CAJ | 학술논문

目的观察支气管哮喘不同状态时外周淋巴细胞肾上腺素能受体（βAR）及β2ARmRNA水平的变化。方法用放射配基结合受体分析法，放射免疫法，逆转录聚合酶链反应（PT-PCR）及原位杂交等方法测定哮喘患者不同状态下β2AR密度，β2ARmRNA水平，血浆cAMP和cGMP水平。结果缓解期哮喘患者外周淋巴细胞βAR的密度较正常人明显升高，血浆cAMP水平无显著差异，而发作期哮喘患者βAR及血浆cAMP水平均较正常人明显降低；缓解期哮喘患者外周淋巴细胞β2ARmRNA水平与正常人无显著差异。结论βAR数量、功能及其信使核糖核酸水平与哮喘不同发病状态有关，其改变仅为哮喘病程中伴随出现的变化，不是其发病的原发因素。
목적관찰지기관효천불동상태시외주림파세포신상선소능수체（βAR）급β2ARmRNA수평적변화。방법용방사배기결합수체분석법，방사면역법，역전록취합매련반응（PT-PCR）급원위잡교등방법측정효천환자불동상태하β2AR밀도，β2ARmRNA수평，혈장cAMP화cGMP수평。결과완해기효천환자외주림파세포βAR적밀도교정상인명현승고，혈장cAMP수평무현저차이，이발작기효천환자βAR급혈장cAMP수평균교정상인명현강저；완해기효천환자외주림파세포β2ARmRNA수평여정상인무현저차이。결론βAR수량、공능급기신사핵당핵산수평여효천불동발병상태유관，기개변부위효천병정중반수출현적변화，불시기발병적원발인소。
Objective To investigate the changes of β-adrenergic receptor (βAR) in peripheral lymphocytes and β2AR mRNA levels at different stages of bronchial asthma. Methods β2AR density and β2AR mRNA level in peripheral lymphocytes, cAMP and cGMP levels in blood plasma were estimated by radioligand binding assay, radioimmunoassay and RT-PCR.Results (1) Maximum bound volume (Bmax) and equilibrium dissociation constant (Kd) of β2AR of lymphocyte in asthma patients at remission stage were markedly higher than that in normal subjects, while cAMP levels in blood plasma showed no difference. Bmax of β2AR and cAMP levels in asthma patients at acute exacerbation stage were significantly lower than that in normal subjects, and Kds between these two groups were not much different. (2) Expression of β2ARmRNA in peripheral lymphocytes of asthmatics at remission stage was not significantly different compared with that in normals. Conclusions Amount and function of βAR and β2ARmRNA levels are related to asthmatic conditions. Changes of βAR and β2ARmRNA in asthma might rather be a pathological change accompanied by the course of the disease than a primary defect.