 COX-2在肾细胞癌组织中表达升高,与肾细胞癌血管增生密切相关.
目的 探討特異性環氧化酶2(COX-2)在腎細胞癌的錶達及其與腎細胞癌血管增生的關繫.方法 免疫組化檢測腎細胞癌組織標本(85例)以及癌徬正常組織標本(48例)COX-2、血管內皮生長因子(VEGF)和堿性成纖維細胞生長因子(bFGF)的錶達,CD34單剋隆抗體標記血管內皮細胞進行微血管密度(MVD)計數.結果 COX-2、VEGF和bFGF在癌徬正常組織中暘性錶達率分彆為16.7％,20.8％和22.9％,在腎細胞癌組織暘性錶達率分彆為77.6％、80.0％和74.1％,兩組差異均有統計學意義(均P＜0.01).腎癌組織MVD為(55.47±18.47),高于癌徬正常組織的(20.42±8.72)(P＜0.01).COX-2、VEGF、bFGF錶達暘性的腎細胞癌其MVD均大于錶達陰性的腎細胞癌MVD(P＜0.05或P＜0.01).腎細胞癌組織COX-2錶達與VEGF、bFGF錶達呈顯著正相關(P＜0.01).結論 COX-2在腎細胞癌組織中錶達升高,與腎細胞癌血管增生密切相關.
목적 탐토특이성배양화매2(COX-2)재신세포암적표체급기여신세포암혈관증생적관계.방법 면역조화검측신세포암조직표본(85례)이급암방정상조직표본(48례)COX-2、혈관내피생장인자(VEGF)화감성성섬유세포생장인자(bFGF)적표체,CD34단극륭항체표기혈관내피세포진행미혈관밀도(MVD)계수.결과 COX-2、VEGF화bFGF재암방정상조직중양성표체솔분별위16.7％,20.8％화22.9％,재신세포암조직양성표체솔분별위77.6％、80.0％화74.1％,량조차이균유통계학의의(균P＜0.01).신암조직MVD위(55.47±18.47),고우암방정상조직적(20.42±8.72)(P＜0.01).COX-2、VEGF、bFGF표체양성적신세포암기MVD균대우표체음성적신세포암MVD(P＜0.05혹P＜0.01).신세포암조직COX-2표체여VEGF、bFGF표체정현저정상관(P＜0.01).결론 COX-2재신세포암조직중표체승고,여신세포암혈관증생밀절상관.
Objective To explore the expression of Cyclooxygenase-2-specific(COX-2) in renal cell carcinoma and its relationship with angiogenesis.Methods The expression of COX-2,VEGF and bFGF in renal cell carcinoma specimens(85 cases) and adjacent normal tissue samples(48 cases) was measured by immunohistochemistry.Microvessel density (MVD) was evaluated by immunohistochemistry using a monoclonal antibody against CD34.Results The positive expression rates of COX-2,VEGF and bFGF in adjacent normal tissue were 16.7％,20.8％,22.9％,and 77.6％,80.0％,74.1％ in renal cell carcinoma,respectively,the difference,were significant ( all P ＜0.01 ).MVD was (55.47 + 18.47 ) Counts/field in renal cell carcinoma,and ( 20.42 ± 8.72) Counts/field in adjacent normal tissue,the difference was significant( P ＜ 0.01 ).MVD in renal cell carcinoma which was positive expressed COX-2,VEGF,bFGF was higher than renal cell carcinoma which was negative expressed COX-2,VEGF,bFGF,the difference was significantly statistical( P ＜ 0.05 or P ＜0.01 ).COX-2 expression and VEGF,bFGF in renal cell carcinoma expression was positively correlated( P ＜0.01 ).Conclusion COX-2 expression was elevated in renal cell carcinoma,and it was related closely with renal cell carcinoma angiogenesis.
