CAJ | 학술논문

本文开展了FITC染料编码流式聚苯乙烯微球的研究。研究结果显示,制备的聚苯乙烯微球粒径为5.4μm,变异系数为3.04%。当低亮和高亮流式聚苯乙烯微球分别用0.4μL和125μL的FITC母液染色时间0.5 h时,可以得到微球群集中、荧光强度变异系数分别为5.28%4、.63%的二元流式微球阵列。FITC染色编码微球保存12个月,保留荧光强度为90%以上。
본문개전료FITC염료편마류식취분을희미구적연구。연구결과현시,제비적취분을희미구립경위5.4μm,변이계수위3.04%。당저량화고량류식취분을희미구분별용0.4μL화125μL적FITC모액염색시간0.5 h시,가이득도미구군집중、형광강도변이계수분별위5.28%4、.63%적이원류식미구진렬。FITC염색편마미구보존12개월,보류형광강도위90%이상。
The action of FITC to flow functional beads encoding was studied in this paper.Results from this study showed that produced microbeads had the average diameter of 5.4 μm and 3.04% CV value.Flow cytometry exhibited that the high-light and low-light bead,which constructed the duplexed bead array with 5.28%,4.63% CV value,can be successfully encoded by 0.4 μL and 125 μL FITC fluorescence solution for half an hour.Ninety percent of FITC density can be kept after 12 months.