陕西师范大学学报:自然科学版
陝西師範大學學報:自然科學版
협서사범대학학보:자연과학판
Journal of Shaanxi Normal University: Nat Sci Ed
2011年
5期
62-66
,共5页
胞外ATP%泡桐%花粉萌发%花粉管伸长%一氧化氮
胞外ATP%泡桐%花粉萌髮%花粉管伸長%一氧化氮
포외ATP%포동%화분맹발%화분관신장%일양화담
extracellular ATP(eATP)%Paulownia tomentosa%pollen germination%pollen tube growth%nitric oxide
研究了胞外ATP(eATP)对泡桐(Paulownia tomaentosa Steud.)花粉萌发和花粉管伸长的效应及其与一氧化氮(NO)的关系.结果表明,eATP和NO供体硝普钠(SNP)均显著抑制花粉萌发和花粉管伸长,且表现明显浓度和时间依赖效应.一氧化氮合酶(NOS)抑制剂(L-NAME)和NO清除剂(c-PTIO)能够部分阻止eATP对花粉萌发和花粉管伸长的抑制作用,显示eATP抑制花粉萌发和花粉管伸长与其诱导NO产生有关,eATP诱导的NO可能由NOS催化产生.
研究瞭胞外ATP(eATP)對泡桐(Paulownia tomaentosa Steud.)花粉萌髮和花粉管伸長的效應及其與一氧化氮(NO)的關繫.結果錶明,eATP和NO供體硝普鈉(SNP)均顯著抑製花粉萌髮和花粉管伸長,且錶現明顯濃度和時間依賴效應.一氧化氮閤酶(NOS)抑製劑(L-NAME)和NO清除劑(c-PTIO)能夠部分阻止eATP對花粉萌髮和花粉管伸長的抑製作用,顯示eATP抑製花粉萌髮和花粉管伸長與其誘導NO產生有關,eATP誘導的NO可能由NOS催化產生.
연구료포외ATP(eATP)대포동(Paulownia tomaentosa Steud.)화분맹발화화분관신장적효응급기여일양화담(NO)적관계.결과표명,eATP화NO공체초보납(SNP)균현저억제화분맹발화화분관신장,차표현명현농도화시간의뢰효응.일양화담합매(NOS)억제제(L-NAME)화NO청제제(c-PTIO)능구부분조지eATP대화분맹발화화분관신장적억제작용,현시eATP억제화분맹발화화분관신장여기유도NO산생유관,eATP유도적NO가능유NOS최화산생.
The role of eATP and its relationship with nitric oxide(NO) in pollen germination and tube growth of Paulownia tomentosa were studied.The results showed that both eATP and NO donors(sodium nitroprusside) inhibited pollen germination and tube growth in a dose-dependence manner.Both NOS inhibitor(L-NAME) and NO scavenger(c-PTIO) partly reversed eATP-inhibited pollen germination and tube growth,indicating that the inhibitory effects of eATP on pollen germination and tube growth are related to NO generated by a NOS-like enzyme in pollen.