中国地方病学杂志
中國地方病學雜誌
중국지방병학잡지
CHINESE JOURNAL OF ENDEMIOLOGY
2009年
3期
280-282
,共3页
李文桂%王鸿%朱佑明%杨梅
李文桂%王鴻%硃祐明%楊梅
리문계%왕홍%주우명%양매
棘球蚴病,肝%BCG-EmⅡ/3疫苗%BCG-Em14-3-3疫苗%受体,IgG%受体,IgE
棘毬蚴病,肝%BCG-EmⅡ/3疫苗%BCG-Em14-3-3疫苗%受體,IgG%受體,IgE
극구유병,간%BCG-EmⅡ/3역묘%BCG-Em14-3-3역묘%수체,IgG%수체,IgE
Echinococcosis,hepatic%BCG-EmⅡ/3 vaccine%BCG-Em14-3-3 vaccine%Receptors,IgG%Receptors,IgE
目的 动态观察多房棘球绦虫混合重组BCG-Em Ⅱ/3和BCG-Em14-3-3疫苗免疫小鼠后IgG及其业类和IgE的变化.方法 12～14周龄、体质量20～25 g的雌性Balb/c小鼠40只,鼻腔内接种上述疫苗,在0、2、4、6、8、10、12、14、16和18周按体质量随机剖杀4只小鼠,经眼球取血,常规酶联免疫吸附试验法测定血清中IgG及其亚类和IgE水平.结果 Balb/c小鼠的血清IgG、IgG2a和IgG2b水平均在免疫后第2～18周升高,并分别在免疫后第10、4和4周达到最高水平,其值分别为0.095±0.033、0.022±0.001和0.023±0.003,与0周(0.030±0.013、0.012±0.004和0.013±0.004)比较,差异有统计学意义(q值分别为2.95、4.87和2.81,P<0.05或<0.01);血清IgG1、IgG3和IgE水平均在免疫后2～18周降低,并分别在免疫后4、2和6周达较低水平,其值分别为0.031±0.004、0.136±0.002和0.114±0.002,与0周(0.192±0.007、0.175±0.013和0.024±0.003)比较,差异有统计学意义(q值分别为5.16、4.93和5.32,P<0.05或<0.01).结论 多房棘球绦虫混合重组BCG-EmⅡ/3和BCG-Em14-3-3疫苗在免疫早期即可诱导小鼠产生辅助性T细胞(TH)Ⅰ型保护性免疫反应.
目的 動態觀察多房棘毬縚蟲混閤重組BCG-Em Ⅱ/3和BCG-Em14-3-3疫苗免疫小鼠後IgG及其業類和IgE的變化.方法 12～14週齡、體質量20～25 g的雌性Balb/c小鼠40隻,鼻腔內接種上述疫苗,在0、2、4、6、8、10、12、14、16和18週按體質量隨機剖殺4隻小鼠,經眼毬取血,常規酶聯免疫吸附試驗法測定血清中IgG及其亞類和IgE水平.結果 Balb/c小鼠的血清IgG、IgG2a和IgG2b水平均在免疫後第2～18週升高,併分彆在免疫後第10、4和4週達到最高水平,其值分彆為0.095±0.033、0.022±0.001和0.023±0.003,與0週(0.030±0.013、0.012±0.004和0.013±0.004)比較,差異有統計學意義(q值分彆為2.95、4.87和2.81,P<0.05或<0.01);血清IgG1、IgG3和IgE水平均在免疫後2～18週降低,併分彆在免疫後4、2和6週達較低水平,其值分彆為0.031±0.004、0.136±0.002和0.114±0.002,與0週(0.192±0.007、0.175±0.013和0.024±0.003)比較,差異有統計學意義(q值分彆為5.16、4.93和5.32,P<0.05或<0.01).結論 多房棘毬縚蟲混閤重組BCG-EmⅡ/3和BCG-Em14-3-3疫苗在免疫早期即可誘導小鼠產生輔助性T細胞(TH)Ⅰ型保護性免疫反應.
목적 동태관찰다방극구조충혼합중조BCG-Em Ⅱ/3화BCG-Em14-3-3역묘면역소서후IgG급기업류화IgE적변화.방법 12～14주령、체질량20～25 g적자성Balb/c소서40지,비강내접충상술역묘,재0、2、4、6、8、10、12、14、16화18주안체질량수궤부살4지소서,경안구취혈,상규매련면역흡부시험법측정혈청중IgG급기아류화IgE수평.결과 Balb/c소서적혈청IgG、IgG2a화IgG2b수평균재면역후제2～18주승고,병분별재면역후제10、4화4주체도최고수평,기치분별위0.095±0.033、0.022±0.001화0.023±0.003,여0주(0.030±0.013、0.012±0.004화0.013±0.004)비교,차이유통계학의의(q치분별위2.95、4.87화2.81,P<0.05혹<0.01);혈청IgG1、IgG3화IgE수평균재면역후2～18주강저,병분별재면역후4、2화6주체교저수평,기치분별위0.031±0.004、0.136±0.002화0.114±0.002,여0주(0.192±0.007、0.175±0.013화0.024±0.003)비교,차이유통계학의의(q치분별위5.16、4.93화5.32,P<0.05혹<0.01).결론 다방극구조충혼합중조BCG-EmⅡ/3화BCG-Em14-3-3역묘재면역조기즉가유도소서산생보조성T세포(TH)Ⅰ형보호성면역반응.
Objective To dynamically observe changes of IgG, its subclasses and IgE in sera of mice by immunization with mixed recombinant of BCG-Em Ⅱ/3 and BCG-Em14-3-3 vaccine of Echinococcus multilocularis (Era). Methods Forty Balb/c mice of 12-14 week old and 20-25 g weight were intranasally vaccinated by the vaccine, 4 mice were killed randomly by the weight on 0,2,4,6,8,10,12,14,16 and 18 weeks of immunization respectively, sera were gathered from the eyeball to measure IgG, its subclasses and IgE by routine ELISA. Results Levels of IgG, IgG2a and IgG2b in the sera of mice increased obviously on 2-18 weeks, reached the highest level on 10, 4 and 4 weeks respectively, the value was 0.095±0.033,0.022±0.001,0.023±0.003 respectively, as compared with the value on 0 week(0.030±0.013,0.012±0.004,0.013±0.004), the difference being statistically significant(q=2.95,4.87,2.81 respectively, P < 0.01 or P < 0.05); levels of IgG1, IgG3 and IgE in the sera of mice decreased remarkably on 2-18 weeks,came to the lowest level on 4,2,6 weeks respectively, the value was 0.031±0.004,0.136±0.002,0.114±0.002 respectively, as compared with the value on 0 week(0.192±0.007, 0.175±0.013,0.024±0.003), the difference being statistically significant (q =5.16,4.93,5.32 respectively, P < 0.01 or P < 0.05). Conclusion Helper T cell(TH) Ⅰ response is induced in mice by mixed recombinant of BCG-Em Ⅱ/3 and BCG-Em14-3-3 vaccine on early immunization.
