CAJ | 학술논문

使用酶法合成了一系列不同烷基链长的β-D-烷基葡萄糖苷.结果表明,除甲基糖苷外,烷基碳链越长,反应初速度越低,糖苷的最终收率越低.从热力学角度系统地研究了这些糖苷合成的平衡常数和吉布斯自由能变化.通过改变反应的平衡,提高了长链烷基糖苷的最终收率.当水含量从10％降至5％ (v/v),使用叔丁醇作为共溶剂,添加0.1 mol/L葡萄糖作为底物时,癸基糖苷的平衡得率从1.9％提高至6.1％.除了其他长链的烷基糖苷外,庚基糖苷也显示了明显的表面活性剂活性.
사용매법합성료일계렬불동완기련장적β-D-완기포도당감.결과표명,제갑기당감외,완기탄련월장,반응초속도월저,당감적최종수솔월저.종열역학각도계통지연구료저사당감합성적평형상수화길포사자유능변화.통과개변반응적평형,제고료장련완기당감적최종수솔.당수함량종10％강지5％ (v/v),사용숙정순작위공용제,첨가0.1 mol/L포도당작위저물시,계기당감적평형득솔종1.9％제고지6.1％.제료기타장련적완기당감외,경기당감야현시료명현적표면활성제활성.
An array of ten β-D-glucopyranosides with varied alkyl chain lengths were enzymatically synthesized.It was found that for longeralkyl chains a lower initial rate and final yield of glucoside was obtained except for methyl glucoside because of the severe toxicity of methanol to the enzyme.From a thermodynamics point of view,the equilibrium constant and Gibbs free energy variation of the glucoside syntheses were systematically investigated.To improve the final yields of the glucosides containing long alkyl chains the equilibrium of the enzymatic glucoside synthesis was altered.The equilibrium yield of decyl β-D-glucoside increased from 1.9％ to 6.1％ when the water content was reduced from 10％ to 5％ (v/v) using tert-butanol as a cosolvent and 0.10 mol/L of glucose as a substrate.As for the other longer alkyl chain glucosides,heptyl β-D-glucoside was found to have significant surface activity as well.