CAJ | 학술논문

目的探讨胰岛素样生长因子1（IGF-1）对人非小细胞肺癌（NSCLC）细胞增殖的影响及可能的分子机制。方法 MTT比色法检测0.1,1,10,100 ng/ml IGF-1对非小细胞肺癌系腺癌A549、鳞癌LK2、大细胞肺癌H460细胞增殖的影响,流式细胞术（FCM）、Western blot分析IGF-1处理前后各肺癌细胞的细胞周期变化,S期激酶相关蛋白2（SKP2）和CDC20同源蛋白1（CDH1）的表达变化。结果在非小细胞肺癌系A549、LK2、H460中,不同浓度的IGF-1均显示有促进细胞增殖的作用（P〈0.05）,其中1 ng/ml IGF-1作用后细胞增殖率达到峰值;与DMSO组相比,处理组S期细胞增加（P〈0.01）,SKP2蛋白表达增加（P〈0.05）,但CDH1蛋白表达却无明显变化（P〉0.05）。结论非小细胞肺癌细胞中IGF-1可能是通过SKP2蛋白表达的增强,负性调控p27作用,加速细胞周期的进程。
목적탐토이도소양생장인자1（IGF-1）대인비소세포폐암（NSCLC）세포증식적영향급가능적분자궤제。방법 MTT비색법검측0.1,1,10,100 ng/ml IGF-1대비소세포폐암계선암A549、린암LK2、대세포폐암H460세포증식적영향,류식세포술（FCM）、Western blot분석IGF-1처리전후각폐암세포적세포주기변화,S기격매상관단백2（SKP2）화CDC20동원단백1（CDH1）적표체변화。결과재비소세포폐암계A549、LK2、H460중,불동농도적IGF-1균현시유촉진세포증식적작용（P〈0.05）,기중1 ng/ml IGF-1작용후세포증식솔체도봉치;여DMSO조상비,처리조S기세포증가（P〈0.01）,SKP2단백표체증가（P〈0.05）,단CDH1단백표체각무명현변화（P〉0.05）。결론비소세포폐암세포중IGF-1가능시통과SKP2단백표체적증강,부성조공p27작용,가속세포주기적진정。
Objective To investigate the effects of insulin-like growth factor-1（IGF-1）on the cell proliferation of human non-small-cell lung cancer（NSCLC） and the possible molecular mechanism.Methods MTT assay was used to examine the effects of IGF-1 （0.1,1,10,100 ng/mL）on the cell proliferation of NSCLC cell lines（A549,LK2,H460）,Flow cytometry（FCM）and Western blot to ana-lyze the cell cycles and the protein expression of S-Phase Kinase-Associated Proteins 2（Skp2）and CDC20 homolog 1（CDH1）,respectively.Results The cell proliferation of NSCLC cell lines（A549,LK2,H460）could be promoted by the IGF-1 at different concentrations and the proliferation rate peaked when the cells were treated with 1 ng/mL IGF-1.Compared with control,the percentage of the S-phase cell population was significantly increased after the treatment of IGF-I（P 〈 0.01）and the protein expression of SKP2 also increased obviously（P 〈0.05）.However,there was no change in the CDH1 protein expression（P 〉 0.05）.Conclusion IGF-1 may accelerate the cell-cycle pro-gression of NSCLC cells by negatively modulating p27 protein via the up-regulation of SKP2 protein expression.