CAJ | 학술논문

采用胶体金试纸条与液相色谱-串联质谱（LC-MS/MS）两种方法检测牛奶中残留的四环素类药物。结果表明：胶体金试纸条的检测限为四环素40μg/L、强力霉素40μg/L、金霉素40μg/L、土霉素80μg/L;LC-MS/MS法在牛奶中四环素、强力霉素、金霉素、土霉素的检测限为50μg/L。采用两种方法对实际样品检测,检测结果一致。与液相色谱-串联质谱法相比,胶体金试纸条具有操作简便、快速、直观的特点,可作为筛选法对样品进行定性筛查,LC-MS/MS法进行阳性样品的确证和精确定量。
채용효체금시지조여액상색보-천련질보（LC-MS/MS）량충방법검측우내중잔류적사배소류약물。결과표명：효체금시지조적검측한위사배소40μg/L、강력매소40μg/L、금매소40μg/L、토매소80μg/L;LC-MS/MS법재우내중사배소、강력매소、금매소、토매소적검측한위50μg/L。채용량충방법대실제양품검측,검측결과일치。여액상색보-천련질보법상비,효체금시지조구유조작간편、쾌속、직관적특점,가작위사선법대양품진행정성사사,LC-MS/MS법진행양성양품적학증화정학정량。
The two different analytical methods,colloidal gold strip（CGS） and liquid chromatography-tandem mass spectrometry（LC-MS/MS）,were used to determine tetracyclines residue in milk and the analytical results were compared.CGS showed a detection limit of 40 μg/L for tetracycline,chlortetracycline and doxycycline and of 80 μg/L for oxytetracycline.The detection limits of LC-MS/MS for the tetracyclines were all 50 μg/L.Identical results were obtained for the determination of real samples by both methods.Over LC-MS/MS,CGS had the advantages of ease of use,rapidity and visualization and could allow qualitative analysis of positive samples.LC-MS/MS was suitable for the validation of positive samples and accurate quantification.