CAJ | 학술논문

随着规模化养猪业的发展,传染性疾病越来越多,混合感染或多重感染十分普遍,给疾病的诊断和预防带来很大困难。本文将我们实验室2010年分别采自福建、广西、河南、上海、内蒙、浙江、江苏和山西等地发病猪场的185份病料,通过RT-PCR和PCR方法对其进行猪繁殖与呼吸综合征病毒（Porcine reproductive and respiratory syndrome virus,PRRSV）、猪繁殖障碍的猪瘟病毒（Classical swine fever virus,CSFV）、猪圆环病毒2（Porcine circovirus type 2,PCV2）、猪细小病毒（Porcine parvovirus,PPV）、猪伪狂犬病毒（Pseudorabies virus,PRV）、细环病毒2（Torque teno virus 2,TTV2）等病毒检测。结果表明在所检的病料中PRRSV、PCV2和TTV2的阳性率比较高,分别为49.2%、62.2%和95.1%。有些地方TTV2的阳性率高达100%;同时,还存在很普遍的PRRSV与PCV2、PRRSV与TTV2、PCV2与TTV2等混合感染,混合感染率分别为27.6%、45.4%、58.4%;以及PRRSV、PCV2与TTV2的三重感染率为24.9%。同时对部分PRRSV阳性病料的PRRSV GP5和nsp2基因分别进行测序,分析测序结果表明病料中的PRRSV的GP5和nsp2序列与PRRSV HuN4株的相应序列的亲缘关系分别在98.2%和96.2%以上,且在nsp2区域都存在30个不连续氨基酸的缺失,说明现在临床中所流行的PRRSV可能仍是与高致病性PRRSV基因型相似的病毒。通过本文可以及时了解当前养猪场的病毒性疾病的流行情况,为猪场病毒性疾病的防控提供依据。
수착규모화양저업적발전,전염성질병월래월다,혼합감염혹다중감염십분보편,급질병적진단화예방대래흔대곤난。본문장아문실험실2010년분별채자복건、엄서、하남、상해、내몽、절강、강소화산서등지발병저장적185빈병료,통과RT-PCR화PCR방법대기진행저번식여호흡종합정병독（Porcine reproductive and respiratory syndrome virus,PRRSV）、저번식장애적저온병독（Classical swine fever virus,CSFV）、저원배병독2（Porcine circovirus type 2,PCV2）、저세소병독（Porcine parvovirus,PPV）、저위광견병독（Pseudorabies virus,PRV）、세배병독2（Torque teno virus 2,TTV2）등병독검측。결과표명재소검적병료중PRRSV、PCV2화TTV2적양성솔비교고,분별위49.2%、62.2%화95.1%。유사지방TTV2적양성솔고체100%;동시,환존재흔보편적PRRSV여PCV2、PRRSV여TTV2、PCV2여TTV2등혼합감염,혼합감염솔분별위27.6%、45.4%、58.4%;이급PRRSV、PCV2여TTV2적삼중감염솔위24.9%。동시대부분PRRSV양성병료적PRRSV GP5화nsp2기인분별진행측서,분석측서결과표명병료중적PRRSV적GP5화nsp2서렬여PRRSV HuN4주적상응서렬적친연관계분별재98.2%화96.2%이상,차재nsp2구역도존재30개불련속안기산적결실,설명현재림상중소류행적PRRSV가능잉시여고치병성PRRSV기인형상사적병독。통과본문가이급시료해당전양저장적병독성질병적류행정황,위저장병독성질병적방공제공의거。
Infectious diseases have become the major problem with the development of large-scale pig industry in China.Multiple infections often occur on many pig farms.In the present study,185 samples collected from pigs of eight provinces in 2010 were tested in PCR or RT-PCR for porcine reproductive and respiratory syndrome virus（PRRSV）,classical swine fever virus（CSFV）,porcine circovirus type 2（PCV2）,porcine parvovirus（PPV）,pseudorabies virus（PRV） and Torque teno virus 2（TTV2）.The results showed that positive rates for PCV2,PRRSV and TTV2 were 49.2%,62.2% and 95.1%,respectively.In some swine herds,positive rate of TTV2 infection was 100%.In addition,mixed infections were found common with 27.6% for PRRSV/PCV2,45.4% for PRRSV/TTV2,58.4% for PCV2/TTV2 and even 24.9% for PCV2/TTV2/PRRSV.Furthermore,sequence analysis of GP5 and nsp2 genes from some of PRRSV positive samples revealed 98.2% or 96.2% identities with those of the highly pathogenic PRRSV（HP-PRRSV） HuN4 strain and a discontinued deletion of 30 amino acids in nsp2 region,indicating widespread of HP-PRRSV in pig herds.The information obtained from these investigations can be used to outline prevention and control of viral diseases.