国际医药卫生导报
國際醫藥衛生導報
국제의약위생도보
INTERNATIONAL MEDICINE & HEALTH GUIDANCE NEWS
2010年
16期
1943-1945
,共3页
异丙酚%机械通气%肿瘤坏死因子-α%白细胞介素-1
異丙酚%機械通氣%腫瘤壞死因子-α%白細胞介素-1
이병분%궤계통기%종류배사인자-α%백세포개소-1
Propofol%Ventilation%Tumor necrosis factor-α%Interleukin-1
目的 观察异丙酚对大鼠机械通气所致肺损伤及支气管肺泡灌洗液(BALF)肿瘤坏死因子α(TNF-α)、白介素-1 β(IL-1 β)水平的影响.方法 选择成年SD大鼠30只,随机分为对照组(C组)、机械通气组(V组)和机械通气+异丙酚组(P组),每组10只.C组以10 ml/kg潮气量进行机械通气,V组和P组设定潮气量为25 ml/kg以建立机械通气肺损伤模型.C组和V组在机械通气的同时输注5%葡萄糖溶液10 ml·kg-1·h-1,P组输注0.04%异丙酚10 ml·kg-1·h-1.机械通气4h后观察各组动物的肺湿/干重比值及BALF肿瘤坏死因子α(TNF-α)和白介素-1 β(IL-1 β)的浓度.结果 V组肺湿/干重比值以及BALF中TNF-α、IL-1 β的浓度均高于C组,P组肺湿/干重比值以及BALF中TNF-α、IL-1β的浓度均低于V组,与C组接近.结论 对大鼠进行25 ml/kg机械通气的同时输注0.04%异丙酚10 ml·kg-1·h-1,可以减轻机械通气所致肺损伤,其中的机制可能与抑制TNF-α、IL-1β等前炎症因子的释放有关.
目的 觀察異丙酚對大鼠機械通氣所緻肺損傷及支氣管肺泡灌洗液(BALF)腫瘤壞死因子α(TNF-α)、白介素-1 β(IL-1 β)水平的影響.方法 選擇成年SD大鼠30隻,隨機分為對照組(C組)、機械通氣組(V組)和機械通氣+異丙酚組(P組),每組10隻.C組以10 ml/kg潮氣量進行機械通氣,V組和P組設定潮氣量為25 ml/kg以建立機械通氣肺損傷模型.C組和V組在機械通氣的同時輸註5%葡萄糖溶液10 ml·kg-1·h-1,P組輸註0.04%異丙酚10 ml·kg-1·h-1.機械通氣4h後觀察各組動物的肺濕/榦重比值及BALF腫瘤壞死因子α(TNF-α)和白介素-1 β(IL-1 β)的濃度.結果 V組肺濕/榦重比值以及BALF中TNF-α、IL-1 β的濃度均高于C組,P組肺濕/榦重比值以及BALF中TNF-α、IL-1β的濃度均低于V組,與C組接近.結論 對大鼠進行25 ml/kg機械通氣的同時輸註0.04%異丙酚10 ml·kg-1·h-1,可以減輕機械通氣所緻肺損傷,其中的機製可能與抑製TNF-α、IL-1β等前炎癥因子的釋放有關.
목적 관찰이병분대대서궤계통기소치폐손상급지기관폐포관세액(BALF)종류배사인자α(TNF-α)、백개소-1 β(IL-1 β)수평적영향.방법 선택성년SD대서30지,수궤분위대조조(C조)、궤계통기조(V조)화궤계통기+이병분조(P조),매조10지.C조이10 ml/kg조기량진행궤계통기,V조화P조설정조기량위25 ml/kg이건립궤계통기폐손상모형.C조화V조재궤계통기적동시수주5%포도당용액10 ml·kg-1·h-1,P조수주0.04%이병분10 ml·kg-1·h-1.궤계통기4h후관찰각조동물적폐습/간중비치급BALF종류배사인자α(TNF-α)화백개소-1 β(IL-1 β)적농도.결과 V조폐습/간중비치이급BALF중TNF-α、IL-1 β적농도균고우C조,P조폐습/간중비치이급BALF중TNF-α、IL-1β적농도균저우V조,여C조접근.결론 대대서진행25 ml/kg궤계통기적동시수주0.04%이병분10 ml·kg-1·h-1,가이감경궤계통기소치폐손상,기중적궤제가능여억제TNF-α、IL-1β등전염증인자적석방유관.
Objective To explore the effect of propofol on the expressions of cytokines in ventila- tion-induced lung injury. Methods Thirty Sprague Dawley rats were randomly divided into three groups with 10 in each. Group C received 10 ml ·kg-1·h-1 of 5% glucose infusion while was ventilated with a tidal volume of 10mlAg· Group V was infused with glucose at the same dose with a tidal volume of 25 ml/kg. Group P received 10 ml· kg-1·h-1 of 0.04% propofol infusion and was ventilated with a tidal volume of 25 ml/kg. All the rats were sacrified 4 hours after infusion. The wet/dry lung weight ratio and levels of TNF- α and IL-1β in the bronchoalveolar lavage fluid were measured. Results The wet/dry lung weight ratio and levels of TNF- a and IL- 1 β ere significantly increased in group V as compared with those in group C (P< 0. 05). Propofol significantly reduced the wet/dry lung weight ratio and levels of TNF-α and IL-1 β in group P as compared with group V. Conclusions Propofol inhibits the increase of the wet/dry lung weight ratio and levels of TNF- α. and IL-1 β in the bronchoalveolar lavage fluid after high tidal volume ventilation.
